Posted 6 March 2005

General Information

Transgene: The hBACE cDNA was cloned into the MoPrP.Xho expression vector at the XhoI restriction site to generate a 15.9-kb NotI linear fragment. DNA was microinjected into C57B6/C3H mouse.

Mutation: hBACE

Promoter: Mouse prion protein (PrP) promoter

Mouse Strain: C57B6/C3H

Phenotype

Neuropathological Analysis:

Produced three tg lines BACE-L (line 30), BACE-M (line 34), and BACE-H (line 8), expressing BACE ~7-, 10-, and 20-fold over endogenous levels, respectively. BACE expression was similar in single BACE and bigenic APPxBACE mice. Monogenic BACE mice showed no evidence of Aß deposition. Immunostaining showed increased BACE expression in neuronal cell bodies, axons and synaptic elements such as the mossy fiber terminals of the hippocampus, puncta within the granule cell layer of the cerebellum, and neuropil within the spinal cord.

Behavioral:

N/A

Availability

Contact:Virginia M-Y Lee
The Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine
Institute on Aging, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
Phone: 215-662-6427
Fax: 215-349-5909
Email: vmylee@mail.med.upenn.edu

Patents: None

References

Primary:

Lee EB, Zhang B, Liu K, Greenbaum EA, Doms RW, Trojanowski JQ, LeeVM-Y. BACE overexpression alters the subcellular processing of APP and inhibits Aβ deposition in vivo J. Cell Biol. 168: 291 - 302, 2005. Abstract.