PAPER Pandya RS, Zhu H, Li W, Bowser R, Friedlander RM, Wang X
SEARCH RESULTS
330026 RESULTS
Thioflavin S Staining
PROTOCOL This protocol describes how to use Thioflavin S to detect plaques and tangles in brain tissue. dselkoe Harvard Medical School and Brigham & Women's Hospital plaques, tangles, beta-pleated sheet, histology protocol_Thioflavin_S_staining_of_brain_t
Strip & Reprobe of PVDF Membranes
PROTOCOL This protocol describes how to strip the antibodies from a PVDF membrane so that another protein can be assessed on the same blot. dselkoe Harvard Medical School and Brigham and Women's Hospital PVDF, polyvinylidene difluoride, western, immunoblot pr
Preparation of Mouse Brain for Biochemical and Histological Analysis
PROTOCOL A short protocol for preparing mouse brain for analysis by preserving one hemisphere in liquid nitrogen and the other in 10% formalin. dselkoe Harvard Medical School and Brigham and Women's Hospital Deciphering Alzheimer disease. formalin, liquid nit
Neprilysin Activity Assay
PROTOCOL This is a protocol for measuring the enzymatic activity of neprilysin, an Aβ-degrading enzyme. dselkoe Harvard Medical School and Brigham & Women's Hospital Reducing amyloid plaque burden via ex vivo gene delivery of an Abeta-degrading protease:
MDCK Cell Monolayer and Aβ Transport Assay
PROTOCOL This is a protocol for culturing MDCK cells (an epithelial cell line derived from the canine kidney) in a monolayer and assessing Trans-Epithelial Electrical Resistance (TEER) and Aβ transport. dselkoe Harvard Medical School and Brigham & Women's
Diana Himmelstein
Northwesten UniversityUnited States
PAPER Propper DJ, Braybrooke JP, Taylor DJ, Lodi R, Styles P, Cramer JA, Collins WC, Levitt NC, Talbot DC, Ganesan TS, Harris AL
Phase I trial of the selective mitochondrial toxin MKT077 in chemo-resistant solid tumours.
Ann Oncol. 1999 Aug;10(8):923-7. PubMed: 10509153Immunoprecipitation
PROTOCOL A protocol for enriching for a protein of interest by taking advantage of the specific interaction of an antigen with an antibody. dselkoe Harvard Medical School and Brigham & Women's Hospital IP, Immunoprecipitation protocol_Immunoprecipitation.
Immunohistochemistry (Immunoperoxidase)
PROTOCOL A method for detecting proteins of interest in brain sections using an immunoperoxidase reaction. dselkoe Harvard Medical School and Brigham & Women's Hospital IHC, Immunohistochemistry, brain protocol_Immunohistochemistry.pdf Immunostaining
Aβ ELISA
PROTOCOL A protocol to detect Aβ in tissue culture supernatant and in homogenized tissue. dselkoe Harvard Medical School and Brigham & Women's Hospital Aβ, ELISA, protocol_Abeta_ELISA.pdf ELISA
Coating of Transwell Filters
PROTOCOL A protocol to coat filters with fibronectin and collagen for cell culture. dselkoe Harvard Medical School and Brigham & Women's Hospital LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-beta peptide in a blood-brain
Neil Andrews
Pain Research ForumBoston, United States
ACE Activity Assay
PROTOCOL A method to measure angiotensin-converting enzyme (ACE) activity. dselkoe Harvard Medical School and Brigham & Women's Hospital An improved fluorometric assay of rat serum and plasma converting enzyme. ACE, angiotensin-converting enzyme, Aβ degra
Collagenase/Dispase Mouse Brain Isolation
PROTOCOL A method for isolating endothelial cells from the mouse brain for cell culture. dselkoe Harvard Medical School and Brigham & Women's Hospital LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-beta peptide in a blood-b
Current Filters
No filters selected