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Home
:
Research
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Compendia
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Antibodies
Antibody Directory
Initial Search
Antigen Class:
-- all --
14-3-3
4E-BP1
4-hydroxy-nonenal
5-HT /serotonin
ABC, ABC transporter
ACC
ACE
Acetylcholine-related
Actin related
ADAM and ADAMTS
Adaptin
Adaptor Protein Complex (AP)
Adenomatus Polyposis Coli /APC
Adenylate cyclase
AFP
AGE and RAGE
Akt and serine/threonine kinases
Alix
Alpha-1-antitrypsin
α-, β-, γ-secretase
AMCase
Amino acids, phosphorylated
AMPAR, NMDAR, GluR1, GluR2
Amphiphysin
AMPK
Amyloid-beta related
Annexin II
AP-2 transcription factor
Apolipoproteins
APP, APLP
Arc
ASCL1/ASH1/MASH1
ASK
ATPase
Bad
Barrier-to-autointegration factor /BAF
Bassoon
Bax
Bcl and BAG
BDNF, pro-BDNF
β-Catenin related
β-galactosidase
Biotin
BiP and GRP78/KDEL
B-Lymphocyte
BrdU
BRSK
Bruchpilot
BSA
Cadherin
Calbindin & Calretinin
Calcium Channel
Calmodulin
Calnexin
Calpain
Calpastatin
Calponin
Calreticulin
calsyntenin
CaM Kinase II
Casein Kinase
CASK
Caspase
Cathepsin
Caveolin and GLUT
CD11b, Integrins
CD147
CD40L
CD45
CDK1/CDC2 and CDK2
CDK4, CDK5 and p25/35
CD_ except CD11b, CD40, CD45 and CD147
c-Fos and c-Jun
Chemokine receptor
CHIP
Charged multivesicular body protein /CHMP
Chromogranin
Chx
CI-MPR
Clathrin
CNPase
Cofilin
Collagen
Complement proteins
Connexin
COX1, COX2, COX3
Cre Recombinase
CREB, cAMP and CBP
CRF
Cyclins A, B, B1 and D2
CYP46A1
Cystatin C
Cytochrome C
DCC
DCP
DDX6 /RCK
Desmin and Vimentin
Digoxigenin
DJ-1
Dlg/Discs-large
Dlx2
DNA/RNA Oxidative Damage
Dopamine related: DBH, DRD, DDC
Doublecortin
DR6
Drebrin
Dynamin
Dynamin related protein
Dynein
eIF /Eukaryotic initiation factor
ECE
ECT, Lipid Metabolism
EEA1
EGF, EGFr
EGR-1 /Zif268
Elav proteins
Elongation Factors
Endosomes
ERAB
Erythroid cells
Exocyst Complex
F4/80
FADD
FAS-Related
F-box
Fe65
FGF, FGFR
Fibroblasts
FKBP12
FKHR/FoxO proteins
FLAG, ECS and other tags
Flotillin
Fragile-X /FMRP
Frizzled related
Furin Convertase
Futsch
GABA related and Gephyrin
GAD65/67
GalR, Substance P
Gangliosides
GAP related
GAPDH /G3PDH
GATA
GFAP
GFP and RFP
GITR
glucocorticoid receptor /GR
Glutamine Synthetase
Go
Golgi matrix/membrane, GM130
Grb-2
GRKs
GSK-3
GST
HA and HIS peptides
Hb9
Heat Shock Proteins
Heme Oxygenase, Peroxiredoxin
Heparan Sulfate, proteoglycans, MSRA
HES
Histone deacetylase (HDAC)
histones
hnRNP
Homer
Hox
Human nucleii
Huntingtin
Huntington's-related
Iba-1
IFN-γ
IGF Receptor
IgG
Insulin Receptor
Interleukins
IRF
iron metabolism: transferrin, ferroportin, CP
IRS
Islet
JAK2
JNK/SAPK-1/2/3 and MAPK8-10
Ki-67
Kinesin Heavy Chain
Lamin and Nuclear Pore Complex
Laminin
Lamp
Lck and Lck Ligand
LDL Receptor family
LHX2
LL37 /CAP18
LRRK2
LTE4
Ly-6 surface proteins
MAPK, ERK
MAPKAPK/MAPK activated kinase
MAPs
Matrix Metalloproteinase (MMP)
MeCP2
MEK/MAP2K
MEKK/MAP3K and MAPKAP1
MEKKK/MAP4K
Melanocyte Stimulating Hormone
Met-Enkephalin
MHC related proteins
Mint
Mitochondria related
MKK
Monocytes
mTOR-related
myc and V5 epitope tags
Myelin Basic Protein
Myocardin
Myosin
Nanog
NCP1
NDUFS4
Neprilysin /CD10
Nestin, Neural Progenitors
NeuN
Neurexin
Neuro D
Neurofib Tangles and p62
Neurofilament
Neurogenin
Neuropeptide Y
Neurophysin Related
NFkB, IkB and related proteins
NG2
NGF, NGF-related
NGFR, Trk-A/B
Nieman Pick C1
Nitrotyrosine
Nkx
NOS
Notch, Jagged, Delta
NOX2
NSE
NUMB and NUMBLike
Occludin
O-GlcNAc
Olfactory Proteins
