Updated 23 February 2009

IHC (immunoperoxidase) protocol.

Fisherbrand-Superfrost/Plus slides #12-550-15
Vector- Avidin/Biotin Blocking kit #SP-2001
Vector- elite ABC Kit (standard) #PK6100
Vector secondary antibodies
Biogenex- Liquid DAB Substrate Pack #HK130-5K
Richard Allan Scientific-Cytoseal XYL #8312-4
Triton X-100
Methanol
normal animal serum (species depends on what the 2 degrees antibody is made in)
humid chamber for slides
staining dishes
counterstain (methylene blue, hematoxylin, etc.)
BSA (Sigma A-8022 is fine)
PBS
Antigen Retrieval supplies, if needed