Western Blotting
PROTOCOL This protocol uses the Bio-Rad blotting system. dselkoe Harvard Medical School and the Brigham and Women's Hospital Western blot, PVDF, Transfer buffer protocol_Western_blotting.pdf Western Blotting
330026 RESULTS
PROTOCOL This protocol uses the Bio-Rad blotting system. dselkoe Harvard Medical School and the Brigham and Women's Hospital Western blot, PVDF, Transfer buffer protocol_Western_blotting.pdf Western Blotting
PROTOCOL Frequently used on human and mouse brain sections for detecting pathologies in the endocytic-autophagic-lysosomal pathways such as changes in the numbers, types and distribution of vacuoles, and expression levels of individual molecules in the pathways. n
PROTOCOL Uptake of HRP into neuronal cells occurs via bulk endocytosis, especially at axonal terminals. Endocytosed HRP is transported in a retrograde direction and delivered into autophagic-lysosomal vacuoles in the cell body and, therefore, can serve as a marker
PROTOCOL In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in living cells. nixon New York University School of Medicine and the Nathan Kline Institute Probing the cathepsin D using a BODIPY FL-pepstatin A: applications i
PROTOCOL Autophagic-lysosomal compartments contain numerous acid hydrolases. Analysis using enzyme histochemistry for acid phosphatase at the ultrastructural level can provide information about the trafficking and distribution of acid hydrolases in neuronal proces