Oligodendrocyte markers
Optineurin
Orexin
p19
p21 and p27
p38 MAPK and MAPK11-14
p53
PAK
PARP
Parvalbumin
PAX6
PCAF
PCNA
PCTAIRE
PDGF, PDGF Receptor
PGC
Phosphorylated Strings
PIAS3
PINK1
PIP
PKA, PKC and related
Plaque-related
Potassium channel, voltage gated
Presenilin Complex, Nicastrin, Ubiquilin
Presenilins
Prion protein
Progranulin /PCDGF
Proline disulfide isomerase /PDI
Proteasome related
Protein Phosphatase related
proteolipid protein /PLP
PSA-NCAM
PSD95
PTPs
Rab or Ras related, except Rab3
Rac and Cdc42
RAP
Recoverin
Reelin
Retrolinkin
Retromer Complex
Rho
Rhodopsin
Ribonucleotide Reductase
Ryanodine receptor
S-100
S6 Kinase
Sestrin
SHP2
SIRT1/Sir2 and SIRT2
SNX
SOCS3/CIS3
SOD
Sodium channel, voltage gated
Somatostatin related
Sortilins
SOS-1
Sox
Spectrins
Splicing Factors
SR-BI
Src
SREBP
SRF
SSEA
Stat1, Stat3 and related proteins
Sulfiredoxin
SUMO
Synaptic Vesicle proteins
Synaptosomal proteins
Synucleins
TACE
TAR DNA-binding protein /TDP-43
Tau
Tbr
TdT
TGF
Thioredoxin related
TIA related
Tip60
TLR /Toll-like Receptor
TMP21
TNF
Tom
tPA and PAI-1
TRA-1
Trans-Golgi network /TGN
TRRAP
Tryptophan Hydroxylase
TSG101
Tubulin
TUC (TOAD/Ulip/CRMP)
Tyrosine Hydroxylase
Tyrosine kinase
UbcH
Ubiquitin
Urocortin
VAChT
Vav
VCAM
VDAC /Porin
VEGF and VEGF Receptor
Veli
VGAT
vGluT
von Willebrand factor
Vps35
WNT3
ZO-1
(plus)
Keywords:
any keywords
all keywords
Search Results:
Antigen Class
=Ubiquitin
Narrow Results
Host:
Method:
Species Reactivity:
Distributor:
-- select --
chicken
goat
mouse
rabbit
rat
sheep
----------------
all monoclonal
all polyclonal
all kit
-- select --
DB
ELISA
EM
FC
IA
IB
IC
IEP
IF
IH
IH(F)
IH(P)
IH(V)
IP
RID
WB
and
-- select --
Arabidopsis
Arabidopsis thaliana
baboon
bovine
C. elegans
chicken
cow
dog
drosophila
E. Coli
equine
fish (various)
frog
guinea pig
hamster
horse
human
mammal (various)
monkey
mouse
nematode
non-specific
plant (various)
primate (various)
rabbit
rat
S. cerevisiae
sheep
various species
wistar rat
yeast
and
-- select --
Abcam Ltd
AnaSpec, Inc
Biogenesis (AbD Sero
biømeda
BIOMOL Enzo Life Sci
BIOTREND Chemikalien
CHEMICON INTERNATION
Covance Research Pro
Dako
EnCor Biotechnology,
Fitzgerald Industrie
KAMIYA BIOMEDICAL CO
MBL International
Novus Biologicals, I
PharMingen BD Biosci
R&D Systems
RDI --Division of Fi
SANTA CRUZ BIOTECHNO
Serotec Inc
SIGMA-ALDRICH U.S.
SIGNET Covance
Stressgen - Enzo Lif
ZYMED Invitrogen Cor
exclude selected host
-- select --
DB
ELISA
EM
FC
IA
IB
IC
IEP
IF
IH
IH(F)
IH(P)
IH(V)
IP
RID
WB
-- select --
Arabidopsis
Arabidopsis thaliana
baboon
bovine
C. elegans
chicken
cow
dog
drosophila
E. Coli
equine
fish (various)
frog
guinea pig
hamster
horse
human
mammal (various)
monkey
mouse
nematode
non-specific
plant (various)
primate (various)
rabbit
rat
S. cerevisiae
sheep
various species
wistar rat
yeast
1 to 100 of 116 results
Important Notice:
We are striving to include as many companies or products as possible, but we do not make a claim to being comprehensive. We may inadvertently omit specific companies or products due to a lack of information.
Antibodies to Ubiquitin Proteins
From the Laboratories
Antigen
(Clone)
IgG
Name
(Source)
used in
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin+1, frameshift mutant
(mono)
(Sungmin Song, Department of Life Science, Kwangju Institute of Science and Technology, Kwangju, Korea)
Paper
Reference:
Ko, S, 2010
immunogen = synthetic peptide of the same amino acid sequence as previously described in
van Leeuwen et al.
