General Information

Transgene: CMVAPP695sw construct, containing APPsw695, (gift of Tae-Wan Kim). The pNSE-CAT (gift from Dr. J. Gregor Sutcliffe). NSE-APPsw with the prokaryotic sequence eliminated, microinjected into BDF1.

Promoter: Rat NSE

Origin: C57BL/6 x DBA/2

Phenotype

Neuropathological analysis:

12-month-old tg mouse brains showed increased immunostaining for Aß-42 without plaque formations in cortex or hippocampus. In MAPK family, both JNK and p38 were activated, whereas there was no significant activation of the ERK. Tau phosphorylation was also enhanced in the tg relative to the control mice. Cox-2 levels, (WB and immunostaining) and significant caspase-3- and TUNEL-stained nuclei were detected in tg mice compared to the age-matched controls.

Behavioral:

In the water maze tests, 12-month-old transgenic mice had longer escape latencies, across the former platform location, than the age-matched control mice. These transgenic mice also showed increased escape distances in finding the former platform location.

Availability

Yong K. Kim
Division of Laboratory Animal Resources
Korea FDA, National Institute of Toxicological Research
5 Nokbun-dong Eunpyng-ku
Seoul 122-704, Korea
Telephone: +82-2-380-1835
Fax: +82-2-380-1833
Email: kimyongkyu@hanmail.net

Patents: APPsw-Transgenic Mice; Jung S. Cho / Yong K. Kim et al.; Filed 11/4/02

Reference

Primary:

Hwang DY, Cho JS, Lee SH, Chae KR, Lim HJ, Min SH, Seo SJ, Song YS, Song CW, Paik SG, Sheen YY, Kim YK. Aberrant expressions of pathogenic phenotype in Alzheimer's diseased transgenic mice carrying NSE-controlled APPsw. Exp Neurol. 2004 Mar ; 186(1):20-32. Abstract.