| Reagent Recipe: |
10x DNA gel loading buffer |
| |
| Submitted By: | June Kinoshita |
| |
Brief Description:
10x DNA gel loading buffer
Recipe:
| 10x DNA gel loading buffer |
10mL |
4mL |
| 30% glycerol |
3 mL (100% stock) |
1.2 mL |
| 0.1 M EDTA |
4 mL (250 mM stock) |
1.6 mL |
| 1% SDS |
0.5 mL (20% stock) |
0.2mL |
| 0.25% bromophenol blue(BPB) |
0.025g |
0.01 g |
|
H20
|
up to 10 mL |
up to 4 mL |
Migration of BPB relative to DNA is 100-500 bp depending on the percentage of agarose.