, 1998
monoclonal rat
(liquid, affinity purified)
IC, IH, WB
reacts with Ubiquitin+1 and
not
Ubiquitin
human
Ubiquitin+1, frameshift mutant
(poly)
Ubi-B
1
and Ubi-B
2
(
Fred W. van Leeuwen
, Netherlands Institute for Brain Research, Amsterdam, Netherlands)
Paper
immunogen = synthetic peptides covering YADLRED PDRQDHH PGSGAQ at the C-terminus of Ubiquitin+1
polyclonal rabbit
(See also R&D systems antibodies MAB703 andAF703)
IB, IC, IH
reacts with Ubiquitin+1 and
not
Ubiquitin
human
From the Manufacturers
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
UbcH10
(poly)
Chemicon and Upstate are Millipore
Cat# AB3861
immunogen = full length, highly purified, UbcH10 protein
polyclonal rabbit
(liquid, protein-A purified, PBS, glycerol, azide)
IP, WB
UbcH10
human
UbcH2
(poly)
Chemicon and Upstate are Millipore
Cat# AB3855
immunogen = full length, highly purified, UbcH2 protein
polyclonal rabbit
(liquid, protein-A purified, PBS, glycerol, azide)
IP, WB
UbcH2
human
UbcH6
(poly)
Chemicon and Upstate are Millipore
Cat# AB3858
immunogen = full length, highly purified, UbcH6 protein
polyclonal rabbit
(liquid, protein-A purified, PBS, glycerol, azide)
IP, WB
UbcH6
human
UbcH7
(poly)
Chemicon and Upstate are Millipore
Cat# AB3859
immunogen = full length, highly purified, UbcH7 protein
polyclonal rabbit
(liquid, protein-A purified, PBS, glycerol, azide)
IP, WB
UbcH7
human
UbcH9
(poly)
Chemicon and Upstate are Millipore
Cat# AB3860
Discontinued
immunogen = full length, highly purified, UbcH9 protein
polyclonal rabbit
(liquid, protein-A purified, PBS, glycerol, azide)
IP, WB
UbcH9
human
Ubiquitin
(10C2-2)
IgG
1
,κ
Abcam
Cat# AB411
monoclonal mouse
(supernate, no preservative, lyophilized)
ELISA,
IH(F, P)
, WB; AP
reacts specifically with 8.5 kDa protein and ubiquitinated proteins
Ubiquitin
(1B4-UB)
IgG
1
Abcam
Cat# ab122
immunogen = ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin
monoclonal mouse
(liquid, IgG fraction, PBS, azide)
ELISA, FC,
IH(F, P)
, WB
ubiquitin
human, cow
(others not tested)
Ubiquitin
(EP296YT)
IgG
Abcam
Cat# ab33893
immunogen = KLH-synthetic peptide corrsp. to residues in the C-terminal of human Ubiquitin
monoclonal rabbit
(liquid, IgG fraction, glycerol, BSA, Tris, azide)
IH(P)
, WB;
not
FACS, ICC/IF or IP
ubiquitin
human, rat, mouse
(others not tested)
Ubiquitin
(P4G7)
IgG
1
Abcam
Cat# ab24686
immunogen = full length denatured protein (purified) (Cow)
monoclonal mouse
(liquid, ascites, no preservative)
ELISA, WB
ubiquitin
(expect wide range of species-yeast to human)
Ubiquitin
(PD41)
IgG
Abcam
Cat# ab34490
immunogen = full length ubiquitin of bovine origin
monoclonal mouse
(liquid, HEPES, BSA, NaCl, glycerol, protein A purified)
IH(P, V)
, WB
ubiquitin
human, primate (various), rat, mouse, cow
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(Ubi-1)
IgG
1
,κ
Abcam
Cat# ab7254
immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker
monoclonal mouse
(liquid, ascites, azide)
IH(F, P)
, IP, WB
recognizes polyubiquitin chains much more strongly than mono ubiquitinated molecules or free ubiquitin; specifically recognizes ubiquitinated cytoplasmic inclusion bodies
human, mouse, cow, Arabidopsis thaliana, drosophila, C. elegans, chicken
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab837
immunogen = ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins
polyclonal rabbit
(liquid, protein-A purified)
IF,
IH(F, P)
ubiquitin; also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer disease
human, rat, mouse, cow
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab6181
immunogen = Ubiquitin from Sigma (Product number U6253) that was coupled to chicken gammaglobulin
polyclonal sheep
(liquid, whole antiserum)
DB,
IH(F)
, WB
both free ubiquitin and ubiquitin protein conjugates; labels tangles and Lewy bodies
human, rabbit, sheep
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab6309
immunogen = bovine red blood cell ubiquitin conjugated to KLH
polyclonal rabbit
(liquid, whole antiserum, PBS, lactose, no preservative)
IH(F)
, WB
ubiquitin
cow, S. cerevisiae
(others not tested, expect wide range)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab7780
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R
polyclonal rabbit
(liquid, whole antiserum, tris, carriers, azide)
ELISA,
IH(F, P)
, IP, WB
