Antibodies to Ubiquitin Proteins From The Manufacturers Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin (1B4-UB) IgG1 Abcam Cat# AB122 immunogen = ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin Monoclonal Mouse (liquid, IgG fraction, PBS, azide) ELISA, FC/FACS, IH(F,P), WB ubiquitin Human, cow; others not tested Ubiquitin (10C2-2) IgG1,κ Abcam Cat# AB411   Monoclonal Mouse (supernate, no preservative, lyophilized) AP, ELISA, IH(F,P), WB reacts specifically with 8.5 kDa protein and ubiquitinated proteins   Ubiquitin (-poly) IgG Abcam Cat# AB837 immunogen = ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins Polyclonal rabbit (liquid, protein-A purified) IH(F,P) ubiquitin; also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer disease Human, rat, mouse, cow; others not tested Ubiquitin (-poly) IgG Abcam Cat# AB6181 immunogen = Ubiquitin from Sigma (Product number U6253) that was coupled to chicken gammaglobulin Polyclonal sheep (liquid, whole antiserum) DB, IH(F), WB both free ubiquitin and ubiquitin protein conjugates; labels tangles and Lewy bodies Human, rabbit, sheep; others not tested Ubiquitin (-poly) IgG Abcam Cat# AB6309 immunogen = bovine red blood cell ubiquitin conjugated to KLH Polyclonal rabbit (liquid, whole antiserum, PBS, lactose, no preservative) IF, IH(F), WB ubiquitin Cow, s.cerevisiae; others not tested, expect wide range Ubiquitin (-poly) IgG Abcam Cat# AB6416 immunogen = full length native protein, purified Polyclonal rabbit (liquid, whole antiserum, azide) ELISA, IH(P), WB recognises the 76 amino acid protein ubiquitin which is highly conserved between animal species Human; others not tested Ubiquitin (Ubi-1) IgG1,κ Abcam Cat# AB7254 immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker Monoclonal Mouse (liquid, ascites, azide) IH(F,P), IP, WB recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin; specifically recognizes ubiquitinated cytoplasmic inclusion bodies Chicken, cow, drosophila melanogaster, human, mouse, arabidopsis thaliana, c.elegans; others not tested Ubiquitin (-poly) IgG Abcam Cat# AB7780 immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R Polyclonal rabbit (liquid, whole antiserum, tris, carriers, azide) ELISA, IH(F,P), IP, WB reacts with physiologically occurring conjugates of ubiquitin and intracellular proteins; specifically recognizes ubiquitinated cytoplasmic inclusion bodies Human, rat, horse, mouse, cow; others not tested Ubiquitin (-poly) IgG Abcam Cat# AB8134 supplied by Novus Biologicals immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker Polyclonal rabbit (liquid, whole antiserum) ELISA, IF, IH(F,P), IP, WB recognizes ubiquitinated inclusion bodies Human, chicken, cow, drosophila melanogaster, c.elegans; others not tested Ubiquitin (-poly) IgG Abcam Cat# AB8546 immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R Polyclonal rabbit (liquid, whole antiserum, green food dye, azide, predilute) IH(P) ubiquitin in periphery of senile plaques and neurofibrillary tangles in Alzheimer's disease brain, Lewy bodies in Parkinson's disease and mallory bodies in alcoholic liver disease in humans Human, rat, horse, mouse, cow; others not tested Ubiquitin (P4D1) IgG1k CRP Inc. cat# MMS258R   Monoclonal Mouse WB, ELISA ubiquitin and ubiquitin-substrate conjugates by immuno- blotting. extensive species cross-reativity from yeast to human Ubiquitin (-poly) Biogenesis   Polyclonal rabbit IH   Human, cow, mouse and rat Ubiquitin (-poly) Biogenesis   