Research Models

GFAP-APOE4/APOE Knock-out

Synonyms: APOE4 (line1)/APOE KO, Tg(GFAP-APOE*4)1Hol

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Species: Mouse
Genes: APOE, APOE
Modification: APOE: Transgenic; APOE: Knock-Out
Disease Relevance: Alzheimer's Disease
Strain Name: B6.Cg-Apoetm1Unc Cdh18Tg(GFAP-APOE_i4)1Hol/J. Formerly: B6.Cg-Tg(GFAP-APOE_i4)1Hol Apoetm1Unc/J
Genetic Background: B6/CBA; back-crossed onto C57BL6 background
Availability: The Jackson Lab: Stock# 004631; Cryopreserved

Summary

These mice express human apolipoprotein E4 (APOE4) driven by the human glial fibrillary acidic protein (GFAP) promoter in the absence of endogenous mouse APOE. Human ApoE4 protein is detectable in glia and neuropil in a pattern that is similar to endogenous murine ApoE expression in wild-type animals. Expression is not observed in neuronal cell bodies. ApoE4 was observed during developmental stages and in mature animals. Secreted ApoE4 was detectable from primary cultures of forebrain astrocytes (Sun et al., 1998).

Modification Details

Transgene of human APOE4 driven by the GFAP promoter; crossed to APOE knock-out mice. The APOE4 transgene inserted at Chr15:23364633-23373281(Build GRCm38/mm10), where it disrupted the Cdh18 gene (Goodwin et al., 2017).

Related Strains

B6.Cg-Tg(GFAP-APOE_i2)14Hol Apoetm1Unc/J
The Jackson Lab, Stock #004632. This line expresses human apolipoprotein E2 (APOE2) driven by the human glial fibrillary acidic protein (GFAP) promoter in the absence of endogenous mouse APOE.

B6.Cg-Tg(GFAP-APOE_i3)37Hol Apoetm1Unc/J
The Jackson Lab, Stock#004633. This line expresses human apolipoprotein E3 (APOE3) driven by the human glial fibrillary acidic protein (GFAP) promoter in the absence of endogenous mouse APOE.

Last Updated: 30 Nov 2018

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References

Paper Citations

  1. Sun Y, Wu S, Bu G, Onifade MK, Patel SN, LaDu MJ, Fagan AM, Holtzman DM. Glial fibrillary acidic protein-apolipoprotein E (apoE) transgenic mice: astrocyte-specific expression and differing biological effects of astrocyte-secreted apoE3 and apoE4 lipoproteins. J Neurosci. 1998 May 1;18(9):3261-72. PubMed.
  2. Goodwin LO, Splinter E, Davis TL, Urban R, He H, Braun RE, Chesler EJ, Kumar V, van Min M, Ndukum J, Philip VM, Reinholdt LG, Svenson K, White JK, Sasner M, Lutz C, Murray SA. Large-scale discovery of mouse transgenic integration sites reveals frequent structural variation and insertional mutagenesis. bioRĪ‡iv preprint first posted online Dec. 18, 2017

External Citations

  1. The Jackson Lab, Stock #004632
  2. The Jackson Lab, Stock#004633
  3. The Jackson Lab: Stock# 004631

Further Reading

No Available Further Reading