reacts with physiologically occurring conjugates of ubiquitin and intracellular proteins; specifically recognizes ubiquitinated cytoplasmic inclusion bodies
human, rat, mouse, horse, cow
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# AB8134
immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker
polyclonal rabbit
(liquid, whole antiserum)
ELISA, IF,
IH(F, P)
, IP, WB
recognizes ubiquitinated inclusion bodies
human, cow, drosophila, C. elegans, chicken
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab8546
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R
polyclonal rabbit
(liquid, whole antiserum, green food dye, azide, predilute)
IH(P)
ubiquitin; periphery of senile plaques, neurofibrillary tangles in AD brain, Lewy bodies in Parkinson's; mallory bodies in alcoholic liver disease
human, rat, mouse, horse, cow
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab19247
immunogen = full length native protein, purified from bovine red blood cells
polyclonal rabbit
(liquid, whole serum, no preservative)
IH, IP, WB
free ubiquitin as well as ubiquinated proteins
various species
Ubiquitin
(poly)
IgG
Abcam
Cat# ab27268
immunogen = recomb. full length human protein
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide)
IH(P)
ubiquitin
human
(others not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab38633
immunogen = KLH-conjugated synthetic peptide selected within aa 1-100 of human Ubiquitin
polyclonal rabbit
(liquid, protein-G purified, PBS, azide)
ELISA,
IH(P)
, WB
ubiquitin, aminoterminal end
human
(others not tested)
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(poly)
IgG
Abcam
Cat# ab14372
immunogen = ubiquitin coupled to rabbit IgG
polyclonal rabbit
(liquid, KPO4-saline, azide, purified)
ELISA, WB
ubiquitin
yeast
Ubiquitin
(poly)
IgG
Abcam
Cat# ab35591
immunogen = purified ubiquitin purified from bovine red blood cells
polyclonal sheep
(liquid, PBS, azide, purified)
ELISA
ubiquitin
cow
(expect wide range of species)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab31776
immunogen = highly purified ubiquitin from bovine erythrocytes
polyclonal sheep
(liquid, PBS, azide, purified)
ELISA
ubiquitin
cow
(expect wide range of species due to seq. homology)
Ubiquitin
(poly)
IgY
Abcam
Cat# ab19310
immunogen = recombinant full length human protein
polyclonal chicken
(liquid, PBS, azide, affinity purified)
WB
ubiquitin
human
Ubiquitin
(poly)
IgG
Abcam
Cat# ab19168
immunogen = commercially sensitive
polyclonal rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified)
WB
epitope = maps to a region between residues 1-50 of human ubiquitin; Swiss-Prot entry P62988
human, cow
(expect all members fo kingdom Animalia due to seq. homology)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab19169
immunogen = commercially sensitive
polyclonal rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified)
WB
ubiquitin
plant (various)
(other species not tested)
Ubiquitin
(poly)
IgG
Abcam
Cat# ab35227
immunogen = full length native protein from rbc's (cow)
polyclonal sheep
(liquid, glycerol, PBS, MgCl, ZnCl, azide, protein G purified)
ELISA, IA, WB
ubiquitin
cow
(expect wide range of species due to seq. homology)
Ubiquitin
(poly)
AnaSpec
Cat# 53934
immunogen = a recombinant full length human ubiquitin protein
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide)
IH(P)
detects Ubiquitin
human
Ubiquitin
(242.9)
IgG
1
Biogenesis AdB Serotec
Cat# 9400-1004
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde
monoclonal mouse
(liquid, ascites, azide)
EM,
IH(F, P)
, WB
Ubiquitin; tangles in AD brain, Lewy bodies, Pick bodies
human
Ubiquitin
(poly)
Biogenesis AdB Serotec
Cat# 9400-0000
immunogen = ubiquitin from bovine erythrocytes
polyclonal rabbit
(purified IgG, PBS, stabilizers)
IH(F, P)
ubiquitin
human, rat, mouse, cow
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(poly)
Biogenesis AdB Serotec
Cat# 9400-0039
immunogen = purified bovine ubiquitin
polyclonal rabbit
(serum, lyophilized)
RID, WB
ubiquitin
Ubiquitin
(poly)
Biogenesis AdB Serotec
Cat# 9400-0409
immunogen = ubiquitin from bovine RBCs
polyclonal sheep
(neat serum, filtered, azide)
ELISA, IH, WB
Ubiquitin
various species
Ubiquitin
(poly)
Biomeda
Cat# 221M
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R
polyclonal rabbit
(Tris, carriers, azide, buffered, prediluted)
