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Return to Top
Home: Research: Compendia: Antibodies
 Antibody Directory
Initial Search
Antigen Class:   
(plus) Keywords:   any keywords all keywords
 Search Results: Antigen Class=Bad
Narrow Results
Host: Method: Species Reactivity: Distributor:
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1 to 100 of 162 results
Important Notice: We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.
Antibodies to Bad related Proteins
From the Manufacturers
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(mono)
IgG2a, λ
Abcam
Cat# ab54815
immunogen = recombinant fragment corresp. to aa69-168 of human Bad (AAH01901)   monoclonal mouse
(liquid, PBS, protein A purified, no preservative) 
ELISA, WB  Bad recombinant frag.  human, rat, mouse, cat 
Bad
(1.T.19)
IgG1
Abcam
Cat# ab62480
immunogen = full-length mouse Bad containing a N-terminal His6-tag   monoclonal mouse
(liquid, Tris-glycine saline, azide, glycerol, protein G purified) 
IP, WB  recognizes human Bad, Mr 23kD and mouse Bad, Mr 31kD  human, mouse 
Bad
(64103)
IgG2a
Abcam
Cat# ab10520
immunogen = recomb. N-terminal fragment, mouse  monoclonal rat
(liquid protein-G purified PBS no preservative) 
DB  Bad  mouse
(others not tested, not human;) 
Bad
(Y208)
IgG
Abcam
Cat# ab32445
immunogen = synthetic peptide corresponding to residues in the N-term of human Bad  monoclonal rabbit
(liquid, BSA, glycerol, tris-glycine saline, protein-A purified,) 
DB, FC, IC, IF, IH(P), IP, WB  Bad; no cross-reaction with other Bcl-2 members  human, rat, mouse
(others not tested) 
Bad
(poly)
IgG
Abcam
Cat# ab90435
immunogen = synthetic peptide derived from the sequence of mouse Bad, conjugated to KLH  polyclonal rabbit
(liquid, PBS, BSA, glycerol, azide, protein A purified) 
IH(P), WB; not IP  specifically binds the Bad protein  monkey, human, rat, mouse, hamster, rabbit, pig, sheep, dog, cow, drosophila, chicken 
Bad
(poly)
IgG
Abcam
Cat# ab7975
immunogen = synthetic peptide, mouse, (N-terminal)  polyclonal rabbit
(liquid IgG fraction affinity purified PBS gelatin azide) 
IH(F), WB  detects Bad  rat, mouse
(others not tested) 
Bad
(poly)
IgG
Abcam
Cat# ab31299
immunogen = synthesized non phospho- peptide derived from BAD around the phospho. site of Ser136 (Human)  polyclonal rabbit
(liquid affinity purified, PBS, glycerol, azide) 
ELISA, IH(P), WB  detects endogenous levels of BAD protein around Serine 136  human, rat, mouse 
Bad
(poly)
IgG
Abcam
Cat# ab10806
immunogen = human Bad fused to proprietary tag  polyclonal rabbit
(liquid whole antiserum azide) 
IH(P), WB  detects Bad  human
(others not tested) 
Bad
(poly)
IgG
Abcam
Cat# ab28840
immunogen = synthetic peptide derived from human BAD around the phosphorylation site of Serine 112  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
ELISA, IH(P), WB  detects Bad  human, mouse
(others not tested) 
Bad
(poly)
IgG
Abcam
Cat# ab31310
immunogen = synthetic non-phosphopeptide derived from human Bad around the phosphorylation site of Serine 155  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
ELISA, IH(P), WB  detects endogenous levels of Bad protein around Serine 155.  human, rat, mouse
(others not tested) 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgY
Abcam
Cat# ab13998
immunogen = recombinant fragment, corresp. to aa 2-168 of human Bad  polyclonal chicken
(liquid, PBS, affinity purified) 
WB  Bad  human, rat
(others not tested) 
Bad
(poly)
IgG
Abcam
Cat# ab97733
immunogen = recombinant full length human bad protein (NP_004313  polyclonal rabbit
