Transgene: The coding sequence for human mutant [A30P] αSYN was cloned into a cassette containing 4.5-kb 5' flanking DNA from the rat TH gene using NheI and SpeI. The linearized construct was injected into fertilized DBA X C57BL/6 eggs.
Promoter: Rat TH promotor
Mouse Strain: Backcrossed into the C57BL/6 strain for 4 generations (2001)
Neuropathological Analysis:
Tg mice showed somal and neuritic accumulation of transgenic [A30P] αSYN in TH-positive neurones in the substantia nigra. However, there was no difference in the number of TH-positive neurones in the substantia nigra and the concentrations of catecholamines in the striatum between these transgenic mice and non-transgenic littermates.
Behavioral:
No motor disability
Philipp Kahle
Lab of Alzheimer's and Parkinson's Disease Res, Dept Biochemisty
Ludwig Maximilians University of Munich
Schillerstrasse 44, 80336, Munich, Germany
(+49-89) 5996-480, 475
(+49-89) 5996-415
Email: pkahle@pbm.med.uni-muenchen.de
Patents:
Primary:
Rathke-Hartlieb S, Kahle PJ, Neumann M, Ozmen L, Haid S, Okochi M, Haass C, Schulz JB. Sensitivity to MPTP is not increased in Parkinson's disease-associated mutant alpha-synuclein transgenic mice. J Neurochem. 2001 May 1 ; 77(4):1181-4. Abstract
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