|
Antibodies to Vesicular Acetylcholine Transporter
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAChT
(poly)
|
Abcam
Cat #ab31544 |
immunogen =
synthetic peptide: C-T R S R S E R D V L L D E P P Q G L Y D A V R L R E, corresp. to aa 475-500 of rat VAChT conj. to blue carrier protein
|
polyclonal sheep
(liquid, whole sera) |
WB |
VAChT |
rat, guinea pig
(expect human, mouse, dog, chimpanzee, macaque monkey; other speceis not tested) |
VAChT
(poly)
|
Abcam
Cat #ab43875 |
immunogen =
synthetic peptide: C S P P G P F D G C E D D Y N Y Y S R S, corresp. to C- terminal aa 511-530 of rat VAChT
|
polyclonal goat
(liquid, whole sera, azide) |
IC, IF, IH(P), WB |
VAChT |
rat
(other speceis not tested) |
VAChT
(poly)
IgG
|
Abcam
Cat #ab43852 |
immunogen =
synthetic 20 aa sequence peptide from the rat VAChTprotein, conj. with thyroglobulin using a glutaraldehyde linker
|
polyclonal goat
(liquid, PBS, BSA, azide) |
IC, IF, IH(P), WB |
VAChT; no reaction with the vesicular monoamine transporter (VMAT) |
rat, mouse
(not rabbit, guinea pig or cat; other speceis not tested) |
VAChT
(poly)
IgG
|
Abcam
Cat #ab27941 |
immunogen =
C S P P G P F D G C E D D Y N Y Y S R S, corresp. to C terminal aa 511-530 of rat VAChT
|
polyclonal goat
(liquid, PBS, no preservative, protein G purified) |
IH(F) |
VAChT; stains fibers and neuronal cell bodies in CNS and PNS |
rat, mouse
(not human, rabbit, guinea pig or cat; other speceis not tested) |
VAChT
(poly)
IgG
|
Accurate
Cat #
ICCB26-1 |
immunogen = synthetic peptide corr. to the C terminus of rat VAChT, conj. to BSA |
polyclonal rabbit
|
IH |
recognizes VAChT; good marker for cholinergic nerves |
rat, mouse |
VAChT
(poly)
IgG
|
Accurate
Cat #
ICCB27-1 |
immunogen = synthetic peptide corr. to the C terminus of human VAChT, conj. to BSA |
polyclonal rabbit
|
IH |
recognizes VAChT; good marker for cholinergic nerves |
human |
VAChT
(poly)
IgG
|
Accurate
Cat# ICCB GP275 |
immunogen = synthetic peptide corr. to the C terminal of rat VAChT, conj. to BSA |
polyclonal guinea pig
|
IH |
recognizes VAChT; good marker for cholinergic nerves |
rat |
VAChT
(poly)
|
ATS
Cat# AB-N11 |
immunogen = synthetic peptide corr. to the C-terminus of rat VAChT conj. to bovine thyroglobulin |
polyclonal goat
(affinity purified, liquid, PBS, no preserve) |
FC, IH |
VAChT; useful in visualizing acetylcholine (ACh)-containing neurons |
rat |
VAChT
(poly)
IgG
|
Autogen Bioclear
Cat# SC15315
produced by Santa Cruz |
immnuogen = recombinant protein corr. to aa 251-410 mapping near C-terminus of human VAChT |
polyclonal rabbit
(liquid, PBS, azide, gelatin) |
IH, IP, WB |
VAChT |
human, rat, mouse |
VAChT
(poly)
IgG
|
Autogen Bioclear
Cat# SC7716
produced by Santa Cruz |
immnuogen = peptide mapping at the C-terminus of human VAChT; differs from mouse seq. by 2 amino acids |
polyclonal goat
(affinity purified, PBS, azide, gelatin) |
IH, WB |
VAChT |
human, rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAChT
(poly)
IgG
|
Autogen Bioclear
Cat# SC7717
produced by Santa Cruz |
immnuogen = peptide mapping at the N-terminus of human VAChT; differs from mouse seq. by single amino acid |
polyclonal goat
(affinity purified, PBS, azide, gelatin) |
IH, IP, WB |
VAChT |
human, rat, mouse |
VAChT
(poly)