(liquid, whole antiserum, azide) 
IC, IF, IH(F, P), IP, WB  Bad  human 
Bad
(poly)
IgG
Abcam
Cat# ab62465
immunogen = 15 aa synthetic peptide from near the C- terminal of human Bad   polyclonal rabbit
(liquid, PBS, azide, ion exchange chromatography) 
IH(P), WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Abcam
Cat# ab62647
immunogen = synthesized non- phosphopeptide derived from human Bad around the phosphorylation site of serine 91/ serine 128 (E-L-Sp-P-F).   polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
ELISA, IH(P)  detects endogenous levels of total Bad protein  human, rat, mouse 
Bad
(poly)
IgG
Abcam
Cat# ab62651
immunogen = synthesized non- phosphopeptide derived from human Bad around the phosphorylation site of serine 134 (P-K-SP-A-G)   polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
ELISA, IH(P)  detects endogenous levels of total Bad protein  human, rat, mouse 
Bad
(poly)
IgG
Abcam
Cat# ab59909
immunogen = synthetic peptide selected within aa 100-130 (BH3 domain) of human Bad conjugated to KLH   polyclonal rabbit
(liquid, PBS, azide, protein G purified) 
ELISA, IH(P), IP  recognizes human Bad, Mr 23kD and mouse Bad, Mr 31kD  human 
Bad
(poly)
Accurate
Cat #
YSRT-AHP475 (0.1ml)
Cat #
YSRT-AHP475T (0.02ml)
   polyclonal rabbit
(serum) 
IH(P), WB  Bad   
Bad
(poly)
Accurate
Cat #OBT0491
Discontinued
   polyclonal rabbit
 
  Bad   
Bad
(poly)
Accurate
Cat #
YSGAAP020C and
YSGAAP020E
   polyclonal rabbit
(purified) 
IH, WB  Bad (~23kDa)  monkey, human, rat, mouse, hamster, swine, sheep, goat, bovine, drosophila, chicken 
Bad
(5E6)
Biodesign Meridian LifeScience
cat# K67650M
immunogen = recombinant human Bad  monoclonal mouse
(lyophilized, PBS, PEG, sucrose, azide, purified) 
ELISA, IB, IP  Bad; no cross with Bad/Bcl heterodimers in IP  human 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
Biodesign Meridian LifeScience
cat# K01314R
immunogen = synthetic non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)  polyclonal rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) 
IH, WB  total Bad (aa110-114)  human, rat, mouse 
Bad
(5E6)
IgG1
Biomol Enzo Life Sciences
cat# ALX-804-384
immunogen = recombinant human BAD  monoclonal mouse
(liquid, PBS, PEG, sucrose, azide, purified) 
ELISA, IP, WB  BAD  human 
Bad
(5E6)
IgG1
Biomol Enzo Life Sciences
cat# ADI-905-638-100
immunogen = recombinant human BAD  monoclonal mouse
(lyophilized, PBS, PEG, sucrose, azide, purified) 
IF, IH, IP, WB  BAD  human 
Bad
(poly)
Biomol Enzo Life Sciences
cat# ADI-AAP-020-D (50 µg)
Cat# ADI-AAP-020-F (200 µg)
immunogen = synthetic peptide derived from the sequence of mouse Bad  polyclonal sheep
(liquid, PBS, glycerol, azide, protein A purified) 
IH, WB  BAD  monkey, human, rat, mouse, hamster, rabbit, pig, sheep, dog, bovine, drosophila, chicken 
Bad
(poly)
IgG
Calbiochem EMD Biosciences
cat# 195872
immunogen = synthetic peptide (K S D P G I R S L G S D A G G R R) corresp. to aa 19-35 of mouse Bad, coupled to KLH  polyclonal rabbit
(liquid, borate buffered saline, EDTA, no preservative, affinity purified) 
IB, IH(P), WB  Bad (~22-23kDa); recognizes a 50 kDa band in AK431 human fibroblasts lysates  monkey, human, rat, mouse, hamster, rabbit, porcine, ovine, dog, bovine 
Bad
(11E3)
IgG
Cell Signaling
cat# 9268
immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Ala107 of Bad   monoclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, purified) 
IP, WB  endogenous total Bad  monkey, human, rat, mouse 
Bad
(D24A9)
IgG
Cell Signaling
cat# 9239