IH(F, P)
8.5kDa ubiquitin
human, rat, mouse, equine, bovine
(others not tested)
Ubiquitin
(poly)
Biomeda
Cat# V2015
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R
polyclonal rabbit
(Tris, carriers, azide, concentrated)
IH(F, P)
8.5kDa ubiquitin; nuclear, cytoplasmic and cell membrane
human, rat, mouse, equine, bovine
(others not tested)
Ubiquitin
(kit)
Biomeda
Cat# D221M
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R
rabbit
(Tris, carriers, azide, prediluted -staining kit)
IH(F, P)
8.5kDa ubiquitin
human, rat, mouse, equine, bovine
(others not tested)
Ubiquitin
(FK1)
IgM
Biomol Enzo Life Sciences
Cat# PW 8805
Reference:
Tsuda, 2008
immunogen = poly-ubiquitinylated- lysozyme
monoclonal mouse
(liquid, purified Ig, PBS, azide)
IH, WB
recognises only poly ubiquitinylated proteins and not mono ubiquitinylated proteins or free ubiquitin
Ubiquitin
(FK2)
IgG
1
Biomol Enzo Life Sciences
Cat# PW 8810
Reference:
Ratnaparkhi, 2008
immunogen = crude preparation of poly ubiquitinylated- lysozyme
monoclonal mouse
(liquid, purified Ig, PBS saline, azide)
ELISA, IF, IP, WB
recognises mono and poly ubiquitinylated proteins but not free ubiquitin
non-specific
Ubiquitin
(242.9)
IgG
1
Biotrend
Cat# 9400-1004
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde
monoclonal mouse
(liquid, ascites, azide)
EM,
IH(F, P)
, WB
Ubiquitin
human
Ubiquitin
(poly)
Biotrend
Cat# 9400-0000
immunogen = ubiquitin from bovine erythrocytes
polyclonal rabbit
(purified IgG, PBS)
IH(F, P)
ubiquitin
human, rat, mouse, cow
Ubiquitin
(poly)
Biotrend
Cat# 9400-0039
immunogen = purified bovine ubiquitin
polyclonal rabbit
(serum, lyophilized)
RID, WB
ubiquitin
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(poly)
Biotrend
Cat# 9400-0409
immunogen = ubiquitin from bovine RBCs
polyclonal sheep
(neat serum, filtered, azide)
ELISA, IH, WB
Ubiquitin
various species
Ubiquitin
(poly)
Biotrend
Cat# PDR010
ubiquitin isolated from cow euythrocytes
polyclonal rabbit
(pre-titer)
IH
periphery of senile plaques and neurofibrillay tangles in AD
human, mammal (various)
Ubiquitin
(poly)
Biotrend
Cat# PDR040
ubiquitin isolated from cow euythrocytes
polyclonal rabbit
(serum Ig, purified, azide)
IH
periphery of senile plaques and neurofibrillay tangles in AD
human, mammal (various)
Ubiquitin
(Ubi-1)
IgG
1
Chemicon and Upstate are Millipore
Cat# MAB1510
References:
Emmanouilidou, 2010
Wegorzewska, 2009
Neumann, 2006
Giasson, 2002
immunogen = purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde
monoclonal mouse
(liquid, ascites, azide)
IH(F, P)
, WB
ubiquitin, paired helical filaments (PHF) of AD, Pick body and Lewy body
human
Ubiquitin
(poly)
Chemicon and Upstate are Millipore
Cat# AB1586
immunogen = ubiquitin from bovine red blood cells coupled to chicken gamma globulin
polyclonal sheep
(serum, no preservative, lyophilized)
IH, WB
free ubiquitin and ubiquitin-protein conjugates, tangles and Lewy bodies
human, rabbit, sheep
Ubiquitin
(poly)
Chemicon and Upstate are Millipore
Cat# AB1690
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde
polyclonal rabbit
(serum, azide)
IH, WB
ubiqiutin in neurofibrillary tangles, neurites in senile plaques. No reactivity seen to tau, neurofilament or MAP.
Ubiquitin
(poly)
Chemicon and Upstate are Millipore
Cat# AB5621
immunogen = full length human ubiquitin
polyclonal chicken
(liquid, affinity purified IgG, PBS)
ELISA, IC, IH, WB; expect IC
Ubiquitin
human
Ubiquitin
(P4D1)
IgG
1
Covance Research Products, Inc.
cat# MMS-257P
immunogen = denatured bovine ubiquitin
monoclonal mouse
(purified)
ELISA, WB
recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins
human, yeast, various species
Ubiquitin
(P4G7)
IgG
1
Covance Research Products, Inc.
cat# MMS-258R
immunogen = denatured bovine ubiquitin
monoclonal mouse
(ascites)
WB
recognizes ubiquitin, polyubiquitin, and ubiquitin conjugated proteins
human, yeast, various species
Ubiquitin
(Ubi-1)
IgG
1
,κ
Covance Research Products, Inc.
cat# MMS-264R
immunogen = purified KLH-conjugated ubiquitin
monoclonal mouse
(ascites, azide)
ELISA, IH, WB
ubiquitin; insensitive to formalin fixation, neurofibrillary tangles, Lewy bodies of PD and the Pick bodies in Pick's disease
human, bovine, drosophila, nematode, chicken
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(poly)
IgG
1
Covance Research Products, Inc.
