Posted 14 November 2008

General Information

Transgene: Human APP751 cDNA with the E693Q mutation was inserted into the blunt-ended XhoI site of the vector pTSC 1 containing the murine Thy1.2 minigene. After removal of vector sequences by NotI/PvuI digestion, linear Thy1-APPconstructs were injected into C57BL/6J oocytes.

Mutation: E693Q (Dutch)

Promoter: Mouse Thy1.2

Strain: C57BL/6J-Tg(Thy1-APPDutch); original background strain: C57BL/6J

Phenotype

Neuropathological Analysis:

APP Dutch mice develop cerebral amyloid angiopathy (in the absence of parenchymal amyloid) at approx. 24 mo of age, Aβ40 >> Aβ42. The line with the highest transgene expression is approx. 5x greater than endogenous APP levels.

Behavioral Phenotype:

N/A

Availability

Contact Mathias Jucker, mathias.jucker@uni-tuebingen.de.

References

Primary:

Herzig MC, Winkler DT, Burgermeister P, Pfeifer M, Kohler E, Schmidt SD, Danner S, Abramowski D, Stürchler-Pierrat C, Bürki K, van Duinen SG, Maat-Schieman MLC, Staufenbiel M, Mathews PM, Jucker M. Abeta is targeted to the vasculature in a mouse model of hereditary cerebral hemorrhage with amyloidosis. Nature Neuroscience 2004; 7(9):954-960. Abstract