Posted 6 March 2005

General Information

Transgene: To generate bigenic mice, APPSw mice Tg2576 (see TAC #001349) were crossed with BACE Tg lines to generate double heterozygous offspring.

Mutation: hBACE, APPSw(KM670/671NL)

Promoter: Mouse prion promoter (moPrP)

Mouse Strain: C57BL/6/C3H/SJL

Phenotype

Neuropathological Analysis:

BACE expression was similar in single BACE and bigenic APPxBACE mice. In bigenic mice high BACE overexpression inhibited amyloid formation despite increased ß-cleavage of APP. Also the high BACE expression shifted the subcellular location of APP cleavage to the neuronal perikarya early in the secretory pathway thereby depleting APP destined for axonal transport.

Behavioral:

N/A

Availability

Contact:Virginia M-Y Lee
The Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine
Institute on Aging, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Phone: 215-662-6427
Fax: 215-349-5909
Email: vmylee@mail.med.upenn.edu

Patents: None

References

Primary:

Lee EB, Zhang B, Liu K, Greenbaum EA, Doms RW, Trojanowski JQ, LeeVM-Y. BACE overexpression alters the subcellular processing of APP and inhibits Aβ deposition in vivo J. Cell Biol. 168: 291 - 302, 2005. Abstract.