Posted 13 June 2005

General Information

Transgene: The construct contained the human BACE cDNA inserted in the mouse thy-1 gene cassette at the XhoI site of the pTSC plasmid. Final constructs were linearized, purified and microinjected into pronuclear DBA/C57BL6 embryos.

Mutation: huBACE (wt)

Promoter: Mouse Thy1

Mouse Strain: The five resulting BACE transgenic founders were expanded into the C57BL background.

Phenotype

Neuropathological Analysis:

Transgene expressed in neurons only. Line 16 had high expression of human BACE.

Behavioral:

N/A

Availability

Michael Willem
Department of Biochemistry
Laboratory for Alzheimer's and Parkinson's Disease Research
Ludwig-Maximilians-University
Schillerstr. 44, 80336 Munich
Phone: 089 - 2180 75 - 471
Fax: 089 - 2180 75 - 415
Email: michael.willem@med.uni-muenchen.de
Note: Embryos are stored at Charles River Breeding Labs.

Patents: None

References

Primary:

Willem M, Dewachter I, Smyth N, Van Dooren T, Borghgraef P, Haass C, Van Leuven F. b-site amyloid precursor protein cleaving enzyme 1 increases amyloid deposition in brain parenchyma but reduces cerebrovascular amyloid angiopathy in aging BACE x APP(V717I) double-transgenic mice. Am J Path. 105(5): 1621-1631, 2004. Abstract.