Posted 10 June 2005

General Information

Transgene: The cDNA's coding for C-terminally HA-tagged bovine ADAM10 (a disintegrin and metalloproteinase) dominant-negative form, ADAM10-E384A-HA (ADAM10-dn), was cloned under control of a neuron-specific mouse thy1 promoter. The minigene was microinjected into pronuclear embryos from superovulated FVB/N females.

Promoter: Mouse Thy1

Mouse strain: FVB/N

Phenotype

Neuropathological Analysis:

The inactive form of ADAM10 has the point mutation E384A in the zinc-binding motif. In cultured cells, HA-tagged ADAM10 E384A mutant has been shown to inhibit generation of APPsα.

Behavioral

normal

Availability

Not available. For information on these mice, please contact:
Falk Fahrenholz
Institute of Biochemistry
University of Mainz
Becherweg 30, D-55099 Mainz, Germany
Fax: 49-6131-392-5348
Email: bio.chemie@uni-mainz.de

References

Primary:

Postina R, Schroeder A, Dewachter I, Bohl J, Schmitt U, Kojro E, Prinzen C, Endres K, Hiemke C, Blessing M, Flamez P, Dequenne A, Godaux E, van Leuven F, Fahrenholz F. A disintegrin-metalloproteinase prevents amyloid plaque formation and hippocampal defects in an Alzheimer disease mouse model. J. Clin. Invest. 113: 1456-1464, 2004. Abstract