General Information

Transgene: Used a three-way PCR strategy to generated hApoE4 cDNA from hApoE3 cDNA template. PCR product was gel purified and ligated into TA cloning vector. Clones containing full length hApoE4 cDNA were then cloned into Xho1 site of MoPrP.Xho and resulting plasmids gel purified for microinjection into embryos.

Mutation: Human ApoE4

Promoter: MoPrP.Xho1

Mouse Strain: C57BL/6J x C3H/HeJ

Phenotype

Neuropathological Analysis:

Tg mice express high levels of hApoE4 in both astroglia and neurons, but only astrocytes efficiently secreted Apo E4. CNS astrocytes secrete hApoE4 at 5x the normal levels of murine ApoE. These high levels of expression were not associated with obvious neuropathological abnormalities.

Behavioral:

Not assessed. No obvious behavioral features or change in lifespan.

Availability

MMRRC
Cryopreserved
Univ. North Carolina MMRRC 000399-UNC-RESUS
Phone: (919) 966-9443
Fax: (919) 843-4816
Web site: http://www.mmrrc.org/
Email: MMRRCtech@listserv.med.unc.edu

Patents:

References

Primary:

Lesuisse C, Guilian Xu G, Anderson J, Wong M, Jankowsky J, Holtz G, Gonzalez V, Wong PCY, Price DL, Tang F, Wagner S and Borchelt DR. Hyper-expression of human apolipoprotein E4 in astroglia and neurons does not enhance amyloid deposition in transgenic mice. Hum Mol Genetics 10: (22) 2525-2537, 2001. Abstract