General Information

Transgene: The pNSE-hPS2w and pNSE-hPS2m were constructed by combining the NSE promoter containing SacI-SphI fragment from pNSE-CAT and the hPS2m (N141I, Volga German Families). Each SacII/XbaI fragment of hPS2w and hPS2m was inserted in pUHD10-3. The pNSE-CAT and pUHD10-3 were gifts of Dr. J. Gregor Sutcliffe and Dr. Tae-Wan Kim, respectively. Each linear NSE-PS2w and NSE-PS2m fragment was microinjected into pronucleus of fertilized BDF1mice eggs then transferred to the oviducts of pseudopregnant ICR recipient females.

Mutation: N141I

Promoter: NSE promoter

Strain: C57BL/6× DBA/2

Phenotype

Neuropathological analysis:

Levels of hPS2, Aβ-42, caspase-3, and Cox-2 expression were modulated in the brains of both wt and tg mice. Dense staining with antibody to hPS2, Aβ-42, caspase-3, and Cox-2 was visible in the brains of tg mice compared with age-matched controls, and distinguishable AD phenotypes between hPS2w- and hPS2m-Tg mice did not appear.

Behavioral:

Tg mice showed a behavioral dysfunction in the water maze test.

Availability

Yong K. Kim
Division of Laboratory Animal Resources
Korea FDA, National Institute of Toxicological Research
5 Nokbun-dong Eunpyng-ku
Seoul 122-704, Korea
Telephone: +82-2-380-1835
Fax: +82-2-380-1833
Email: kimyongkyu@hanmail.net

Patents: PS2-transgenic mice; Jung S. Cho / Yong K. Kim et al.; Filed 11/4/02

Reference

Primary:

Hwang DY, Chae KR, Kang TS, Hwang JH, Lim CH, Kang HK, Goo JS, Lee MR, Lim HJ, Min SH, Cho JY, Hong JT, Song CW, Paik SG, Cho JS, Kim YK. Alterations in behavior, amyloid beta-42, caspase-3, and Cox-2 in mutant PS2 transgenic mouse model of Alzheimer's disease. FASEB J. 2002 Jun;16(8):805-13. Abstract.