Posted 30 May 2006
Transgene: A 2.3 kb cDNA fragment containing the entire coding region of the (h)SIRT1
was inserted into a unique HindIII-Not I restriction site located within the second
exon of the rat neuron-specific enolase (rNSE) gene.Promoter: rat neuron specific
enolase (NSE) promoter, including 2600 bp segment of promoter and 5' flanking region,
as well as exon 1, intron 1, and 6 bp of exon 2 of rat NSE gene.
Promoter: Rat NSE
Mouse strain: C57Bl/6J x SJL/J (B6SJL) F1
~ 2 fold elevation of hSIRT1 expression was found in cortex of ~ 3 months-old (h)SIRT1,
relative to gender-matched wild type (WT) littermate controls. A significant diminution
of ROCK1 protein content (p<0.05) in the same brain tissue was also found, relative
to gender-matched wild type (WT) littermate controls. We also detected a commensurate
elevation of a-secretase activity (P<0.05) in the cortex of (h) SIRT1 trangenics,
relative to gender-matched wild type (WT) littermate controls.
Giulio Maria Pasinetti
Neuroinflammation Research Laboratories, Department of Psychiatry
Mount Sinai School of Medicine, One Gustave L. Levy Place
New York, NY 10029, USA
Qin W. , Yang T. , Ho L., Zhao Z. , Wang J. , Chen L. , Thiyagarajan M. , MacGrogan
D., Rodgers J.T. , Puigserver P., Sadoshima J. , Deng H., Pedrini S., Gandy S.,
Sauve A., and Pasinetti G.M. Neuronal SIRT1 activation as a novel mechanism underlying
the prevention of Alzheimer's disease amyloid neuropathology by calorie restriction.