Posted 1 September 2010

General Information

Genetrap vector inserts β-geo fusion protein into intron 2 of TAR DNA-binding Protein 43 gene. Final product only includes first 65 aa of the native protein fused with βGeo. Results in loss of all functional domains of native protein.

Mutation: N/A

Promoter: N/A

Mouse strain: Origin: 129P2/OlaHsd; Background: C57Bl/6; Generation: F6.

Phenotype

Neuropathological analysis:

LacZ staining of het E9.5 tissues shows expression in neuroepithelium and in all neural progenitors from E10.5-12.5. By E12.5 expression seen in spinal cord progenitors, differentiated motor neurons, and in the dorsal root ganglia. Adult Tardbp^+/- tissues show widespread expression in various regions of the CNS.

Behavioral Phenotype:

Tardbp^-/- are embryonic lethal between E3.5 and E8.5. Tardbp^+/- mice are viable, fertile, normal in size and display no overt phenotype.

Availability

Contact: Gang Yu
Dept. of Neuroscience
University of Texas Southwestern Medical Center
6000 Harry Hines Blvd.
Dallas, Texas 75390-9111
Telephone: 214-648-5157;
Fax: 214-648-1801
E-mail: Gang.Yu@UTSouthwestern.edu

References

Primary:

Sephton CF, Good SK, Atkin S, Dewey CM, Mayer III P, Herz J, Yu G. TDP-43 is a developmentally regulated protein essential for early embryonic development. J Biol Chem 2010 285(9), 6826. Abstract