Posted 10 June 2005

General Information

Transgene: APPV717I crossed with BACE-1 (Line 16)

Mutation: HBACE-1 and APP(London, V717I)

Promoter: Mouse Thy1 (both transgenes)

Mouse Strain: Offspring in F1 generation are double-transgenic mice that are heterozygous for both transgenes and with identical F1(FVB x C57Bl) genetic background.

Phenotype

Neuropathological Analysis:

Neuronal co-expression of BACE and mutant APP-V717I. In brain of double tg mice, amyloidogenic processing is increased at both Asp1 and Glu11 resulting in more Aβ peptides, including more N-truncated Aβ peptides. Pathologically, BACE1 increased the number of diffuse and senile amyloid plaques in older bi-genic mice but reduced the vascular amyloid relative to that in single APPV717I tg mice at the same age.

Availability

Licensing/academic distribution contact information:

Paul Van Dun
Director - KULeuvenR&D
Groot Begijnhof 59
B-3000 Leuven Belgium
tel +32 16 326508
fax +32 16 326515
Email: Paul.Vandun@lrd.kuleuven.ac.be
Web site: http://www.kuleuven.ac.be/lrd

Patents: None

References

Primary:

Willem M, Dewachter I, Smyth N, Van Dooren T, Borghgraef P, Haass C, Van Leuven F. β-site amyloid precursor protein cleaving enzyme 1 increases amyloid deposition in brain parenchyma but reduces cerebrovascular amyloid angiopathy in aging BACE x APP (V717I) double-transgenic mice. Am J Path. 105(5): 1621-1631, 2004. ">Abstract.

Associated:

Moechars D, Dewachter I, Lorent K, Reverse D, Baekelandt V, Naldu A, Tesseur I, Spittaels K, Van Den Haute C, Checkler F, Godaux E, Cordell B, Van Leuven F. Early phenotypic changes in transgenic mice that overexpress different mutants of Amyloid Precursor Protein in brain. J. Biol. Chem. (1999) 274: 6483-6492. Abstract.