cat# PRB-268C
immunogen = glutaraldehyde cross linked ubiquitin
polyclonal rabbit
(serum)
IF, WB
recognizes ubiquitin
human
Ubiquitin
(poly)
Dako
cat# Z0458
References:
Machiya, 2010
Gijselinck, 2010
Houlden, 2008
Josephs, 2007
Brouwers, 2007
Komatsu, 2006
Neumann, 2006
immunogen = Ubiquitin isolated from cow erythrocytes and conj. to chicken gamma globulins with glutaraldehyde
polyclonal rabbit
(purified Ig fraction, saline, azide)
ELISA, IC, IEP, WB
periphery of senile plaques and of neurofibrillary tangles, Lewy bodies, Mallory bodies
human, baboon, wistar rat
Ubiquitin
(Ubi-1)
IgG
1
κ
EnCor Biotechnology, Inc,
cat# MCA-Ubi-1
purified ubiquitin conj. with glutaraldehyde to keyhole limpet hemocyanin
monoclonal mouse
(ascites, purified, BSA, KLH)
IF, IH, WB
Neurofibrillary tangles strongly and specifically; also recognizes ubiquitinated inclusion bodiess such as lewey and pick bodies
human, bovine, drosophila, C. elegans, chicken
Ubiquitin
(poly)
Ig
EnCor Biotechnology, Inc,
cat# RPCA-Ubi
glutaraldehyde cross linked ubiquitin in rabbit
polyclonal rabbit
(crude serum)
IF, WB
Ubiquitinated inclusion bodies i.e.neurofibrillary tangles of AD, Lewy bodies, Pick bodies
Ubiquitin
(poly)
Fitzgerald
Cat# 20-UR20
immunogen = bovine ubiquitin
polyclonal rabbit
(serum, azide)
IH, WB
ubiquitin, especially Lewy bodies in Parkinson disease.
Ubiquitin
(KM691)
IgM
Kamiya
Cat# MC-033
immunogen = recomb. human ubiquitin
monoclonal mouse
(liquid, ascites, azide)
IH, WB
ubiquitin
human
Ubiquitin
(1B3)
IgG
1
MBL International
Cat#
MK11-3
reference
Imai, 2001
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(affinity purified, lyophilized, PBS, no preserves)
IH, WB
reacts with erythrocyte ubiquitin
human, mouse, bovine
Ubiquitin
(2C5)
IgG
1
MBL International
Cat#
MK12-3
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(affinity purified, lyophilized, PBS, no preserves)
WB
reacts with erythrocyte ubiquitin
human, mouse, bovine
Ubiquitin
(10C2-2)
IgG
1
,κ
Novus Biologicals
Cat# NB100-1650
monoclonal mouse
(lyophilized, supernatant, no preservative)
ELISA,
IH(F, P)
, IP, WB
ubiquitin proteins (8.5kDa); cellular and nuclear
bovine
Ubiquitin
(1B4-UB)
IgG
1
Novus Biologicals
Cat# NB600-776
immunogen = ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin
monoclonal mouse
(liquid, PBS, azide)
FC,
IH(F, P)
ubiquitin
human, cow
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(Ubi-1)
IgG
1
,κ
Novus Biologicals
Cat# NB300-130
immunogen = purified ubiquitin conjugated to KLH with glutaraldehyde
monoclonal mouse
(liquid, ascites, azide)
ELISA,
IH(F, P)
, WB
recognizes ubiquitinated cytoplasmic inclusion bodies
human, mouse, bovine, Arabidopsis, drosophila, C. elegans, chicken
Ubiquitin
(poly)
IgG
Novus Biologicals
Cat# NB600-473
immunogen = ubiquitin coupled to rabbit IgG
polyclonal rabbit
(liquid, KPBS, azide, purified)
ELISA, WB
ubiquitin
yeast
Ubiquitin
(poly)
Novus Biologicals
Cat# NB300-262
immunogen = synthetic peptide mapping to a region between residues 1-50 of soybean Ubiquitin; Swiss-Prot entry P03993
polyclonal rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified)
WB
ubiquitin
plant (various)
Ubiquitin
(poly)
Novus Biologicals
Cat# NB300-263
immunogen = synthetic peptide mapping to a region between residues 1-50 of human Ubiquitin; Swiss-Prot entry P62988.
polyclonal rabbit
(liquid, tris-citrate/phosphate, azide)
WB
ubiquitin
human, bovine
(expect all members of Kingdom Animalia due to seq. homology)
Ubiquitin
(poly)
Novus Biologicals
Cat# NB300-129
immunogen = gluteraldehyde cross-linked ubiquitin.
