Methods: ELISA, IB
(Claus U. Pietrzik, Institute of Physiological Chemistry and Pathobiochemistry, Molecular Neurodegeneration, Johannes-Gutenberg-University Mainz, Mainz, Germany)
Richter L, 2010
Reacts in: Human
immunogen = synthetic human Ab peptide 1–16, cross-linked to keyhole limpet hemocyanin
recognizes residues 1-16 of Amyloid β. Hybridoma cells were generated by standard procedures and screened for recognition of HSA cross-linked to Ab 1-16 by ELISA; recognition of synthetic Ab 1–42 on a standardized dot blot procedure was conﬁrmed and the epitope mapped to Ab 1–8 using a peptide array spanning the entire sequence of Ab
- Richter L, Munter LM, Ness J, Hildebrand PW, Dasari M, Unterreitmeier S, Bulic B, Beyermann M, Gust R, Reif B, Weggen S, Langosch D, Multhaup G. Amyloid beta 42 peptide (Abeta42)-lowering compounds directly bind to Abeta and interfere with amyloid precursor protein (APP) transmembrane dimerization. Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14597-602. PubMed.
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