Posted 10 August 2004

General Information

Transgene: The BACE54 construct was obtained by insertion of the complete hBACE cDNA into the filled-in XhoI site of the vector pTSC α1.

Mutation: hBACE

Promoter:  murine Thy-1

Mouse strain: C57BL/6 J background (gen >10)

Phenotype

Neuropathological analysis

Decreased levels of full-length mature APP and increased levels of C99 and C89. Human BACE mRNA 4x higher than endogenous. Highest expression of hBACE protein in cortex and hippocampus, lowest in cerebellum.

Behavioral

No major changes.

Availability

Licensing/academic distribution contact information:

Paolo Paganetti
Neuroscience Research
Novartis Pharma AG
P.O. Box WSJ-386.8.28, CH-4002
Basel, Switzerland
Email: paolo.paganetti@pharma.novartis.com

Patents: None

References

Primary:

Bodendorf U, Danner S, Fischer F, Stefani M, Sturchler-Pierrat C, Wiederhold K-H, Staufenbiel M, Paganetti P. Expression of human β-secretase in the mouse brain increases the steady-state level of β-amyloid. J Neurochem. 80:799, 2002. Abstract

Associated:

Pastorino L, Ikin AF, Lambrianou S, Vacaresse N, Revelli JP, Platt K, Paganetti P, Mathews PM, Harroch S and JD Buxbaum. 2004. BACE ( β-secretase) modulates the processing of APLP2 in vivo. Mol. Cell. Neurosci. 25: 642-649. Abstract