Posted 30 May 2006

General Information

Transgene: A 2.3 kb cDNA fragment containing the entire coding region of the (h)SIRT1 was inserted into a unique HindIII-Not I restriction site located within the second exon of the rat neuron-specific enolase (rNSE) gene.Promoter: rat neuron specific enolase (NSE) promoter, including 2600 bp segment of promoter and 5' flanking region, as well as exon 1, intron 1, and 6 bp of exon 2 of rat NSE gene.

Mutation: None.

Promoter: Rat NSE

Mouse strain: C57Bl/6J x SJL/J (B6SJL) F1

Phenotype

Neuropathological Analysis:

~ 2 fold elevation of hSIRT1 expression was found in cortex of ~ 3 months-old (h)SIRT1, relative to gender-matched wild type (WT) littermate controls. A significant diminution of ROCK1 protein content (p<0.05) in the same brain tissue was also found, relative to gender-matched wild type (WT) littermate controls. We also detected a commensurate elevation of a-secretase activity (P<0.05) in the cortex of (h) SIRT1 trangenics, relative to gender-matched wild type (WT) littermate controls.

Behavioral

N/A

Availability

Giulio Maria Pasinetti
Neuroinflammation Research Laboratories, Department of Psychiatry
Mount Sinai School of Medicine, One Gustave L. Levy Place
New York, NY 10029, USA
Phone: +1-212-241-5579
Fax: +1-212-876-9042
Email: giulio.pasinetti@mssm.edu

References

Primary:

Qin W. , Yang T. , Ho L., Zhao Z. , Wang J. , Chen L. , Thiyagarajan M. , MacGrogan D., Rodgers J.T. , Puigserver P., Sadoshima J. , Deng H., Pedrini S., Gandy S., Sauve A., and Pasinetti G.M. Neuronal SIRT1 activation as a novel mechanism underlying the prevention of Alzheimer's disease amyloid neuropathology by calorie restriction. Submitted.