Transgene: The 6.0 kbps promoter fragment was subcloned into the
Bam HI/XbaI site of pCMV. A 1.6 kbp fragment containing the human b-globin splice
fragment was subcloned. A cDNA for ApoE3 was reverse transcribed and converted
to ApoE4 by site-directed mutagenesis.
Mutation: ApoE4 cDNA was mutated at residue 28 from Leu to Pro by site
Promoter: rat GFAP gene promoter. , The 990 bp ApoE4 fragment was subcloned
downstream from GFAP promoter and upstream from the b-globin site.
Mouse strain: cDNA were microinjected into the male pronuclei
of B6D2FI zygote. Zygotes were reimplanted into pseudopregnant B6CBAFI females.
Crossed twice with wt C57BL/6 mice.
Homozygous ApoE4(C112R) contained total ApoE proteins 3.5 fold the endogenous ApoE
Homozygous ApoE4(L28P; C112R) contained total ApoE proteins 2 fold the endogenous
ApoE in wt.
Quantitative Western for synaptic proteins, cytoskeletal protein tau, PkC, and APP
show no significant change from wt.
In either passive avoidance task and an active avoidance task, neither Tg mice were
different from the wt.
Licensing/academic distribution contact information:
Gerda Huber email@example.com
Huber G, Marz W, Martin JR, Malherbe P, Richards JG, Sueoka N, Ohm T, Hoffmann MM.
Characterization of transgenic mice expressing apolipoprotein E4(C112R) and apolipoprotein
E4(L28P; C112R). Neuroscience 2000;101(1):211-8.