Updated 3 February 2011

General Information

Targeting of the Tardbp gene by the gene trapping vector pGT1lxf. Confirmed the insertion of the pGT1lxf genetrap cassette within intron 2 of Tardbp at base pair 1226 of ES cell line RB030. The Tardbp^Gt(RB030)Byg chimeric mice were generated in the murine targeted genomics laboratory at the MMRRC from the RB030 ES.

Mouse strain: C57BL/6. Origin: C57BL/6/129. Backcross to both B6 and 129. The Tardbp^Gt(RB030)Byg heterozygous mice were backcrossed to C57BL/6 mice five times. Homozygous are embryonic lethal.

Phenotype

Neuropathological analysis:

Het mice have no evidence of pathologic changes in motor neurons.

Behavioral Phenotype:

Homozygous TDP-43 are embryonic lethal. Het mice exhibit signs of motor disturbance and muscle weakness with decreased forelimb grip strength and deficits in inverted grid test.

Availability

Contact: B.C. Kraemer
Geriatrics Research Education and Clinical Center, Veterans Affairs
Puget Sound Health Care System, Seattle, WA 98108, Phone: 206-277-1071

References

Primary:

Kraemer BC, Schuck T, Wheeler JM, Robinson LC, Trojanowski JQ, Lee VM, Schellenberg GD. Loss of murine TDP-43 disrupts motor function and plays an essential role in embryogenesis. Acta Neuropathol. 2010 Apr;119(4):409-19. Abstract