David Borchelt - Slide #1
Bacterial expression plasmids encoding fusion proteins of glutathione S-transferase (GST) and residues 263-407 of human (Hu) PS1, residues 269-394 of human PS2, and residues 263-407 of mouse PS1 were transformed into bacteria.
- A). Coomassie Blue stained gel of bacterial extracts. The estimated amount (ug) of GST-PS1/PS2 fusion protein is noted above the gel.
- B). Immunoblot analysis of fusion proteins demonstrating the sensitivity of the antibody to sub ng amounts of Hu PS1, lack of cross-reactivity with PS2, and slightly weaker (2.3 fold) reactivity with mouse PS1.
Fig. 1 Specificity and Sensitivity of PS1 Loop antiserum