Transgene: Human APP695 cDNA
Mutation: APP695 with double mutations
Promoter: hamster prion protein gene promoter
Mouse Strain: C57B6j X SJL F1 hybrid mice backcrossed N1 or N2 generations
Brain transgenic APP was 5.6 times more than endogenous APP. A fivefold increase
in Ab(1-40) and a 14 fold increase in Ab(1-42/43)
over levels measure in young, unimpaired transgenic mice.
Numerous Ab plaques stained with Congo red dye in cortical
and limbic structures of mice with elevated amount of Ab.
Plaque load in these mice ranged from 3.6 to 8.5% in selected cellular inflammatory
response. Amyloid angiopathy appeared in some vessels. Markers to oxidative lipid
and glycoxidative damages as well as to the antioxidant defense enzymes heme-oxygenase
and superoxide dismutase were increased in transgenic animals.
Neither neuronal loss in the CA1 region nor loss of synaptic density in the dentate
gyrus of hippocampi in aged mice was detected.
Normal spatial reference memory in N2 mice at 3 months. In a forced alternation
paradigm and spatial reference memory in a water maze were impaired in N2 mice at
9-10 months N1 mice: spatial reference impairment.
Taconic Farm Emerging Model
Transgenic mice expressing APP-Swedish mutation develop progressive neurologic disease
Johns Hopkins University; Regents of the University of Minnesota /Hsiao; Karen;
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