Polyclonal rabbit Radial Immuno- difusion, WB     Ubiquitin (-poly) Biogenesis   Polyclonal sheep IH, WB, ELISA Ubiquitin Extensive species Ubiquitin (242.9) IgG1 Biogenesis 76 amino acid Monoclonal mouse IH, WB, EM Ubiquitin human Ubiquitin (-poly) Biomeda   Polyclonal rabbit IH     Ubiquitin (-poly) Biomeda   Polyclonal rabbit WB, ELISA, IH 8.5kDa ubiquitin; periphery of senile plaques and of neurofibrillary tangles in Alzheimer's disease brain, the Lewy bodies in Parkinson's disease brain Bovine, human, equine, mouse and rat. Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin (-poly)   Biotrend   Polyclonal rabbit IH ubiquitin cow, mouse, rat Ubiquitin (-poly)   Biotrend   Polyclonal rabbit IDD, WB, Radial Immuno- difusion ubiquitin   Ubiquitin (-poly) Biotrend   Polyclonal sheep IH, ELISA, WB(?) normal, Parkinson's and Alzheimer's disease brain tissue Various species Ubiquitin (242.9) IgG1 Biotrend  76 Amino Acids Monoclonal mouse WB, IH, EM Alzheimer's disease brain tissue, Lewy bodies   Ubiquitin (-poly) Biotrend ubiquitin isolated from cow euythrocytes Polyclonal Rabbit (pre-titer) IH periphery of senile plaques and neurofibrillay tangles in Alzheimer's disease Human and other Mammalian species Ubiquitin (-poly) Biotrend ubiquitin isolated from cow euythrocytes Polyclonal Rabbit (serum Ig, purified, azide) IH periphery of senile plaques and neurofibrillay tangles in Alzheimer's disease Human and other Mammalian species Ubiquitin (-poly) IgG Boehringer Mannheim   Polyclonal rabbit Cryosections, IH, IC, WB ubiquitin, paired helical filaments (PHF) of AD, Pick body and Lewy body human Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin (-poly) Chemicon Cat# AB1586   Polyclonal sheep IH, WB free ubiquitin and ubiquitin-protein conjugates, tangles and Lewy bodies Human, rabbit and sheep Ubiquitin- proteosome (-poly) Chemicon Cat# AB1687   Polyclonal rabbit WB, ELISA ubiquitin- proteosome. Reacts with 8-10 bands between 25-35 kDa Human Ubiquitin (-poly)   Chemicon Cat# AB1690  76 Amino Acid Polyclonal rabbit WB, IH neurofibrillary tangles, neurites in senile plaques. No reactivity seen to tau, neurofilament or MAP.   Ubiquitin- C terminal hydrolase (-poly)   Chemicon Cat# AB1761   Polyclonal rabbit WB, IH in several ubiquinated inclusion bodies, suggesting that such structures may be metabolically dynamic regions of the cell Human, rat and mouse Ubiquitin (-mono) IgG1   Chemicon Cat# MAB1510 reference Giasson, 2002   Monoclonal mouse WB, IH, EM, Analytical Biochemistry ubiquitin, paired helical filaments (PHF) of AD, Pick body and Lewy body   Ubiquitin (P4D1) IgG1 Covance Research Products cat# MMS-257P immunogen = denatured bovine ubiquitin Monoclonal Mouse (purified) ELISA, WB recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins Human, extensive cross, includes yeast Ubiquitin (Ubi-1) IgG1,κ Covance Research Products cat# MMS-264R immunogen = purified KLH-conjugated ubiquitin Monoclonal Mouse (ascites, azide) ELISA, IH, WB ubiquitin; insensitive to formalin fixation, also detects ubiquitin inclusion bodies such as neurofibrillary tangles, Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease Human, bovine, chicken, Drosophila and C. elegans Ubiquitin (P4G7) IgG1 Covance Research Products cat# MMS-258R immunogen = denatured bovine ubiquitin Monoclonal Mouse (ascites) WB recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins Human, extensive cross, includes yeast Ubiquitin (-poly) IgG1 Covance