Research Models
APOE2 Targeted Replacement
Synonyms: APOE2 Humanized Knock-in
Species: Mouse
Genes: APOE
Modification: APOE: Knock-In
Disease Relevance: Alzheimer's Disease
Strain Name: B6.129P2-Apoetm1(APOE*2)Mae N9
Genetic Background: C57BL/6
Availability: Taconic: Stock# 1547-F and 1547-M
Summary
Targeted gene replacement was used to replace the endogenous murine APOE gene with the human APOE2 allele. In humans, the APOE2 allele is associated with decreased risk of Alzheimer's disease and an increased risk for type III hyperlipoproteinemia. Mice homozygous for the APOE2 allele exhibit characteristics of type III hyperlipoproteinemia. They have plasma cholesterol and triglyceride levels 2-3x higher than levels in mice expressing human APOE3. The APOE2 mice also have deficits in clearing very-low-density lipoprotein (VLDL) particles and spontaneously develop atherosclerotic plaques. A high fat diet exacerbates the atherosclerosis (Sullivan et al., 1998). The human APOE2 protein differs from the most common APOE isoform, APOE3, by a single amino acid at position 158.
Modification Details
Targeted gene replacement of the endogenous murine APOE gene with the human APOE2 allele. Targeting construct included exons 2-4 of APOE2.
Phenotype Characterization
When visualized, these models will distributed over a 18 month timeline demarcated at the following intervals: 1mo, 3mo, 6mo, 9mo, 12mo, 15mo, 18mo+.
Absent
No Data
- Plaques
- Tangles
- Neuronal Loss
- Gliosis
- Synaptic Loss
- Changes in LTP/LTD
- Cognitive Impairment
Plaques
No data.
Tangles
No data.
Neuronal Loss
No data.
Gliosis
No data.
Synaptic Loss
No data.
Changes in LTP/LTD
No data.
Cognitive Impairment
No data.
Last Updated: 29 Aug 2013
References
Paper Citations
- Sullivan PM, Mezdour H, Quarfordt SH, Maeda N. Type III hyperlipoproteinemia and spontaneous atherosclerosis in mice resulting from gene replacement of mouse Apoe with human Apoe*2. J Clin Invest. 1998 Jul 1;102(1):130-5. PubMed.
External Citations
Further Reading
No Available Further Reading
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