Research Models
APOE3 Targeted Replacement
Synonyms: APOE3 Humanized Knock-in
Species: Mouse
Genes: APOE
Modification: APOE: Knock-In
Disease Relevance: Alzheimer's Disease
Strain Name: B6.129P2-Apoetm2(APOE*3)Mae N8
Genetic Background: 129 x C57BL/6; back-crossed to C57BL/6
Availability: Taconic: Stock# 1548-F and 1548-M
Summary
Gene targeting was used to replace the endogenouse murine APOE gene with the human APOE3 allele. On a standard diet, homozygous mice have normal cholesterol and triglyceride levels, but are more susceptible than wild-type animals to diet-induced atherosclerosis (Sullivan et al., 1997); however, they are not as vulnerable as mice with APOE4 Targeted Replacement (Knouff et al., 1999).
Modification Details
Targeted replacement of the endogenous mouse APOE gene with the human APOE3 allele. Targeting vector contained exons 2-4 of human APOE3.
Phenotype Characterization
When visualized, these models will distributed over a 18 month timeline demarcated at the following intervals: 1mo, 3mo, 6mo, 9mo, 12mo, 15mo, 18mo+.
Absent
No Data
- Plaques
- Tangles
- Neuronal Loss
- Gliosis
- Synaptic Loss
- Changes in LTP/LTD
- Cognitive Impairment
Plaques
No data.
Tangles
No data.
Neuronal Loss
No data.
Gliosis
No data.
Synaptic Loss
No data.
Changes in LTP/LTD
No data.
Cognitive Impairment
No data.
Last Updated: 06 Apr 2022
References
Research Models Citations
Paper Citations
- Sullivan PM, Mezdour H, Aratani Y, Knouff C, Najib J, Reddick RL, Quarfordt SH, Maeda N. Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J Biol Chem. 1997 Jul 18;272(29):17972-80. PubMed.
- Knouff C, Hinsdale ME, Mezdour H, Altenburg MK, Watanabe M, Quarfordt SH, Sullivan PM, Maeda N. Apo E structure determines VLDL clearance and atherosclerosis risk in mice. J Clin Invest. 1999 Jun;103(11):1579-86. PubMed.
External Citations
Further Reading
No Available Further Reading
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