General Information

Transgene: The coding sequence for human mutant [A30P] αSYN was cloned into a cassette containing 4.5-kb 5' flanking DNA from the rat TH gene using NheI and SpeI. The linearized construct was injected into fertilized DBA X C57BL/6 eggs.

Promoter: Rat TH promotor

Mouse Strain: Backcrossed into the C57BL/6 strain for 4 generations (2001)

Phenotype

Neuropathological Analysis:

Tg mice showed somal and neuritic accumulation of transgenic [A30P] αSYN in TH-positive neurones in the substantia nigra. However, there was no difference in the number of TH-positive neurones in the substantia nigra and the concentrations of catecholamines in the striatum between these transgenic mice and non-transgenic littermates.

Behavioral:

No motor disability

Availability

Philipp Kahle
Lab of Alzheimer's and Parkinson's Disease Res, Dept Biochemisty
Ludwig Maximilians University of Munich
Schillerstrasse 44, 80336, Munich, Germany
(+49-89) 5996-480, 475
(+49-89) 5996-415
Email: pkahle@pbm.med.uni-muenchen.de

Patents:

References

Primary:

Rathke-Hartlieb S, Kahle PJ, Neumann M, Ozmen L, Haid S, Okochi M, Haass C, Schulz JB. Sensitivity to MPTP is not increased in Parkinson's disease-associated mutant alpha-synuclein transgenic mice. J Neurochem. 2001 May 1 ; 77(4):1181-4. Abstract