Posted 1 April 2005

General Information

Transgene: Transgene bearing dominate negative RAGE (DN-RAGE) driven by the platelet-derived growth factor (PDGF) ß-chain promoter was constructed and subcloned into the 6apc1 vector and injected into mouse B6CBAF1/J oocytes and then implanted into pseudopregnant females that were subsequently mated with B6CBAF1/J males. The mice were termed DN-RAGE. These mice were crossed with APP (Tg mAPP) also driven by PDGF β-chain promoter, and in the C57BL/6 background (see TJL #004661).

Promoter: PDGF-B promoter for both APP and DN-RAGE

Mouse strain: C57BL/6

Phenotype

Neuropathological analysis

Tg mice bearing a dominant-negative RAGE construct targeted to neurons crossed with mAPP animals displayed preservation of spatial learning/memory and diminished neuropathologic changes.

Behavioral

Tg DN-RAGE animals did not manifest abnormalities with respect to reproductive fitness, development, basic neurologic function or longevity.

Availability

Shi Du Yan
Departments of Pathology and Surgery, Departments of Pathology and Surgery
Taub Institute for Alzheimer's Disease and the Aging Brain
College of Physicians & Surgeons of Columbia University
630 West 168th Street
New York, NY 10032, USA.
Phone: (212) 305 3958
Fax: (212) 305 5337
Email: sdy1@columbia.edu

Patents: None

References

Primary:

Arancio O, Zhang HP, Chen X, Lin C, Trinchese F, Puzzo D, Liu S, Hegde A, Yan SF, Stern A, Luddy JS, Lue L-F, Walker DG, Roher A, Buttini M, Mucke L, Li W, Schmidt AM, Kindy M, Hyslop PA, Stern DM, Yan SSD. RAGE potentiates Aβ-induced perturbation of neuronal function in transgenic mice. The EMBO J. 23 (20): 4096-4105, 2004. Abstract