immunogen = synthetic peptide corresp. to residues surrounding Pro102 of human Bad   monoclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) 
WB  endogenous total Bad; no cross-reaction with related proteins  monkey, human, rat, mouse 
Bad
(poly)
Cell Signaling
cat# 9292
immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Ser112 of mouse Bad   polyclonal rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, affinity purified) 
IP, WB  endogenous total Bad; no cross with related proteins  monkey, human, rat, mouse 
Bad
(kit)
Cell Signaling
cat# 7162
  (capture ab = anti-bad coated wells; detection ab = Bad)  ELISA  endogenous levels of total Bad  monkey, human 
Bad
(kit)
Cell Signaling
cat# 7840
  (Bad capture ab; Bad detection ab; anti-mouse IgG, HRP-linked ab)  ELISA  total Bad  monkey, human 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Chemicon and Upstate are Millipore
Cat# AB2925
immunogen = peptide of aa 87-103 of human Bad  polyclonal rabbit
(liquid, whole serum, no preservative) 
IB, IH, IP  Bad  human 
Bad
(BV48T)
IgG1
MBL International
cat#
JM-3005-100
Discontinued
immunogen = purified murine Bad protein  monoclonal mouse
(liquid, PBS, BSA, glycerol) 
IH, IP, WB  detect the ~23 kDa Bad protein  human, rat, mouse 
Bad
(poly)
IgG
MBL International
cat# 591
immunogen = full-length aa 1-204 GST-mouse BAD  polyclonal rabbit
(liquid, PBS, glycerol, protein A/affinity purified) 
WB  Bad; 30kDa in mouse and 25kDa in human  human, mouse 
Bad
(poly)
IgG
MBL International
cat# JM-3030-100
Discontinued
immunogen = purified murine Bad protein  polyclonal rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) 
IH, IP, WB  detect the ~22 kDa Bad protein; no cross-reaction with related family members  monkey, human, rat, mouse 
Bad
(3H8)
IgG2a, λ
Novus Biologicals
Cat# H00000572-M02
supplied by Abnova; available in North America only
immunogen = partial recombinant BAD  monoclonal mouse
(liquid, purified, no preservative) 
WB  BAD - BCL2-antagonist of cell death  human 
Bad
(64103)
IgG2a
Novus Biologicals
Cat# NB120-10520
produced by Abcam
immunogen = recomb. N-terminal fragment, mouse  monoclonal rat
(liquid, protein-G purified, PBS, no preservative) 
DB  Bad; also detect a 32 kDa band that comigrates with recombinant Bad on immunoblots  mouse
(not human; others not tested) 
Bad
(Y208)
IgG
Novus Biologicals
Cat# NB200-615
immunogen = synthetic peptide corresp. to residues in the N-term of human Bad  monoclonal rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide) 
FC, IC, IH, IP, WB  Bad; no cross-reaction with other Bcl-2 members  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB120-7975
immunogen = synthetic peptide mapping to the N-terminal domain of mouse Bad  polyclonal rabbit
(liquid, affinity purified, PBS, gelatin, azide) 
IH(F), WB  Bad  rat, mouse
(others not tested) 
Bad
(poly)
IgY
Novus Biologicals
Cat# NB 300-344
Discontinued
immunogen = recombinant fragment, corresp. to aa 2-168 of human Bad  polyclonal chicken
(liquid, PBS) 
IH, WB  Bad  human, rat
(others not tested) 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB200-596
immunogen = full length GST-human BAD fusion protein  polyclonal rabbit
(liquid, azide) 
IH, WB  BAD  human 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-55939
Discontinued
  polyclonal rabbit
(liquid, affinity purified, PBS, gelatin, azide) 
IH(P), WB  Bad  human, rat, mouse
(others not tested) 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-56078
immunogen = synthetic peptide corrsp. to aa87-103 (G E E P S P F R G R S R S A P P N) of human Bad  polyclonal rabbit
(liquid, sera) 
