Posted 6 March 2005
Transgene: cDNA construct containing the longest human brain tau isoform (T40) (hWT
tau) was cloned into the mouse prion promoter (MoPrP.Xho) expression vector at the
XhoI site. A 15.3 kb NotI fragment containing T40, the MoPrP promoter, together
with its 3' and 5' untranslated sequences, was microinjected into fertilized C57BL6
x C3H (B6C3/F1) mouse eggs and then implanted into pseudopregnant females. Two lines
of hWT tau Tg mice (lines 1 and 23) that stably expressed the T40 human tau isoform
were identified. Most experiments performed on line 23.
Mutation: human WT tau mutation
Promoter: Mouse prion protein promoter (MoPrP)
Mouse Strain: B6C3
Human Tau expression is 8-10 times higher than endogenous tau in line 23. No age-dependent
accumulation of tau aggregates, no neurodegeneration.
90% survive to 24 months
Contact: Virginia M-Y Lee
The Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory
Institute on Aging, University of Pennsylvania School of Medicine, Philadelphia,
Zhang B, Higuchi M, Yoshiyama Y, Ishihara T, Forman M S, Martinez D, Joyce S, Trojanowski
J Q, Lee V M-Y. Retarded Axonal Transport of R406W Mutant Tau in Transgenic Mice
with a Neurodegenerative Tauopathy. J Neurosci. 24(19):4657-4667, 2004.