Updated 9 June 2010
Protocol Procedure: View Protocol
We describe methods and detailed protocols for reproducibly preparing Aβ aggregates of defined size distribution and morphology, including monomers, protofibrils and fibrils, using size exclusion chromatography. In addition, we describe detailed biophysical procedures for elucidating the structural features, aggregation kinetics and toxic properties of the different Aβ aggregation states, with special emphasis on protofibrillar intermediates.
Jan A, Hartley DM, Lashuel HA. Characterization of toxic Aβ aggregates for structural and functional studies in Alzheimer's disease research. Nature Protocols. 2010;5:1186-1209.