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Thioflavin S Staining

PROTOCOL This protocol describes how to use Thioflavin S to detect plaques and tangles in brain tissue. dselkoe Harvard Medical School and Brigham & Women's Hospital plaques, tangles, beta-pleated sheet, histology protocol_Thioflavin_S_staining_of_brain_t

Strip & Reprobe of PVDF Membranes

PROTOCOL This protocol describes how to strip the antibodies from a PVDF membrane so that another protein can be assessed on the same blot. dselkoe Harvard Medical School and Brigham and Women's Hospital PVDF, polyvinylidene difluoride, western, immunoblot pr

Neprilysin Activity Assay

PROTOCOL This is a protocol for measuring the enzymatic activity of neprilysin, an Aβ-degrading enzyme. dselkoe Harvard Medical School and Brigham & Women's Hospital Reducing amyloid plaque burden via ex vivo gene delivery of an Abeta-degrading protease:

MDCK Cell Monolayer and Aβ Transport Assay

PROTOCOL This is a protocol for culturing MDCK cells (an epithelial cell line derived from the canine kidney) in a monolayer and assessing Trans-Epithelial Electrical Resistance (TEER) and Aβ transport. dselkoe Harvard Medical School and Brigham & Women's

Immunoprecipitation

PROTOCOL A protocol for enriching for a protein of interest by taking advantage of the specific interaction of an antigen with an antibody. dselkoe Harvard Medical School and Brigham & Women's Hospital IP, Immunoprecipitation protocol_Immunoprecipitation.

Immunohistochemistry (Immunoperoxidase)

PROTOCOL A method for detecting proteins of interest in brain sections using an immunoperoxidase reaction. dselkoe Harvard Medical School and Brigham & Women's Hospital IHC, Immunohistochemistry, brain protocol_Immunohistochemistry.pdf Immunostaining

Aβ ELISA

PROTOCOL A protocol to detect Aβ in tissue culture supernatant and in homogenized tissue. dselkoe Harvard Medical School and Brigham & Women's Hospital Aβ, ELISA, protocol_Abeta_ELISA.pdf ELISA

Coating of Transwell Filters

PROTOCOL A protocol to coat filters with fibronectin and collagen for cell culture. dselkoe Harvard Medical School and Brigham & Women's Hospital LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-beta peptide in a blood-brain

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