|
Biomol Enzo Life Sciences
Cat #
SA-109 |
immunogen = synthetic peptide corr. to the C-terminus of rat VAChT |
polyclonal goat
(neat serum) |
IH |
VAChT |
rat, guinea pig, mouse
(not rabbit, cat or guinea pig) |
VAChT
(poly)
|
Chemicon and Upstate are Millipore
Cat# AB-1578 |
immunogen = synthetic peptide corr. to aa 511-530 from cloned rat VAChT |
polyclonal goat
(antiserum, liquid, no preserve) |
IC, WB |
VAChT; labeling of the interpeduncular nucleus of the brain stem and ventral horn of the spinal cord |
rat, guinea pig, mouse
(not feline, guinea pig or rabbit) |
VAChT
(poly)
Ig
|
Chemicon and Upstate are Millipore
Cat# AB-1588 |
immunogen = synthetic peptide corresponding to the C-terminus of predicted rat VAChT |
polyclonal guinea pig
(purified Ig, PBS, BSA, azide, lyophilized) |
IC, IH, WB |
VAChT; CNS staining was strong in fibers and stains cholinergic cell bodies |
rat |
VAChT
(poly)
IgG
|
CN Biosciences
Cat# 677000 |
immunogen = C-terminus of rat VAChT |
polyclonal goat
(diluted serum, lyophilized) |
IH |
VAChT. The antibody conjugated to saporin is useful for the elimination of cholinergic neurons |
rat, guinea pig, mouse, ferret
(not feline, guinea pig or rabbit) |
VAChT
(poly)
|
Affiniti - Biomol
Cat# SA109 |
immunogen = peptide corr. to the C-terminus of rat VAChT |
polyclonal goat
(neat serum) |
IH |
VAChT |
rat, guinea pig, mouse
(not rabbit, cat or guinea pig) |
VAChT
(poly)
|
Euro-Diagnostica AB
Cat #
B-26-1 |
immunogen = synthetic peptide from the C-terminal of rat conjugated to BSA |
polyclonal rabbit
(lyophilized) |
IF, IH(F, P) |
VAChT |
rat, mouse |
VAChT
(poly)
|
Euro-Diagnostica AB
Cat #
B-27-1 |
immunogen = synthetic peptide from the C-terminal of human conjugated to BSA |
polyclonal rabbit
(lyophilized) |
IF, IH(F) |
VAChT |
human |
VAChT
(poly)
|
Euro-Diagnostica AB
Cat #
B-275-1 |
immunogen = synthetic peptide from the C-terminal of rat conjugated to BSA |
polyclonal guinea pig
(lyophilized) |
IF |
VAChT both in CNS and PNS |
rat, mouse |
VAChT
(poly)
IgG
|
Fisher Scientific
Cat# PR-G4481
produced by Promega, G4481 |
immunogen = synthetic peptide corresponding to amino acids 511-530 from cloned rat VAChT |
polyclonal goat
(liquid, PBS, no preserves) |
IC, IH, WB; not ELISA |
VAChT; fibers and cell bodies in CNS and PNS |
rat, guinea pig, mouse
(not human, feline, guinea pig or rabbit) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAChT
(poly)
IgG
|
Fisher Scientific
Cat# 5053074
produced by chemicon, AB1588 |
immunogen = synthetic peptide corresponding to the C-terminus of predicted rat VAChT |
polyclonal guinea pig
(purified Ig, PBS, BSA, azide, lyophilized) |
IC, IH, WB |
VAChT; CNS staining was strongin fibers and stains cholinergic cell bodies |
rat |
VAChT
(poly)
|
Interchim
Cat# AB1578
produced by Chemicon |
immunogen = synthetic peptide corr. to aa 511-530 from cloned rat VAChT |
polyclonal goat
(antiserum, liquid, no preserve) |
IC, WB |
VAChT; labeling of the interpeduncular nucleus of the brain stem andventral horn of the spinal cord |
rat, guinea pig, mouse
(not feline, guinea pig or rabbit) |
VAChT
(poly)
|
Interchim
Cat# VAT11S
produced by ADI |
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT) |
polyclonal chicken
