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MDCK Cell Monolayer and Aβ Transport Assay

PROTOCOL This is a protocol for culturing MDCK cells (an epithelial cell line derived from the canine kidney) in a monolayer and assessing Trans-Epithelial Electrical Resistance (TEER) and Aβ transport. dselkoe Harvard Medical School and Brigham & Women's

Immunoprecipitation

PROTOCOL A protocol for enriching for a protein of interest by taking advantage of the specific interaction of an antigen with an antibody. dselkoe Harvard Medical School and Brigham & Women's Hospital IP, Immunoprecipitation protocol_Immunoprecipitation.

Immunohistochemistry (Immunoperoxidase)

PROTOCOL A method for detecting proteins of interest in brain sections using an immunoperoxidase reaction. dselkoe Harvard Medical School and Brigham & Women's Hospital IHC, Immunohistochemistry, brain protocol_Immunohistochemistry.pdf Immunostaining

Aβ ELISA

PROTOCOL A protocol to detect Aβ in tissue culture supernatant and in homogenized tissue. dselkoe Harvard Medical School and Brigham & Women's Hospital Aβ, ELISA, protocol_Abeta_ELISA.pdf ELISA

Coating of Transwell Filters

PROTOCOL A protocol to coat filters with fibronectin and collagen for cell culture. dselkoe Harvard Medical School and Brigham & Women's Hospital LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-beta peptide in a blood-brain

ACE Activity Assay

PROTOCOL A method to measure angiotensin-converting enzyme (ACE) activity. dselkoe Harvard Medical School and Brigham & Women's Hospital An improved fluorometric assay of rat serum and plasma converting enzyme. ACE, angiotensin-converting enzyme, Aβ degra

Collagenase/Dispase Mouse Brain Isolation

PROTOCOL A method for isolating endothelial cells from the mouse brain for cell culture. dselkoe Harvard Medical School and Brigham & Women's Hospital LRP promotes endocytosis and degradation, but not transcytosis, of the amyloid-beta peptide in a blood-b

Coupling Antibodies to Protein A/G

PROTOCOL This protocol can be used to generate an affinity matrix consisting of an antibody covalently linked to Protein A/G-coated beads. dselkoe Harvard Medical School and Brigham & Women's Hospital protein A/G, immunoprecipitation protocol_Coupling_Ant

Culturing Embryonic Fibroblasts

PROTOCOL A protocol for culturing rodent fibroblasts. dselkoe Harvard Medical School and Brigham & Women's Hospital fibroblasts, rodent, cell culture protocol_Culturing_Embryonic_Fibroblasts.pdf Cell Culture

DNA Ethanol Precipitation

PROTOCOL A method for isolating DNA from cells. dselkoe Harvard Medical School and Brigham & Women's Hospital DNA protocol_DNA_ethanol_precipitation.pdf Nucleic Acid Isolation/Purification

Extraction of Human Aβ from APP Tg mouse brains

PROTOCOL Extraction of human Aβ from APP transgenic mouse brain into TBS-soluble, guanidine HCI-soluble fractions. dselkoe Harvard Medical School Aβ, APP, transgenic mice protocol_Extraction_of_human_Abeta_from_APP_Tg_mouse_brain.pdf Protein Isolation/Extraction A

96 Well Aβ ELISA Using Fluorescent Substrate

PROTOCOL A method for detecting Aβ in human samples dselkoe Harvard Medical School and Brigham and Women's Hospital Aβ, ELISA, human protocol_96_Well_Abeta_ELISA.pdf ELISA APP

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