polyclonal rabbit
(liquid, sera)
ELISA, IF, IH, WB
recognizes ubiquitinated inclusion bodies and both the mono- and polyubiquitin forms; also aggresomes
human, mouse, bovine, drosophila, C. elegans, chicken
Ubiquitin
(poly)
IgG
Novus Biologicals
Cat# NB120-837
immunogen = ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins
polyclonal rabbit
(liquid, protein-A purified)
IF,
IH(F, P)
ubiquitin; also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer disease
human, rat, mouse, cow
(others not tested)
Ubiquitin
(6C1.17)
IgG
1
BD Pharmingen
Cat# 550944
immunogen = bovine erythrocyte ubiquitin was coupled to ovalbumin with glutaraldehyde
monoclonal mouse
(ascites, PBS, azide)
WB
recognizes human ubiquitinated proteins
human
(expect other species because of the highly conserved nature of this molecule)
Ubiquitin
(1B3)
IgG
1
RDI /Fitzgerald
Cat# UBIQ-1B3
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(ascites, affinity purified, azide)
FC, WB
human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda bySDS-PAGE
human, bovine
Ubiquitin
(2C5)
IgG
1
RDI /Fitzgerald
Cat# UBIQ-2C5
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(liquid, ascites, affinity purified, azide)
FC, IH, WB
human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda bySDS-PAGE
human, bovine
Ubiquitin
(FPM1)
IgG
1
RDI /Fitzgerald
Cat# UBIQUITabm
immunogen = ubiquitin conjugated with glutaraldehyde cross linked to KLH
monoclonal mouse
(supernate, azide, lyophilized)
FC, IH, WB
reacts to brain sections (tangles) from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(P1A6)
IgG
1
RDI /Fitzgerald
Cat# UBIQNabmX
aa 1-46 of the amino terminus of ubiquitinof bovine origin
monoclonal mouse
FC, IH, WB
ubiquitin, poly-ubiquitin and ubiquitinated proteins
human, rat, mouse, various species
Ubiquitin
(poly)
IgG
RDI /Fitzgerald
Cat# UBIQNabg
(aa 2-20 of the carboxy terminus of ubiquitin of human origin)
polyclonal goat
IH, WB
ubiquitin, neurofibrillary tangles in Alzheimer disease, Lewy bodies
human, rat, mouse
Ubiquitin
(poly)
RDI /Fitzgerald
Cat# UBIQUITabR
immunogen = ubiquitin conjugated with glutaraldehyde cross linked to KLH
polyclonal rabbit
(serum, azide, lyophilized)
IH(F, P)
, WB
reacts to brain sections (tangles) from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity
human, rat, mouse, cow
Ubiquitin
(poly)
RDI /Fitzgerald
Cat# UBQITNabR-BL
immunogen = ubiquitin from bovine red blood cells conjugated to KLH
polyclonal rabbit
(serum, prediluted, lyophilized)
IH, WB
detects neurofibrillary tangles in Alzheimer's disease, Lewy bodies in Lewy Body disease; also dot-like structures in the white matter of normal aging brain
human
Ubiquitin
(10C2-2)
IgG
1
Santa Cruz
Cat# sc-58448
immunogen = Ubiquitin from red blood cells of cow origin
monoclonal mouse
(liquid, supernatant, IgG, azide)
IF,
IH(P)
, WB
detects ubiquitin, 8.5 kDa in WB
human, rat, mouse
(expect cow, other mamalian)
Ubiquitin
(1B4-UB)
IgG
1
Santa Cruz
Cat# sc-58449
immunogen = Ub from cow erythrocytes conjugated to bovine gammaglobulin
monoclonal mouse
(liquid, PBS, azide, gelatin)
FC, IF,
IH(P)
, IP, WB
detects ubiquitin
human, cow
Ubiquitin
(6C1)
IgG
2a
Santa Cruz
Cat# sc-47721
immunogen = cow erythrocyte ubiquitin
monoclonal mouse
(liquid, azide)
IP, WB
detects ubiquitin
non-specific
Ubiquitin
(98.54)
IgG
2a
Santa Cruz
Cat# sc-130410
immunogen = full length recombinant Ub of human origin
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF,
IH(P)
, IP, WB
detects ubiquitin
human
Ubiquitin
(A-5)
IgG
2b
Santa Cruz
Cat# sc-166553
immunogen = amino acids 1-76 representing full length Ub of human origin.
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
detects ubiquitin and polyubiquitin
human, rat, mouse
Ubiquitin
(F-11)
IgG
1
Santa Cruz
Cat# sc-271289
immunogen = amino acids 1-76 representing full length Ub of human origin.
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB
detects ubiquitin and polyubiquitin
human, rat, mouse
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(P4D1)
IgG
1
Santa Cruz
Cat#
sc-8017
References:
Zhang, 2008
Tatebayashi, 2002
Tanemura, 2006
epitope corr. to aa 1-76 representing full length bovine Ubiquitin
monoclonal mouse
(liquid, PBS, azide, gelatin
Also available as agarose, HRP or FITC conj. and as Alexa Fluor®)
IF,
IH(P)
, IP, WB
detects ubiquitin, poly-ubiquitin and ubiquitinated proteins
human, rat, mouse, drosophila
Ubiquitin
(P4G7)
IgG
1
Santa Cruz
Cat# sc-53509
immunogen = full length denatured ubiquitin of bovine origin
monoclonal mouse
(liquid, PBS, azide, gelatin)
ELISA, IP, WB
detects ubiquitin, polyubiquitin and ubiquitin conjugated proteins
human, rat, mouse, cow, yeast
Ubiquitin
(Ubi-1)
IgG
1
Santa Cruz
Cat# sc-52750
immunogen = purified ubiquitin of cow origin
monoclonal mouse
(liquid, ascites, azide)
IF,
IH(P)
, IP, WB
detects ubiquitin
human, mouse, cow, drosophila, C. elegans
Ubiquitin
(1B4-UB)
IgG
1
AbD Serotec
Cat# MCA1398GT
immunogen = ubiquitin from bovine erythrocytes conj. to bovine gamma globulin
monoclonal mouse
(liquid, IgG fraction, PBS, azide, protein G purified)
FC,
IH(F, P)
ubiquitin
human, bovine
Ubiquitin
(1B4-UB)
IgG
1
AbD Serotec
Cat# MCA1398G
immunogen = ubiquitin from bovine erythrocytes conj. to bovine gamma globulin
monoclonal mouse
(liquid, IgG fraction, PBS, azide, protein G purified)
ELISA, FC,
IH(F, P)
, WB
ubiquitin
human, bovine
Ubiquitin
(242.9)
IgG
1
AbD Serotec
Cat# 9400-1004
immunogen = synthetic peptide corresp. to part of the native molecule conjugated to KLH
monoclonal mouse
(liquid, ascites, azide)
IH(F, P)
, WB
ubiquitin (76 aa, 8.5 kDa); stains tangle material in Alzheimer brain; also stains Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease).
