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Updated 24 February 2009

Protocol:Extraction of Abeta From Brain Tissues for Application to Abeta ELISA
 
Submitted By:Nobuhisa Iwata
Lab: Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute
PI Name: Takaomi C. Saido

Brief Description:
This protocol is a method to extract Abeta from brain tissues for application to Abeta ELISA.

Protocol Procedure: View Protocol

Categories
Proteins Methods Species
Abeta
Protein Isolation/Purification
All

Equipment:
Teflon-glass homogenizer (Wheaton 1ml tapered tissue grinder), Beckman Optima TL Ultracentrifuge, TLA100.4 rotor, Motor-driven plastic pestle, Evaporator

References:
Saido TC, Iwata N. (2006) Metabolism of amyloid beta peptide and pathogenesis of Alzheimer's disease: Towards presymptomatic diagnosis, prevention and therapy. Neurosci. Res. 54(4): 235-253. Abstract

Huang S-M, Mouri A, Kokubo H, Nakajima R, Suemoto T, Higuchi M, Staufenbiel M, Noda Y, Yamaguchi H, Nabeshima T, Saido TC, Iwata N. (2006). Neprilysin-sensitive synapse-associated Abeta oligomers impair neuronal plasticity and cognitive function. J. Biol. Chem. 281(26): 17941-17951. Abstract

Saito T, Iwata N, Tsubuki S, Takaki Y, Takano J, Huang S-M, Suemoto T, Higuchi M, Saido TC. (2005). Somatostatin regulates brain amyloid beta peptide, Abeta42, via modulation of proteolytic degradation. Nature Med. 11(4): 434-439. Abstract

Iwata N, Mizukami H, Shirotani K, Takaki Y, Muramatsu S, Lu B, Gerard NP, Gerard C, Ozawa K, Saido TC. (2004). Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-beta peptide in mouse brain. J. Neurosci. 24(4): 991-998. Abstract

Iwata N, Tsubuki S, Takaki Y, Shirotani K, Bao L, Gerard NP, Gerard C, Hama E, Lee H-J, Saido TC. (2001). Metabolic Regulation of Brain Abeta by Neprilysin. Science 292(5521): 1550-1552. Abstract

 
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