Updated 9 December 2008
Transgene: A transgenic construct of a chimeric mo/hu amyloid precursor protein
(APPswe/ind) under the control of a tetracycline-responsive promoter element (TRE
or tetO) was injected into fertilized C57BL/6J X C3HeJ F2 mouse eggs. Founder line
8-85 was established. Male hemizygous mice were mated to female B6C3F1/J (Stock
No. 100010) mice. Generation: [?+N1p]+N4 (11-Nov-08).
Transgenic APP expression was activated by crossing the APPswe/ind mice to animals
producing tTA under control of the CaMKIIα promoter (see Stock No. 003010 for
example). Nearly complete suppression of the transgene can be shown by treatment
Mutation: Swedish (KM570/571NL) and Indiana (V617F)
Promoter: Tetracycline-responsive promoter element (TRE or tetO)
Breeding: TJL mating systerm: Hemizygote x F1 (Female x Male)
Tg mice express a chimeric mo/huAPP protein at 10-30 fold over endogenous levels.
When bred with another tg mouse expressing reverse tet-controlled transactivator
protein (reTA) or tetracycline-controlled transactivator protein (tTA) the APPSwe/Ind
tg expression can be regulated with doxycycline.
Doxycycline treatment inhibits APP expression by >95% and reduces Aβ production
to levels found in non-tg mice.
Plaques in forebrain at 6 months.
Hemizygous mice are viable and fertile.
Bigenic mice were 10-fold more active during dark phase of day-night cycle.
Hyperactivity was abolished by rearing bigenic mice on doxycycline.
Terms and Conditions: This strain is not available to companies or For Profit-Entities;
it is available to research institutions for non-commercial research.
Use of the Tet-System may require a license; see Licenses for Strains Using TET-System Technology.
Contact: The Jackson
Lab, available, Stock # 006004.
Jankowsky JL, Slunt HH, Gonzales V, Savonenko AV, Wen JC, Jenkins NA, Copeland NG,
Younkin LH, Lester HA, Younkin SG, Borchelt DR. Persistent amyloidosis following
suppression of Abeta production in a transgenic model of Alzheimer disease. PLoS
Med. 2005 Dec;2(12):e355. Epub 2005 Nov 15
Borchelt DR; Davis J; Fischer M; Lee MK; Slunt HH; Ratovitsky T; Regard J; Copeland
NG; Jenkins NA; Sisodia SS; Price DL. 1996. A vector for expressing foreign genes
in the brains and hearts of transgenic mice. Genet Anal 13(6):159-63.