Posted 13 June 2005

General Information

Transgene: The targeting construct contained a genomic 7.5-kb SstI/EcoRI fragment comprising exons 16-19 of the MAM gene with a positive selection marker gene, hygromycin B phosphotransferase with the PGK promotor, embedded in intron 17. Transfection into E14 ES cells. ES cell lines were injected into C57Bl blastocysts and implanted into (C57Bl female; x CBA/J male;) foster mothers.

Promoter: PGK promoter

Mouse strain: C57Bl

Phenotype

Behavioral:

No phenotypic abnormalities

Availability

Licensing/academic distribution contact information:

Paul Van Dun
Director - KULeuvenR&D
Groot Begijnhof 59
B-3000 Leuven Belgium
tel +32 16 326508
fax +32 16 326515
Email: Paul.Vandun@lrd.kuleuven.ac.be
Web site: http://www.kuleuven.ac.be/lrd

Patents: none

References

Primary:

Umans L, Serneels L,Overbergh L, Lorent K, Van Leuven F, Van den Berghe H. Targeted Inactivation of the Mouse a2-Macroglobulin Gene. J Biol Chem 1995, 270:19778-1978. Abstract.

Associated:

Umans L, Serneels L, Overbergh L, Stas L, Van Leuven F. Alpha2-macroglobulin- and murinoglobulin-1- deficient mice. A mouse model for acute pancreatitis. Am J Pathol. 1999 155(3):983-93. Abstract

Lorent K, Overbergh L, Moechars D, De Strooper B, Van Leuven F, Van den Berghe H. Expression in mouse embryos and in adult mouse brain of three members of the amyloid precursor protein family, of the alpha-2-macroglobulin receptor/low density lipoprotein receptor-related protein and of its ligands apolipoprotein E, lipoprotein lipase, alpha-2-macroglobulin and the 40,000 molecular weight receptor-associated protein. Neuroscience. 1995 Apr; 65(4): 1009-25. Abstract

Rogaeva EA, Premkumar S, Grubber J, Serneels L, Scott WK, Kawarai T, Song Y, Hill DM, Abou-Donia SM, Martin ER, Vance JJ, Yu G, Orlacchio A, Pei Y, Nishimura M, Supala A, Roberge B, Saunders AM, Roses AD, Schmechel D, Crane-Gatherum A, Sorbi S, Bruni A, Small GW, Conneally PM, Haines JL, Van Leuven F, St. George-Hyslop PH, Farrer LA, Pericak-Vance MA. An a-2-macroglobulin insertion-deletion polymorphism in Alzheimer disease. Nat Genet. 1999 May; 22(1): 19-22. Abstract

Hochepied T, Van Leuven F, Libert C.Mice lacking alpha(2)-macroglobulin show an increased host defense against Gram-negative bacterial sepsis, but are more susceptible to endotoxic shock. Eur Cytokine Netw. 2002 Jan-Mar;13(1):86-91. Abstract

Esadeg S, He H, Pijnenborg R, Van Leuven F, Croy BA. Alpha-2 macroglobulin controls trophoblast positioning in mouse implantation sites.Placenta. 2003 Nov;24(10):912-21. Abstract

He H, McCartney DJ, Wei Q, Esadeg S, Zhang J, Foster RA, Hayes MA, Tayade C, Van Leuven F, Croy BA. Characterization of a murine alpha 2 macroglobulin gene expressed in reproductive and cardiovascular tissue. Biol Reprod. 2005 Feb;72(2):266-75. Abstract