Brief Description:
Transfer Buffer
Recipe:
Transfer Buffer
10x Tris Gly Transfer Buffer:
288.4g Glycine (1.92 M)
60.5g Trizma base (0.25M)
2L H20
1x Tris Gly Transfer Buffer:
100mL 10x Tris Gly Stock (final 192mM Glycine, 25mM Trizma base)
200mL MeOH
700mL H20
-Ensure pH = 8-8.5 before use
-Re-use transfer buffer once.