| Protocol: |
Acid Phosphatase Histochemistry for Mouse Brain |
| Method: |
TBD |
| Description: |
Autophagic-lysosomal compartments contain numerous acid hydrolases. Analysis using enzyme histochemistry for acid phosphatase at the ultrastructural level can provide information about the trafficking and distribution of acid hydrolases in neuronal processes and perikarya and within specific subcellular compartments. We usually use a lead capture technique with cytidine 5’-monophosphate (CMP) as the substrate. |
| Lab: |
Nixon |
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| Protocol: |
Horseradish Peroxidase (HRP) Uptake/Trafficking |
| Method: |
TBD |
| Description: |
Uptake of HRP into neuronal cells occurs via bulk endocytosis, especially at axonal terminals. Endocytosed HRP is transported in a retrograde direction and delivered into autophagic-lysosomal vacuoles in the cell body and, therefore, can serve as a marker for studying endocytic trafficking to autophagic-lysosomal compartments. |
| Lab: |
Nixon |
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| Protocol: |
Immunocytochemistry and Immunofluorescence for Brain Sections |
| Method: |
Immunohistochemistry , TBD |
| Description: |
Frequently used on human and mouse brain sections for detecting pathologies in the endocytic-autophagic-lysosomal pathways such as changes in the numbers, types and distribution of vacuoles, and expression levels of individual molecules in the pathways. |
| Lab: |
Nixon |
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| Protocol: |
In vivo Cathepsin D and Cathepsin B Activity Assay |
| Method: |
TBD |
| Description: |
In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in live cell condition. |
| Lab: |
Nixon |
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