IH(P), IP, WB  Bad  human, rat, mouse
(other species not tested) 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-56079
  polyclonal rabbit
(liquid, sera) 
IH(F, P), IP, WB  Bad  rat, mouse
(other species not tested) 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-56080
immunogen = full length recombinant protein corresponding to human Bad  polyclonal rabbit
(liquid, whole antiserum, PBS, azide) 
IC, IF, IH(F, P), IP, WB  Bad  human
(other species not tested) 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-19451
immunogen = synthetic peptide corresp. to the residues 110-160 of human BAD  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
IF, IH(P), WB  recognizes an epitope within residues 110-160 of human BAD  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-92045
immunogen = synthetic peptide corresp. to the residues surrounding Isoleucine 106 of human BAD  polyclonal rabbit
(liquid, PBS, azide, protein A purified) 
ELISA, IH(P), WB  recognizes an aa near Isolucine 106  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-81806
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E)  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
IH, WB  detects endogenous levels of total BAD protein (specific to a.a. 155)  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-81804
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
IH, WB  detects endogenous levels of total BAD protein (specific to a.a. 112)  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-81805
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 136 (S-R-Sp-A-P)  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
IH, WB  detects endogenous levels of total BAD protein (specific to a.a. 136)  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-19353
immunogen = synthetic peptide corresp. to the residues 120-168 of human BAD  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
IH(P), WB  recognizes an epitope within residues 120-168 of human BAD  human, rat, mouse 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-91672
immunogen = synthetic peptide corresp. to the residues surrounding Arginine 149 of human BAD  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
ELISA, IH(P), WB  recognizes an amino acid near Arginine149  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-41686
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine136 (S-R-Sp-A-P)  polyclonal rabbit
(liquid, PBS, azide, glycerol, affinity purified) 
IH, WB  recognizes an epitope around ser136 of BAD  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-41685
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-Sp-Y-P)  polyclonal rabbit
(liquid, PBS, azide, glycerol, affinity purified) 
IH, WB  recognizes an epitope around ser112 of BAD  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NB100-94296
immunogen = peptide corresp. to 15aa near the C-terminus of human Bad (GenBank accession no. Q92934)  polyclonal rabbit
(liquid, PBS, azide) 
IH, WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-45367
immunogen = synthetic peptide surrounding aa115 of mouse Bad  polyclonal rabbit
(liquid, PBS, BSA, thimerosal, affinity purified) 
IH, IP, WB  Bad (22kDa); no cross-reaction with related family members  human 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-20004
immunogen = synthetic peptide corresp. to the residues surrounding Threonine 118 of human BAD  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
IH  Bad aa near Thr118  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-41687
