General Information

Generation of PS2 KO mice: Mice carrying an ablated PS2 gene were created by targeting exon 5 of the mouse gene in ES cells. A small deletion in exon 5 and insertion of the neomycin resistance gene was sufficient to disrupt translation.

F1 mice derived from a cross between the targeted line (129sv/ev) and C57/blk6. 

Phenotype

Neuropathological analysis:

Gross external examination of neonates and adult null showed that PS2-ablated mice develop normally up to the oldest age studied (1 year).

Gross analysis of brain cytoarchitecture by Nissl stain showed that ablated and wt mice have similar neuroanatomy.

No accumulation of APP CTFs and no change in Ab production.

Behavioral:

The lack of PS2 gene in mice does not result in an obvious phenotype. 

Availability

Licensing/academic distribution contact information:

Patents: None

Reference

Primary:

Steiner H, Duff K, Capell A, Romig H, Grim MG, Lincoln S, Hardy J, Yu X, Picciano M, Fechteler K, Citron M, Kopan R, Pesold B, Keck S, Baader M, Tomita T, Iwatsubo T, Baumeister R, Haass C.  A loss of function mutation of presenilin-2 interferes with amyloid beta-peptide production and notch signaling. J Biol Chem 1999 Oct 1;274(40):28669-73.  Abstract.