bovine
Ubiquitin
(poly)
IgG
1
AbD Serotec
Cat# 9400-0419
immunogen = native, purified >= 90% from bovine erythrocytes
polyclonal sheep
(liquid, PBS, azide, NH2SO4 frac.)
ELISA
ubiquitin (76 aa, 8.5 kDa); stains tangle material in Alzheimer brain; also stains Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease).
bovine, various species
Ubiquitin
(6C1)
IgG
2a
Sigma Aldrich
Cat# U0508
immunogen = purified bovine ubiquitin conj. to KLH
monoclonal mouse
(liquid, ascites, azide)
ELISA, WB
ubiquitin
human, rat, mouse, bovine
Ubiquitin
(poly)
Sigma Aldrich
Cat# U5379
References:
Maruyama, H, 2010
Ihara, 2007
Caldwell, 2003
immunogen = bovine red blood cell ubiquitin conj. to KLH
polyclonal rabbit
(serum, PBS, predilute, no preservative, lyophilized)
IH, WB
ubiquitin
various species
(prokaryotes, eukaryotes)
Ubiquitin
(3-39)
IgG
1
κ
Signet Covance
Cat# 9500
monoclonal mouse
(ascites)
ELISA, IH, IP, WB
residues 50-65 of ubiquitin in PHF,
not
free ubiquitin. Highly reactive on formalin fixed tissues.
Antigen
(Clone)
IgG
Distributor
(Producer)
Immunogen
(Epitope)
Host
(Formulation)
Methods
glossary
Specificity
Reactivity
top
Ubiquitin
(5-25)
IgG
1
κ
Signet Covance
Cat# 9400
monoclonal mouse
(ascites)
ELISA, IH, IP, WB
residues 64-76 of ubiquitin and ubiquitin in PHF. Formalin fixation eliminates reactivity.
Ubiquitin
(1B3)
IgG
1
Stressgen Enzo Life Sciences
Cat# SPA-201
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(protein-A, PBS, no preserves, lyophilized)
IH, WB
~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-202
human, bovine
Ubiquitin
(2C5)
IgG
1
Stressgen Enzo Life Sciences
Cat# SPA-202
immunogen = purified bovine erythrocyte ubiquitin
monoclonal mouse
(protein-A, PBS, no preserves, lyophilized)
WB
~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-201
human, bovine
Ubiquitin
(FK2)
IgG
1
Stressgen Enzo Life Sciences
Cat# SPA-205
immunogen = crude poly ubiquitin lysozyme
monoclonal mouse
(protein-A, Ig, PBS, glycerol)
IH, WB
ubiquitinated proteins;
not
free ubiquitin
Ubiquitin
(p4G7-H11)
IgG
Stressgen Enzo Life Sciences
Cat# SPA-203
immunogen = natural source bovine ubiquitin
monoclonal mouse
(Ig, protein-G, PBS, PMSF)
ELISA, WB
both mono and poly ubiquinated proteins
human, rat, guinea pig, mouse, hamster, rabbit, sheep, dog, bovine, yeast, drosophila, chicken
Ubiquitin
(poly)
Stressgen Enzo Life Sciences
Cat# SPA-200
reference
(rabbit poly)
Yoo, 2003
immunogen = ubiquitin purified from bovine red blood cells, conj. to KLH
polyclonal rabbit
(whole serum)
IH, IP, WB
free ubiquitin and ubiquinated proteins; will immuno- precipitate BSA-conjugated ubiquitin
monkey, human, rat, mouse, hamster, rabbit, sheep, dog, bovine, yeast, E. Coli, drosophila, fish (various), frog, chicken
Ubiquitin
(UBI-1)
IgG
1
κ
Zymed/ Invitrogen
Cat# 13-1600
reference
(mono ubiquitn)
Finley, 2003
immunogen = purified bovine ubiquitin conj. to carrier protein
monoclonal mouse
(liquid, PBS, azide)
ELISA, IF, IH, IP, WB
ubiquitin, both conjugated and unconjugated; reacts with single chain 8.5 kDa protein
human, mouse, bovine, drosophila, C. elegans, chicken
Ubiquitin
(Ubi-1)
IgG
1
κ
Zymed/ Invitrogen
Cat# 08-0147
reference
Holbert, 2003
immunogen = bovine ubiquitin
monoclonal mouse
(liquid, purified ascites, PBS, BSA, azide, predilute)
IH(F, P)
ubiquitin; reacts with single chain 8.5 kDa protein
human
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