(neat serum, azide, available affinity purified) |
ELISA, IF, IH, WB |
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber |
rat, mouse
(others not tested) |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18491
available in the USA only |
immunogen =
synthetic peptide from the cloned rat VAChT
|
polyclonal goat
(unpurified serum) |
IF, IH, WB |
VAChT |
rat, guinea pig, mouse, rabbit, cat |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18488 |
immunogen =
synthetic peptide from the C-terminus of rat VAChT conj. to BSA
|
polyclonal rabbit
(unpurified serum) |
IF, IH(F, P) |
VAChT in both CNS and PNS |
rat, mouse
(not human) |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18492
available in the USA only |
immunogen =
synthetic peptide from the C-terminus of rat VAChT |
polyclonal guinea pig
(DEAE-column chromatography) |
IH(F, P), WB |
VAChT |
rat |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18493
available in the USA only |
immunogen =
20 aa peptide within the cytoplasmic, C-terminal domain of the rat/mouse VAT
|
polyclonal chicken
(unpurified serum) |
ELISA, WB |
VAChT |
rat, mouse |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18494
available in the USA only |
immunogen =
20 aa synthetic peptide within the cytoplasmic, C-terminal of the rat/mouse VAT coupled to KLH
|
polyclonal chicken
(DEAE-column chromatography) |
ELISA, WB |
VAChT |
rat, mouse
(human homology 75%) |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18489
available in the USA only |
immunogen =
synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal guinea pig
(unpurified serum) |
IH(F) |
VAChT in both CNS and PNS |
rat, mouse |
VAChT
(poly)
|
LifeSpan Biosciences, Inc.
Cat #LS-C18490 |
immunogen =
synthetic peptide from the C-terminus of human VAChT conj. to BSA
|
polyclonal rabbit
(unpurified serum) |
IH |
VAChT in both CNS and PNS |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAChT
(poly)
IgG
|
BD Pharmingen
Cat #
556337 |
immunogen = synthetic peptide corr. to the C-terminus of rat VAChT |
polyclonal goat
(liquid, antiserum, azide) |
IH(F) |
VAChT; not vesicular monoamine transporter |
rat, guinea pig, mouse
(not rabbit, cat or guinea pig) |
VAChT
(poly)
|
PROGEN Biotechnik GmbH
Cat# 16084 |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal guinea pig
(antiserum, lyophilized) |
IH(F) |
VAChT in both CNS and PNS |
rat, mouse |
VAChT
(poly)
|
PROGEN Biotechnik GmbH
Cat# 16087 |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal rabbit
(antiserum, lyophilized) |
IF, IH(F, P) |
VAChT in both CNS and PNS |
rat, mouse
(not human) |
VAChT
(poly)
|
PROGEN Biotechnik GmbH
Cat# 16095 |
immunogen = synthetic peptide from the C-terminus of human VAChT conj. to BSA |
polyclonal rabbit
(antiserum, lyophilized) |
IH(F) |
VAChT |
human |
VAChT
(poly)
IgG
|
Promega
Cat# G4481 |
immunogen = synthetic peptide corresponding to amino acids 511-530 from cloned rat VAChT |
polyclonal goat
(liquid, PBS, no preserves) |
IC, IH, WB; not ELISA |
VAChT; fibers and cell bodies in CNS and PNS |
rat, guinea pig, mouse
(not human, feline, guinea pig or rabbit) |
VAChT
(poly)
|
RDI /Fitzgerald
Cat# PRO16084 |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal guinea pig
(antiserum, lyophilized) |
IF, IH(F, P) |
