Updated 19 June 2009
| Protocol: | Horseradish Peroxidase (HRP) Uptake/Trafficking |
| |
| Submitted By: | Ralph Nixon |
| Lab: |
Nixon |
| PI Name: |
Ralph Nixon |
Brief Description:
Uptake of HRP into neuronal cells occurs via bulk endocytosis, especially at axonal terminals. Endocytosed HRP is transported in a retrograde direction and delivered into autophagic-lysosomal vacuoles in the cell body and, therefore, can serve as a marker for studying endocytic trafficking to autophagic-lysosomal compartments.
Protocol Procedure: View Protocol
References:
Broadwell RD, Brightman MW. Horseradish peroxidase: a tool for study of the neuroendocrine cell and other peptide-secreting cells. Methods Enzymol. 1983;103:187-218. Abstract
Broadwell RD, Oliver C, Brightman MW. Neuronal transport of acid hydrolases and peroxidase within the lysosomal system or organelles: involvement of agranular reticulum-like cisterns. J Comp Neurol. 1980 Apr 1;190(3):519-32. Abstract
Yang DS, Lee JH, Nixon RA. Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. Abstract