General Information

Posted 2 October 2004

Transgene:

Tg mice overexpress the signal plus 99-amino acid carboxyl-terminal sequence (SbC) of β-APP under the control of a cytomegalovirus enhancer/β-actin promoter.

Mutation: C99 of β-APP (5 copies)

Promoter: α cytomegalovirus enhancer/β-actin promoter

Mouse Strain: The transgene is injected into fertilized eggs produced by mating between F1 of C57BL/6 x DBA/2. C57BL/6J generations N6 to N8. More than 10 generations (>N10). The transgenic male mice are crossed to C57BL/6 female mice. The litter size is 10-12 on average.

Phenotype

Neuropathological Analysis:

Tg mice developed Aβ-immunoreactive deposits within the skeletal muscle fibers after 6 to 12 months of age. The muscle fibers with Aβ-immunoreactive deposits increased and became vacuolated with age.

Behavioral:

Mice exhibit hypoactivity and spatial learning deficit associated with higher cytochrome oxidase activity seen in thalamic nuclei.

Availability

Contact:
Dr. Ken-ichiro Fukuchi
Department of Comparative Medicine
Tg13592 Schools of Medicine and Dentistry, University of Alabama at Birmingham,
Kaul 640B, 720 20th Street South,
Birmingham, AL 35294-0019.
Phone: 205-975-8715
Fax: 205-975-5519
Email: fukuchi@uab.edu

Patents: N/A

References

Primary:

Fukuchi K, Ho L, Younkin SG, Kunkel DD, Ogburn CE, Wegiel J, Wisniewski HM, LeBoeuf RC, Furlong CE, Deeb SS, Nochlin D, Sumi SM, Martin GM: High levels of β-amyloid protein in peripheral blood do not cause cerebral β-amyloidosis in transgenic mice. Am J Pathol 149:219-227, 1996. Abstract

Associated:

Fukuchi K., Pham D., Hart M., Li L., Lindsey J.R. ß-Amyloid protein deposition in skeletal muscle of transgenic mice: Possible modes of inclusion body myopathy. Am. J. Pathol. 153, 1687 1693, 1998. Abstract

Fukuchi K, Li L, Hart M, Lindsey JR. Accumulation of amyloid-beta protein in exocrine glands of transgenic mice overexpressing a carboxyl terminal portion of amyloid protein precursor. Int J Exp Pathol. 81(4):231-9, 2000. Abstract

Lalonde R, Dumont M, Fukuchi K, Strazielle C. Transgenic mice expressing the human C99 terminal fragment of betaAPP: effects on spatial learning, exploration, anxiety, and motor coordination. Exp Gerontol., 37(12):1401-12, 2002. Abstract.

Strazielle C, Dumont M, Fukuchi K, Lalonde R. Transgenic mice expressing the human C99 terminal fragment of betaAPP: effects on cytochrome oxidase activity in skeletal muscle and brain. J Chem Neuroanat. 27(4): 237-46, 2004. Abstract.