VAChT in both CNS and PNS |
rat, mouse |
VAChT
(poly)
|
RDI /Fitzgerald
Cat# PRO16087 |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal rabbit
(antiserum, lyophilized) |
IF, IH(F, P) |
VAChT in both CNS and PNS |
rat, mouse |
VAChT
(poly)
IgG
|
Sigma Aldrich
Cat# V5387 |
immnuogen = synthetic peptide corr. to C-terminal of rat VAChT, conj. to KLH (aa 512-530, with N-terminally added lysine) |
polyclonal rabbit
(affinity isolated, PBS, BSA, azide) |
IB, IF, IH(P) |
VAChT; epitope highly conserved (>85% homology) in human VAChT |
human, rat |
VAChT
(poly)
IgY
|
US Biological
Cat #
V2121-86B |
immunogen = 20 aa peptide within the cytoplasmic, C-terminal domain of the rat/mouse VAT |
polyclonal chicken
(liquid, serum, PBS, azide, glycerol) |
ELISA, WB |
VAChT |
rat, mouse |
VAChT
(poly)
IgG
|
US Biological
Cat #
V2121-86A |
immunogen = synthetic peptide corresp. to the C-terminus of rat VAChT protein |
polyclonal guinea pig
(affinity purified, PBS, BSA, azide, lyophilized) |
IH, WB |
VAChT; CNS staining strong in fibers and cholinergic cell bodies |
rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAChT
(poly)
IgY
|
US Biological
Cat #
V2121-86D |
immunogen = 20aa peptide mapping within the C-terminus of rat/mouse VAT ( cytoplasmic), coupled to KLH |
polyclonal chicken
(liquid, PBS, BSA, azide, glycerol, purified) |
ELISA, WB |
VAChT |
mouse
(75% homology with human) |
VAChT
(poly)
|
US Biological
Cat #
V2121-85 |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal rabbit
(lyophilized, serum) |
IF, IH(F, P) |
VAChT in both CNS and PNS |
rat, mouse
(not human) |
VAChT
(poly)
IgG
|
US Biological
Cat #
V2121-85A |
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA |
polyclonal guinea pig
(lyophilized, serum) |
IH(F) |
VAChT in both CNS and PNS |
rat, mouse |
VAChT
(poly)
IgG
|
US Biological
Cat #
V2121-85B |
immunogen = synthetic peptide from the C-terminus of human VAChT conj. to BSA |
polyclonal rabbit
(lyophilized, serum) |
IH(F) |
VAChT in both CNS and PNS |
human |
VAChT
(poly)
|
Valeant Pharmaceutical International
Cat# 12004 |
|
polyclonal rabbit
|
|
VAChT |
human |
VAChT (C-20)
(poly)
IgG
|
Santa Cruz
Cat# sc-7716 |
immnuogen = peptide mapping at the C-terminus of human VAChT |
polyclonal goat
(affinity purified, PBS, azide, gelatin) |
IF, WB |
VAChT |
human, rat, mouse |
VAChT (H-160)
(poly)
IgG
|
Santa Cruz
Cat# sc-15315 |
immnuogen = protein corresp. to aa 251-410 mapping near C-terminus of human VAChT |
polyclonal rabbit
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
VAChT |
human, rat, mouse |
VAChT (N-19)
(poly)
IgG
|
Santa Cruz
Cat# sc-7717 |
immnuogen = peptide mapping at the N-terminus of human VAChT |
polyclonal goat
(affinity purified, PBS, azide, gelatin) |
IF, IP, WB |
VAChT |
human, rat, mouse |
VAT
(poly)
IgG
|
A D I
Cat# VAT11-S |
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT) |
polyclonal chicken
(neat serum, azide, available affinity purified) |
ELISA, IF, IH, WB |
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber |
rat, mouse
(others not tested) |
VAT
(poly)
Ig
|
Biogenesis AdB Serotec
Cat# 0030-5009 |
immunogen = synthetic 20 aa sequence from rat VAT protein, conj.with thyroglobulin |
polyclonal goat
(purified Ig, sepharose, BSA, PBS, azide) |
