Posted 10 June 2005
Transgene: Mutant tau-P301L was obtained by site-directed mutagenesis of tau-4R/2N
and cloned into the blunted XhoI site of the thy1 gene expression cassette. The
construct was linearized with NotI and PvuI and injected into prenuclear mouse embryos
(0.5-day) of the FVB/N strain.
Mutation: Human Tau-P301L in the Tau-4R/2N isoform
Promoter: mouse Thy1
Mouse strain: FVB/N
Expression level, similar to Tau-4R/2N. Axonal dilations, absent. Intra-neuronal
tau aggregates (NFT), progressive > 6 months, severe at 9-12 months.
Clasping, impaired. Rotarod, normalBeam walk, failed >9 months. Mean life span <12
Licensing/academic distribution contact information:
Paul Van Dun
Director - KULeuvenR&D
Groot Begijnhof 59
B-3000 Leuven Belgium
tel +32 16 326508
fax +32 16 326515
Web site: http://www.kuleuven.ac.be/lrd
Terwel D, Lasrado R, Snauwaert J, Vandeweert E, Van Haesendonck C, Borghgraef P,
Van Leuven F.Changed conformation of mutant tau-P301L underlies the moribund tauopathy,
absent in progressive, non-lethal axonopathy of tau-4R/2N transgenic mice. J Biol
Chem (2005) 280: 3963-3973.