PROTOCOL This protocol can be used to generate an affinity matrix consisting of an antibody covalently linked to Protein A/G-coated beads. dselkoe Harvard Medical School and Brigham & Women's Hospital protein A/G, immunoprecipitation protocol_Coupling_Ant
PROTOCOL A protocol for culturing rodent fibroblasts. dselkoe Harvard Medical School and Brigham & Women's Hospital fibroblasts, rodent, cell culture protocol_Culturing_Embryonic_Fibroblasts.pdf Cell Culture
PROTOCOL A method for isolating DNA from cells. dselkoe Harvard Medical School and Brigham & Women's Hospital DNA protocol_DNA_ethanol_precipitation.pdf Nucleic Acid Isolation/Purification
PROTOCOL Extraction of human Aβ from APP transgenic mouse brain into TBS-soluble, guanidine HCI-soluble fractions. dselkoe Harvard Medical School Aβ, APP, transgenic mice protocol_Extraction_of_human_Abeta_from_APP_Tg_mouse_brain.pdf Protein Isolation/Extraction A
PROTOCOL A method for detecting Aβ in human samples dselkoe Harvard Medical School and Brigham and Women's Hospital Aβ, ELISA, human protocol_96_Well_Abeta_ELISA.pdf ELISA APP
CONFERENCE COVERAGE 2013-07-19 Conference Coverage Despite rampant plaque pathology, most transgenic mice that overproduce amyloidβ (Aβ) fail to capture other hallmarks of Alzheimer’s disease, such as neurofibrillary tangles and neuronal loss. Now, a study in the July 17 Science Transl