Transgene: Used a three-way PCR strategy to generated hApoE4 cDNA from hApoE3 cDNA
template. PCR product was gel purified and ligated into TA cloning vector. Clones
containing full length hApoE4 cDNA were then cloned into Xho1 site of MoPrP.Xho
and resulting plasmids gel purified for microinjection into embryos.
Mutation: Human ApoE4
Mouse Strain: C57BL/6J x C3H/HeJ
Tg mice express high levels of hApoE4 in both astroglia and neurons, but only astrocytes
efficiently secreted Apo E4. CNS astrocytes secrete hApoE4 at 5x the normal levels
of murine ApoE. These high levels of expression were not associated with obvious
Not assessed. No obvious behavioral features or change in lifespan.
Univ. North Carolina MMRRC 000399-UNC-RESUS
Phone: (919) 966-9443
Fax: (919) 843-4816
Web site: http://www.mmrrc.org/
Lesuisse C, Guilian Xu G, Anderson J, Wong M, Jankowsky J, Holtz G, Gonzalez V,
Wong PCY, Price DL, Tang F, Wagner S and Borchelt DR. Hyper-expression of human
apolipoprotein E4 in astroglia and neurons does not enhance amyloid deposition in
transgenic mice. Hum Mol Genetics 10: (22) 2525-2537, 2001.