immunogen = synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E)  polyclonal rabbit
(liquid, PBS, azide, glycerol, affinity purified) 
IH, WB  BAD  human, rat, mouse 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-04928
immunogen = BAD fusion protein (2-168 aa)  polyclonal chicken
(lyophilized, no preservative, affinity purified) 
WB  BCL2-binding protein (BAD)  human, rat 
Bad
(poly)
IgG
Novus Biologicals
Cat# NBP1-35323
immunogen = full length recombinant human BAD protein  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
WB  Bad  human 
Bad
(poly)
Novus Biologicals
Cat# H00000572-D01
supplied by Abnova
immunogen = BAD (NP_004313.1, aa1-168) full-length human protein  polyclonal rabbit
(liquid, no preservative, protein A purified) 
ELISA, WB  BAD - BCL2-antagonist of cell death  human 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Novus Biologicals
Cat# 3506002
Manufactured by SDIX's proprietary Genomic Antibody Technology™
  polyclonal rabbit
(liquid, KPBS, no preservative, affinity purified) 
ELISA, WB  Bad N-terminus  human 
Bad
(poly)
Novus Biologicals
Cat# H00000572-B01
supplied by Abnova
immunogen = BAD (NP_004313.1, aa1-168) full-length human protein  polyclonal mouse
(liquid, antiserum, no preservative) 
ELISA, WB  BAD - BCL2-antagonist of cell death  human 
Bad
(48)
IgG2b
BD Pharmingen
Cat# 610391 and 610392
immunogen = peptide mapping from aa 39 to 198 of mouse Bad  monoclonal mouse
(liquid, PBS, BSA, glycerol, azide) 
IF(C), IH(C), WB  Bad (23kDa)  human, rat, mouse 
Bad
(48)
IgG2b
BD Pharmingen
Cat# 610395 and 610396
immunogen = peptide mapping from aa 39 to 198 of mouse Bad  monoclonal mouse
(liquid, PBS, BSA, glycerol, azide, conj. TRITC) 
IF(C)  Bad (23kDa)  human, rat, mouse 
Bad
(48)
IgG2b
BD Pharmingen
Cat# 610393 and 610394
immunogen = peptide mapping from aa 39 to 198 of mouse Bad  monoclonal mouse
(liquid, PBS, BSA, glycerol, azide, conj. FITC) 
IF(C)  Bad (23kDa)  human, rat, mouse 
Bad
(64103)
IgG2a
R&D Systems
Cat #
MAB-850
immunogen = purified N-terminal half of recombinant mouse Bad  monoclonal rat
(supernat, protein-G purified, PBS, azide, lyophilized) 
WB  Bad  mouse
(not human) 
Bad
(poly)
IgG
R&D Systems
Cat #
AF-819
immunogen = KLH coupled synthetic peptide (M F Q I P E F E P S E Q E D S S S A E R G +C) corr. to aa 1-21 of human Bad  polyclonal rabbit
(affinity purified, PBS, trehalose, lyophilized) 
WB  Bad  human, mouse 
Bad
(mono)
IgG1
Santa Cruz
Cat# sc-8044
Cat# sc-8044 AC (agarose)
immunogen = aa 1-168 representing full length human Bad  monoclonal mouse
(liquid, PBS, gelatin, azide) 
IF, IH(P), IP, WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Santa Cruz
Cat# sc-6541
Cat# sc-6541 P (blocking peptide)
immunogen = peptide mapping near the C-terminus of human Bad; differs from mouse by two amino acids)  polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) 
IF, WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Santa Cruz
Cat# sc-6542
Cat# sc-6542 P (blocking peptide)
immunogen = peptide mapping at the N-terminus of human Bad  polyclonal goat
(liquid, PBS, gelatin, azide, affinity purified) 
IF, IP, WB  Bad  human, rat, mouse 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Santa Cruz
Cat# SC-7869
immunogen = aa 1-168, full length human Bad  polyclonal rabbit
(liquid, PBS, gelatin, azide) 
IF, IP, WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Santa Cruz
Cat# sc-942
Cat# sc-942 G (goat)
Cat# sc-942 P (blocking peptide)
immunogen = peptide mapping near the N- terminus of mouse Bad  polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified) 
IF, IP, WB  Bad  human, rat, mouse 
