Modification: APOE: Knock-Out
Disease Relevance: Alzheimer's Disease
Strain Name: B6.129P2-Apoetm1Unc N11
Genetic Background: 129 x C57BL/6
Availability: Taconic: Stock# APOE-M and APOE-F
Inactivation of the endogenous mouse APOE by homologous recombination and insertion of a neomycin cassette.
ApoE is undetectable in the plasma of these homozygous APOE knock-out mice. They are viable and appear healthy. They have elevated plasma cholesterol compared to wild-type animals (Piedrahita et al., 1992). They develop artherosclerotic lesions which progress to occlusions of coronary artery by eight months (Zhang et al., 1992).
- Piedrahita JA, Zhang SH, Hagaman JR, Oliver PM, Maeda N. Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4471-5. PubMed.
- Zhang SH, Reddick RL, Piedrahita JA, Maeda N. Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science. 1992 Oct 16;258(5081):468-71. PubMed.
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