IF, IH, WB |
VAT; does not react with vesicular monoamine transporter (VMAT) |
rat, guinea pig, mouse
(not rabbit, cat or guinea pig) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAT
(poly)
Ig
|
Biogenesis AdB Serotec
Cat# 0030-5020 |
immunogen = sequence F-D-G-C-E-D- D-Y-N-Y-Y-S- R-S, conj. with KLH |
polyclonal sheep
(purified Ig, liquid, protein-G, BSA, PBS, azide) |
ELISA, IH, WB |
VAT; does not react with vesicular monoamine transporter (VMAT) |
rat |
VAT
(poly)
IgG
|
Biotrend
Cat#
VAT11-A
Datasheet available at Biotrend website |
immunogen = 20 aa mapping within C-terminal rat/mouse |
polyclonal chicken
(affinity purified antiserum) |
ELISA, IF, IH, WB |
VAChT |
rat, mouse
(93% homology with human and 91% with monkey) |
VAT
(poly)
IgG
|
Biotrend
Cat#
VAT11-S
datasheet available at Biotrend website |
immunogen = 20 aa mapping within C-terminal rat/mouse |
polyclonal chicken
(antiserum, neat) |
ELISA, IF, IH, WB |
VAChT |
rat, mouse
(93% homology with human and 91% with monkey) |
VAT
(poly)
Ig
|
Calbiochem EMD Biosciences
Cat #
PC178L |
immunogen = synthetic peptide corr. to aa 511-530 from cloned rat VAChT |
polyclonal goat
(antiserum, lyophilized) |
IF, IH(F) |
VAT; not vesicular monoamine transporters |
rat |
VAT
(poly)
IgG
|
Cambio
Cat#
CA-0185.609 |
|
polyclonal goat
(diluted serum) |
IF, WB |
VAT |
|
VAT
(poly)
IgG
|
Cambio
Cat#
CA-0185.620 |
|
polyclonal sheep
(Ig) |
ELISA, IH, WB |
VAT |
|
VAT
(poly)
|
CN Biosciences
Cat# PC-299 |
immunogen = synthetic peptide corresponding to amino acids 511-530 from cloned rat VAT |
polyclonal goat
(antiserum, liquid, azide) |
IF, IH(F) |
VAT. Not vesicular monoamine transporters. Cholinergic neurons in rat and mouse brain |
rat, guinea pig, mouse
(not feline, guinea pig or rabbit) |
VAT
(poly)
IgG
|
CN Biosciences
Cat# PC-178L |
immunogen = synthetic peptide corresponding to amino acids 511-530 from cloned rat VAT |
polyclonal goat
(antiserum, lyophilized) |
IF, IH(F) |
VAT. Not vesicular monoamine transporters. Positive reaction with rat basal forebrain |
rat |
VAT
(poly)
IgG
|
Gentaur
Cat# VAT11
produced by ADI |
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT) |
polyclonal chicken
(neat serum, azide, available affinity purified) |
ELISA, IF, IH, WB |
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber |
rat, mouse
(others not tested) |
VAT
(poly)
|
Immunostar
Cat #
24286 |
immunogen = synthetic peptide corresponding to aa 511-530 from the C-terminal of rat VAChT |
polyclonal goat
(whole serum, azide, lyophilized) |
IF, IH |
VAT; not vesicular monoamine transporters |
rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
VAT
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-B |
|
polyclonal goat
(diluted, serum) |
IF, WB |
VAT |
|
VAT
(poly)
|
ISL (Immune Systems Limited)
Cat# 126-4798 |
|
polyclonal sheep
(Ig) |
ELISA, IH, WB |
VAT |
|
VAT
(poly)
IgG
|
Novagen EMD Chemicals, Inc.
Cat #
PC178L
see Calbiochem |
immunogen = synthetic peptide corresponding to amino acids 511-530 from cloned rat VAT |
polyclonal goat
(antiserum, lyophilized) |
IF, IH(F) |
VAT. Not vesicular monoamine transporters. Positive reaction with rat basal forebrain |
rat |
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