Posted 1 September 2010
Genetrap vector inserts β-geo fusion protein into intron 2 of TAR DNA-binding
Protein 43 gene. Final product only includes first 65 aa of the native protein fused
with βGeo. Results in loss of all functional domains of native protein.
Mouse strain: Origin: 129P2/OlaHsd; Background: C57Bl/6; Generation: F6.
LacZ staining of het E9.5 tissues shows expression in neuroepithelium and in all
neural progenitors from E10.5-12.5. By E12.5 expression seen in spinal cord progenitors,
differentiated motor neurons, and in the dorsal root ganglia. Adult Tardbp+/-
tissues show widespread expression in various regions of the CNS.
Tardbp-/- are embryonic lethal between E3.5 and E8.5. Tardbp+/-
mice are viable, fertile, normal in size and display no overt phenotype.
Contact: Gang Yu
Dept. of Neuroscience
University of Texas Southwestern Medical Center
6000 Harry Hines Blvd.
Dallas, Texas 75390-9111
Sephton CF, Good SK, Atkin S, Dewey CM, Mayer III P, Herz J, Yu G. TDP-43 is a developmentally
regulated protein essential for early embryonic development. J Biol Chem 2010 285(9),