Updated 19 June 2009
| Protocol: | Acid Phosphatase Histochemistry for Mouse Brain |
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| Submitted By: | Ralph Nixon |
| Lab: |
Nixon |
| PI Name: |
Ralph A. Nixon |
Brief Description:
Autophagic-lysosomal compartments contain numerous acid hydrolases. Analysis using enzyme histochemistry for acid phosphatase at the ultrastructural level can provide information about the trafficking and distribution of acid hydrolases in neuronal processes and perikarya and within specific subcellular compartments. We usually use a lead capture technique with cytidine 5’-monophosphate (CMP) as the substrate.
Protocol Procedure: View Protocol
References:
Cataldo AM, Paskevich PA, Kominami E, Nixon RA. Lysosomal hydrolases of different classes are abnormally distributed in brains of patients with Alzheimer disease. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):10998-1002.
Abstract
Yang DS, Lee JH, Nixon RA. Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. Abstract