Transgene: Human βAPP cDNA carboxy terminal 99 a.a. and βAPP signal peptide
(without AD-specific mutations)
Promoter: cytomegalovirus enhancer/chicken β-actin
Mouse Strain: B6C3F1 x C57BL/6N hybrid
Accumulation of human Aβ at very high level in pancreas (8600 pmol/g) and in
lesser, though readily detectable, amounts in brain (4.1 pmol/g) and kidney (15.0
Little correlation between Aβ accumulation and the expression of transgenic
mRNA because Aβ was not detected in tissues such liver and heart which expressed
more transgenic mRNA than pancreas, brain, or kidney.
Accumulation of human Aβ in transgenic plasma in an amount over 30 times that
in normal human plasma.
Putative amyloid fibrils in pancreas both in acinar cells and in interstitial macrophages.
Like amyloid fibrils in the AD brain, these fibrils had a diameter of 7-12 nm and
were stained by anti-Aβ antibody.
Kawarabayashi T, Shoji M, Sato M, Sasaki A, Ho L, Eckman CB, Prada C-M, Younkin
SG, Kobayashi T, Norihiro T, Matsubara E, Iizuka T, Harigaya Y, Kasai K, Hirai S.
Accumulation of β-amyloid fibrils in pancreas of transgenic mice. Neurobiol
Shoji M, Kawarabayashi T, Sato M, Sasaki A, Saido TC, Matsubara E, Tomidokoro Y,
Kanai M, Shizuka M, Ishiguro K, Ikeda M, Harigaya Y, Okamoto K, Hirai S. Age-related
amyloid β protein accumulation induces cellular death and macrophage activation
in transgenic mice. J Pathol 2000 May;191(1):93-101.
Shoji M, Kawarabayashi T, Sato M, Sasaki A, Matsubara E, Igeta Y, Kanai M, Tomidokoro
Y, Shizuka M, Ishiguro K, Harigaya Y, Okamoto K, Hirai S. Accumulation of amyloid
β protein in transgenic mice. Neurobiol Aging 1998 Jan-Feb;19(1 Suppl):S59-63.