Posted 2 October 2004
Tg mice overexpress the signal plus 99-amino acid carboxyl-terminal sequence (SbC)
of β-APP under the control of a cytomegalovirus enhancer/β-actin promoter.
Mutation: C99 of β-APP (5 copies)
Promoter: α cytomegalovirus enhancer/β-actin promoter
Mouse Strain: The transgene is injected into fertilized eggs produced by mating
between F1 of C57BL/6 x DBA/2. C57BL/6J generations N6 to N8. More than 10 generations
(>N10). The transgenic male mice are crossed to C57BL/6 female mice. The litter
size is 10-12 on average.
Tg mice developed Aβ-immunoreactive deposits within the skeletal muscle fibers
after 6 to 12 months of age. The muscle fibers with Aβ-immunoreactive deposits
increased and became vacuolated with age.
Mice exhibit hypoactivity and spatial learning deficit associated with higher cytochrome
oxidase activity seen in thalamic nuclei.
Dr. Ken-ichiro Fukuchi
Department of Comparative Medicine
Tg13592 Schools of Medicine and Dentistry, University of Alabama at Birmingham,
Kaul 640B, 720 20th Street South,
Birmingham, AL 35294-0019.
Fukuchi K, Ho L, Younkin SG, Kunkel DD, Ogburn CE, Wegiel J, Wisniewski HM, LeBoeuf
RC, Furlong CE, Deeb SS, Nochlin D, Sumi SM, Martin GM: High levels of β-amyloid
protein in peripheral blood do not cause cerebral β-amyloidosis in transgenic
mice. Am J Pathol 149:219-227, 1996.
Fukuchi K., Pham D., Hart M., Li L., Lindsey J.R. ß-Amyloid protein deposition in
skeletal muscle of transgenic mice: Possible modes of inclusion body myopathy. Am.
J. Pathol. 153, 1687 1693, 1998.
Fukuchi K, Li L, Hart M, Lindsey JR. Accumulation of amyloid-beta protein in exocrine
glands of transgenic mice overexpressing a carboxyl terminal portion of amyloid
protein precursor. Int J Exp Pathol. 81(4):231-9, 2000.
Lalonde R, Dumont M, Fukuchi K, Strazielle C. Transgenic mice expressing the human
C99 terminal fragment of betaAPP: effects on spatial learning, exploration, anxiety,
and motor coordination. Exp Gerontol., 37(12):1401-12, 2002.
Strazielle C, Dumont M, Fukuchi K, Lalonde R. Transgenic mice expressing the human
C99 terminal fragment of betaAPP: effects on cytochrome oxidase activity in skeletal
muscle and brain. J Chem Neuroanat. 27(4): 237-46, 2004.