Bad
(poly)
IgG
Santa Cruz
Cat# sc-943
Cat# sc-943 G (goat)
Cat# sc-943 P (blocking peptide)
immunogen = C-terminus of mouse Bad  polyclonal rabbit
(liquid, PBS, gelatin, azide, affinity purified) 
IF, IP, WB  Bad  rat, mouse 
Bad
(poly)
AbD Serotec
Cat #
AHP-475
immunogen = highly purified GST-human Bad fusion protein  polyclonal rabbit
(liquid, serum, azide) 
IH(P), WB  Bad  human 
Bad
(poly)
AbD Serotec
Cat #
AHP-475T
immunogen = highly purified GST-human Bad fusion protein  polyclonal rabbit
(liquid, serum, azide) 
IH(P)  Bad  human 
Bad
(3H8)
IgG2a
Sigma Aldrich
Cat# WH0000572M2
immunogen = BAD (AAH01901, aa69-168) partial recombinant protein with GST tag  monoclonal mouse
(liquid, PBS, purified) 
ELISA, IB  Bad   
Bad
(64103)
IgG2a
Sigma Aldrich
Cat# B0559
immunogen = purified N-terminal portion of recombinant mouse Bad  monoclonal rat
(purified, filtered, PBS, lyophilized) 
IB  Bad  mouse
(not human) 
Bad
(poly)
Sigma Aldrich
Cat# B0684
immunogen = synthetic peptide (M F Q I P E F E P S E Q D S S S A E R G) corr. to aa 1-21 of human Bad  polyclonal rabbit
(affinity purified, PBS, azide, lyophilized) 
IB  Bad  human, mouse 
Bad
(poly)
Sigma Aldrich
Cat# GW22764
immunogen = recombinant BCL2-antagonist of cell death protein  polyclonal chicken
(liquid, PBS, azide, glycerol, affinity purified) 
IB  Bad  human, rat 
Bad
(poly)
IgG
Sigma Aldrich
Cat# SAB3500336
immunogen = peptide corresp. to 15aa near the C-terminus of human Bad  polyclonal rabbit
(liquid, PBS, azide) 
ELISA, IB, IH, WB  Bad  human, rat, mouse 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad
(poly)
IgG
Sigma Aldrich
Cat# HPA028185
immunogen = BCL2-associated agonist of cell death (BAD) recombinant protein epitope signature tag (PrEST)  polyclonal rabbit
(liquid, PBS, azide, glycerol, affinity purified) 
IB, IH(P), WB  Bad  human 
Bad
(5E6)
IgG1
Stressgen Enzo Life Sciences
Cat# ADI-905-638
immunogen = recombinant human Bad  monoclonal mouse
(lyophilized, PBS, azide, PEG, sucrose, thiophilic adsorption and size exclusion chromotagraphy) 
IF, IH, IP  reognizes Bad (23 kDa); not Bad/Bcl-2 hetrodimers in IP assays  human 
Bad
(5E6)
IgG1
Stressgen Enzo Life Sciences
Cat# ALX-804-384
immunogen = recombinant human Bad  monoclonal mouse
(liquid, PBS, azide, PEG, sucrose, thiophilic adsorption and size exclusion chromotagraphy) 
ELISA, IP, WB  reognizes Bad (23 kDa); not Bad/Bcl-2 hetrodimers in IP assays  human 
Bad
(5E6)
IgG1
Stressgen Enzo Life Sciences
Cat# ALX-804-384
immunogen = recombinant human Bad  monoclonal mouse
(lyophilized, PBS, azide, PEG, sucrose, thiophilic adsorption and size exclusion chromotagraphy) 
IC, IH, IP, WB  reognizes Bad (23 kDa); not Bad complexed with Bcl-XL  human 
Bad
(poly)
Stressgen Enzo Life Sciences
Cat# ADI-AAP-020D, 50 µg
ADI-AAP-020F, 200 µg
immunogen = synthetic peptide corresp. to aa. 19-35 of mouse Bad, coupled to KLH  polyclonal rabbit
(liquid, PBS, glycerol, azide, affinity purified) 
IH, WB  recognizes Bad (23 kDa)  monkey, rat, mouse, hamster, rabbit, pig, sheep, dog, bovine, drosophila, chicken 
Bad
(BYC001)
IgG
Upstate and Chemicon are Millipore
Cat# 05-605
immunogen = full-length murine Bad containing a N-terminal His6-tag   monoclonal mouse
(liquid, tris-glycine saline, glycerol, azide, protein G purified) 
IP, WB  Bad (23kDa human and 31kDa mouse)  human, mouse
(others not tested) 
Bad (5E6)
(mono)
IgG1
Santa Cruz
Cat# sc-81442
immunogen = recombinant human Bad  monoclonal mouse
(liquid, PBS, azide, PEG, sucrose) 
IP, WB  Bad  human 
Bad (ab-112)
(poly)
Sigma Aldrich
