Helmholtz Antibody Collection
The following are selected lists of antibodies against microglial proteins that are available through the Monoclonal Antibody Core Facility at Helmholtz Munich.
ABI3 | ApoE | CD33 | CLEC7A | CX3CR1 | P2Y12R | TMEM119 | TYROBP/DAP12
Tested Applications:
WB(O) - Western Blot (Overexpressed) | WB (E) - Western Blot (Endogenous) | IP (O) - Immunoprecipitation (Overexpressed) | IP (E) Immunoprecipitation (Endogenous) | IF - Immunofluorescene (Overexpressed) | IF - Immunofluorescene (Endogenous) | IH - Immunohistochemistry | KO - Knocked Out Validated
The “+” signs in the tables below represent subjective ratings of the strengths of the signals generated by the antibodies in the tested applications, provided by the scientists who tested the antibodies. The ratings are intended only to provide loose guidance to others interested in using these antibodies, as results are expected to depend upon experimental conditions and may vary between laboratories.
ABI3
Clonality: monoclonal | Immunized Antigen Localization:Peptide in C-terminus| (Project ID: ABIM)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3C4 | mouse | rat | IgG2a | ++ | |||||||
| 6F7 | mouse | rat | IgG2a | ++ | ++ | + | + (spleen WB) |
Clonality: monoclonal | Immunized Antigen Localization: rec. Protein | (Project ID: ABISP)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 30B7 | human/mouse | mouse | IgG1 | +++ | ++ | ++ | + | + (spleen WB) | |||
| 12B2 | human | rat | IgG2b | +++ | + | ||||||
| 22B9 | human | rat | IgG2b | +++ | + |
ApoE
Clonality: monoclonal | Immunized Antigen Localization: Peptide in N-terminus | (Project ID: APO1M)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 26C11 | mouse | mouse | IgG2b | +++ | + |
Clonality: monoclonal | Immunized Antigen Localization: Peptide in N-terminus | (Project ID: APO1S)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 6B9 | human | rat | IgG2a | +++ | + | ||||||
| 6C10 | human | rat | IgG2a | +++ |
Clonality: monoclonal | Immunized Antigen Localization: rec. Protein | (Project ID: APOE)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8D4 | human | rat | IgG2a | ++ | |||||||
| 5E8 | human | rat | IgG2a | +++ | + | ||||||
| 28A11 | human | mouse | IgG1 | +++ |
CD33
Clonality: monoclonal | Immunized Antigen Localization: Isoform 2, peptide in C-terminus| (Project ID: CD33B)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 8C1 | mouse | rat | IgG2c | +++ | ++ | + | |||||
| 11A8 | mouse | rat | IgG2c | +++ | ++ | ||||||
| 20H2 | mouse | rat | IgG2c | +++ | ++ | ||||||
| 3F5 | mouse | rat | IgG2c | +++ | ++ |
Clonality: monoclonal | Immunized Antigen Localization: Epitope in all three annotated transcripts, peptide in C-terminus | (Project ID: CD33 S)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2C11 | human | rat | IgG2b | +++ | +++ | ||||||
| 10B2 | human | rat | IgG2c | ++* | +++* | ||||||
| 24F3 | human | rat | IgG2b | ++* | +++* | + | |||||
| 12C1 | human | rat | IgG2c | ++ | +++*** | + | |||||
| 17D5 | human | rat | IgG2c | +++ | +++*** | + |
*preferentially detects CD33m
**detects all three CD33 isoforms, but CD33m > CD33M, CD33-3
*** detects all three CD33 isoforms, but CD33M, CD33m > CD33-3
CLEC7A
Clonality: monoclonal | Immunized Antigen Localization: Cytoplasmatic peptide | (Project ID: CLCMA)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1F1 | mouse | rat | IgG2a | +++ |
++ (BV2), ++ (primary microglia), +++ (mouse brain) |
+ (mouse brain) |
Clonality: monoclonal | Immunized Antigen Localization: Cytoplasmatic peptide | (Project ID: CLCSA)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5B10 | human | rat | IgG2b | ++ |
CX3CR1
Clonality: monoclonal | Immunized Antigen Localization: Peptide in C-terminus | (Project ID: CX3S)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 27C3 | human | mouse | IgG3 | + (only tagged) | |||||||
| 22C7 | human | rat | IgG2c | + (only tagged) |
P2Y12R
Clonality: monoclonal | Immunized Antigen Localization: Peptide in C-terminus | (Project ID: P2YM)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 17A12 | mouse | rat | IgG2c | + | ++ (mouse brain slices) | ||||||
| 1E5 | mouse | rat | IgG2c | + | ++ (mouse brain slices) |
TMEM119
Clonality: monoclonal | Immunized Antigen Localization: Cytoplasmatic peptide | (Project ID: TMECS)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1G4 | human | rat | IgG2b | ++ | |||||||
| 23B6 | human | rat | IgG2a | + | |||||||
| 5A4 | human | rat | IgG2c | + |
TYROBP/DAP12
Clonality: monoclonal | Immunized Antigen Localization: Peptide in C-terminus | (Project ID: DAPCS/M)
| Clone ID | Specificity | Host | Isotype | WB (O) | WB (E) | IP (O) | IP (E) | IF (O) | IF (E) | IH | KO |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1C8 | human/mouse | rat | IgG2c | +++ (BV2) | + | + | BV2 KO cells, lysate of membrane preps | ||||
| 4E12 | human/mouse | rat | IgG2c | +++ (BV2) | + | + | BV2 KO cells, lysate of membrane preps | ||||
| 14C2 | human/mouse | rat | IgG2c | +++ (BV2) | + | + | + | BV2 KO cells, lysate of membrane preps | |||
| 9D10 | human/mouse | rat | IgG2c | +++ (BV2) | + | + | BV2 KO cells, lysate of membrane preps |