Research Products cat# PRB-268C immunogen = glutaraldehyde cross linked ubiquitin Polyclonal rabbit (serum) IF, WB recognizes ubiquitin Human Ubiquitin (-poly) Dako cat# Z0458 reference Sherer, 2002 reference LaVaute, 2001 immunogen = Ubiquitin isolated from cow erythrocytes and conj. to chicken gamma globulins with glutaraldehyde Polyclonal rabbit (purified Ig fract, azide) CIE, ELISA IB, IC periphery of senile plaques and of neurofibrillary tangles, Lewy bodies, Mallory bodies Human, cow, mouse, rat, others Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin (Ubi-1) IgG1k EnCor Biotech cat# MCA-Ubi1 purified ubiquitin conj. with glutaraldehyde to keyhole limpet hemocyanin Monoclonal Mouse (ascites, purified, BSA, KLH) IF, IH, WB Neurofibrillary tangles strongly and specifically; also recognizes ubiquitinated inclusions and other ubiquitinated inclusion bodies such as Lewy bodies and Pick bodies Human, bovine, chicken, Drosophila, and C. elegans Ubiquitin (-poly) Ig EnCor Biotech cat# RPCA-Ubi glutaraldehyde cross linked ubiquitin in rabbit Polyclonal rabbit (crude serum) IF, WB for detection of Ubiquitinated inclusion bodies such as neurofibrillary tangles of Alzheimer's disease, Lewy bodies, Pick bodies   Ubiquitin (-poly) Fitzgerald   Polyclonal rabbit WB, IH Lewy bodies in Parkinson disease.   Ubiquitin (KM691) IgM Kamiya   Monoclonal mouse WB, IH ubiquitin human Ubiquitin (1B3) IgG1 MBL Cat# MK11-3 reference Imai, 2001 immunogen = purified bovine erythrocyte ubiquitin Monoclonal Mouse (affinity purified, lyophilized, PBS, no preserves) IH, WB reacts with erythrocyte ubiquitin Human, bovine, mouse Ubiquitin (2C5) IgG1 MBL Cat# MK12-3 immunogen = purified bovine erythrocyte ubiquitin Monoclonal Mouse (affinity purified, lyophilized, PBS, no preserves) WB reacts with erythrocyte ubiquitin Human, bovine, mouse Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin+1 (-poly) IgG [for Net-98] R&D Systems Cat # AF703 immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1 Polyclonal rabbit (affinity purified, filtered, PBS, trehalose, lyophilized) IH, WB rhUbiquitin+1 Human Ubiquitin+1 (83406) IgG2B [for Net-98] R&D Systems Cat # MAB701 immunogen = recombinant Ubiquitin+1 containing a His10 tag at the amino terminal Monoclonal Mouse (IgG fract, protein-G, PBS, filtered, lyophilized) IH, WB Ubiquitin and Ubiquitin+1 Human Ubiquitin+1 (82001.11) IgG2A [for Net-98] R&D Systems Cat # MAB703 immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1 Monoclonal Mouse (IgG fract, protein-G, PBS, filtered, lyophilized) WB Ubiquitin+1; not Ubiquitin Human Ubiquitin (1B3) IgG1 RDI   Monoclonal mouse (ascites, affinity purified, azide) FC, WB human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda by SDS-PAGE human, bovine Ubiquitin (2C5) IgG1 RDI   Monoclonal mouse (ascites, affinity purified, azide) FC, IH, WB human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda by SDS-PAGE human, bovine Ubiquitin (-poly) IgG RDI aa 2-20 of the carboxy terminus of ubiquitin of human origin Polyclonal goat IH, WB ubiquitin, neurofibrillary tangles in Alzheimer's disease, Lewy bodies mouse, rat and human Ubiquitin (P1A6) IgG1 RDI aa 1-46 of the amino terminus of ubiquitin of bovine origin Monoclonal mouse FC, IH, WB ubiquitin, poly-ubiquitin and ubiquitinated proteins Multiple species Ubiquitin (FPM1) IgG1 RDI   Monoclonal mosue FC, IH, WB brain sections from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity   Ubiquitin (-poly) RDI   Polyclonal rabbit WB, IH brain sections from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity human, cow, mouse and rat Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity Ubiquitin (-poly) RDI   Polyclonal rabbit WB, IH brain sections from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity   Ubiquitin (P4D1) IgG1 Santa Cruz Cat# sc-8017 reference, Tatebayashi, 2002 epitope corr. to aa 1-76 of full length bovine ubiquitin Monoclonal Mouse (liquid, PBS, azide, gelatin) IH(P), IP, WB reactive with ubiquitin, poly-ubiquitin and ubiquitinated proteins Human, bovine, mouse, rat, others Ubiquitin (-poly) IgG Santa Cruz Cat# sc-9133 reference Imai, 2001 epitope corr. to aa 1-76 of full length human ubiquitin Polyclonal rabbit (liquid, PBS, azide, gelatin) IH(P), IP, WB reactive with ubiquitin, poly-ubiquitin and ubiquitinated proteins Human, bovine, mouse, rat, others Ubiquitin (-poly) Sigma Cat# U5379 reference Caldwell, 2003   Polyclonal rabbit (serum, PBS, predilute, lyophilized) WB, IF     Ubiquitin fluorescein conjugate (-poly)   Sigma   Polyclonal rabbit IF ubiquitin   Ubiquitin (5-25) IgG1k Signet   Monoclonal Mouse (ascites) ELISA, IH, IP, WB residues 64-76 of ubiquitin and ubiquitin in PHF. Formalin fixation eliminates reactivity.   Ubiquitin (3-39) IgG1k Signet   Monoclonal Mouse (ascites) ELISA, IH, IP, WB residues 50-65 of ubiquitin in PHF, not free ubiquitin. Highly reactive on formalin fixed tissues.   Ubiquitin (-poly) Stressgen Cat# SPA-200 reference (rabbit poly) Yoo, 2003 immunogen = ubiquitin purified from bovine red blood cells, conj. to KLH Polyclonal rabbit (whole serum) IH, IP, WB free ubiquitin and ubiquinated proteins; will immuno- precipitate BSA-conjugated ubiquitin human, monkey, mouse, rat, hamster, rabbit, guinea pig, bovine, dog, sheep, chicken, frog, yeast, E. coli, Drosophila, fish Ubiquitin (1B3) IgG1 Stressgen Cat# SPA-201 immunogen = purified bovine erythrocyte ubiquitin Monoclonal Mouse (protein-A, PBS, no preserves, lyophilized) IH, WB ~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-202 Human, bovine Ubiquitin (2C5) IgG1 Stressgen Cat# SPA-202 immunogen = purified bovine erythrocyte ubiquitin Monoclonal Mouse (protein-A, PBS, no preserves, lyophilized) IH, WB ~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-201 Human, bovine Ubiquitin (p4G7-H11) IgG Stressgen Cat# SPA-203 immunogen = natural source bovine ubiquitin Monoclonal Mouse (Ig, protein-G, PBS, PMSF) EIA, WB both mono and poly-ubiquinated proteins Human, mouse, rat, rabbit, bovine, hamster, guinea pig, canine, sheep, chicken, Drosophila, yeast Ubiquitin (FK2) IgG1 Stressgen Cat# SPA-205 immunogen = crude poly ubiquitin lysozyme Monoclonal Mouse (protein-A, Ig, PBS, glycerol) IH, WB ubiquitinated proteins; not free ubiquitin   Ubiquitin (UBI-1) IgG1k Zymed Cat# 13-1600 reference (mono ubiquitn) Finley, 2003 immunogen = purified bovine ubiquitin conj. to carrier protein Monoclonal mouse (liquid, PBS, azide) ELISA, IF, IH, IP, WB ubiquitin, both conjugated and unconjugated; reacts with single chain 8.5 kDa protein Human, bovine, chicken, mouse, Drosophilia, C.Elegans Ubiquitin (UBI-1) IgG1k Zymed Cat# 08-0147 reference Holbert, 2003   Monoclonal mouse (liquid, r.t.u., PBS, BSA, azide) ELISA, IC, IH, WB ubiquitin, both conjugated and unconjugated; reacts with single chain 8.5 kDa protein Human Antigen (Clone) IgG Distributor (Producer) Epitope (Immunogen) Host (Formulation) Methods Specificity Reactivity