Cat# SAB4300341
immunogen = peptide seq. around aa. 110-114 (H-S-S-Y-P), according to the protein BAD  polyclonal rabbit
(lyophilized, azide, glycerol, affinity purified) 
IB, IH, WB  Bad  human, rat, mouse 
Bad (ab-136)
(poly)
Sigma Aldrich
Cat# SAB4300342
immunogen = peptide seq. around aa. 134-138 (S-R-S-A-P), according to the protein BAD  polyclonal rabbit
(lyophilized, azide, glycerol, affinity purified) 
IB, IH(P), WB  Bad  human, rat, mouse 
Bad (ab-155)
(poly)
Sigma Aldrich
Cat# SAB4300343
immunogen = peptide seq. around aa. 153-157 (R-M-S-D-E), according to the protein BAD  polyclonal rabbit
(lyophilized, azide, glycerol, affinity purified) 
IB, IH(P), WB  Bad  human, rat, mouse 
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity      Reactivity     top
Bad (cleaved)
(poly)
IgG
Novus Biologicals
Cat# NB100-56602
  polyclonal rabbit
(liquid, affinity purified) 
WB  Bad, cleaved  human
(other species not tested) 
Bad (cleaved)
(poly)
IgG
Novus Biologicals
Cat# NB100-92714
Immunogen: synthetic peptide corresp. to the residues surrounding Aspartate 71 of human cleaved-BAD  polyclonal rabbit
(liquid, PBS, azide, affinity purified) 
ELISA, IH(P), WB  detects endogenous levels of Cleaved-BAD protein  rat, mouse 
Bad (pSer112)
(poly)
IgG
Calbiochem EMD Biosciences
cat# PC636
Discontinued
immunogen = a synthetic phospho-peptide corresp. to aa surrounding Ser112 of human Bad  polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) 
IB  recognizes the ~23 kDa Bad protein only when phosphorylated at Ser112  human, rat, mouse 
Bad (pSer112)
(poly)
IgG
Calbiochem EMD Biosciences
cat# PC518
immunogen = a synthetic phospho-peptide corresp. to aa surrounding Ser112 of mouse Bad, conj. to KLH  polyclonal rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative, affinity purified) 
IB, IP  recognizes the ~23 kDa Bad protein only when phosphorylated at Ser112. no cross-reaction with phospho-Bad Ser136 or any other phosphorylated member of the Bcl-2 family.  human, rat, mouse 
Bad (pSer112)
(poly)
IgG1
MBL International
cat#
BV-3269-3
  polyclonal rabbit
(liquid, PBS, azide, BSA, glycerol, affinity purified) 
IP, WB  detect Bad when phosphorylated at Ser112  human, rat, mouse 
Bad (pSer136)
(poly)
IgG
Calbiochem EMD Biosciences
cat# PC637
Discontinued
immunogen = a synthetic phospho-peptide corresp. to aa surrounding Ser136 phosphorylation site of human Bad  polyclonal rabbit
(liquid, PBS, BSA, azide, affinity purified) 
IB  recognizes the ~23 kDa Bad protein only when phosphorylated at Ser136. No cross-reaction with phospho-Bad Ser112 or any other phosphorylated member of the Bcl-2 family  human, rat, mouse 
Bad (pSer136)
(poly)
IgG
Calbiochem EMD Biosciences
cat# PC519
immunogen = a synthetic phospho-peptide corresp. to aa surrounding Ser136 of mouse Bad, conj. to KLH  polyclonal rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative, affinity purified) 
IB  recognizes the ~22 kDa Bad protein only when phosphorylated at Ser136. no cross-reaction with unphosphorylated Bad or phospho-Bad Ser112 or any other phosphorylated member of the Bcl-2 family.  human, rat, mouse 
Bad (Ser112)
(poly)
Accurate
Cat #
YSGAAP021E
   polyclonal rabbit
(purified) 
WB  Bad (~23kDa) phos Ser112  human, rat, mouse 
Bad (Ser136)
(poly)
Accurate
Cat #
YSGAAP022E
   polyclonal rabbit
(purified) 
WB  Bad (~23kDa) phos Ser136  human, rat, mouse 
Bad antibody duet
(kit)
Cell Signaling
cat# 8223
  (#5284 Bad phos Ser112 rabbit mAb
#9239 Bad rabbit mAb) 
WB    monkey, human, rat, mouse 
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