|
Antibodies to Transforming Growth Factor (TGF)-related Antigens
|
| From the Manufacturers
|
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
ARTS
(ARTS51)
IgG2b
|
Sigma Aldrich
Cat# T4471 |
immunogen = synthetic peptide corr. to C-terminus residues (aa 247-266) of human ARTS protein, conj. to KLH. |
monoclonal mouse
(liquid, Ig fract, purified, PBS, azide) |
ELISA, IB, IC |
32 kDa apoptosis related protein in the TGF-β signaling pathway (ARTS) |
human |
ARTS
(poly)
IgG
|
US Biological
Cat #
A3595 |
immunogen = synthetic peptide (K R F L E D T T D D G E) corresp. to aa 3 to 14 of human ARTS |
polyclonal rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
ARTS (apoptosis related protein in TGFβ signaling pathway), 32kDa |
human |
CD105/ Endoglin
(8E11)
IgM
|
Biodesign Meridian LifeScience
Cat# K54418M
discontinued |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(liquid, purified, filtered, PBS, BSA, azide) |
IH(P); assay |
CD105/ Endoglin |
|
CD105/ Endoglin
(PN-E2)
IgG1
|
Biodesign Meridian LifeScience
Cat# P61016M
discontinued |
|
monoclonal mouse
(liquid, PBS, BSA, azide) |
ELISA, IC, IH(F); not IH(P) |
in vitro cultured endothelial cells |
human |
CD105/ Endoglin
(8E11)
IgM
|
Interchim
Cat# O02570
produced by
Biodesign |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(liquid, purified, filtered, PBS, BSA, azide) |
IH(P); assay |
CD105/ Endoglin |
|
CD105/ Endoglin
(PN-E2)
IgG1
|
Interchim
Cat# O18830
produced by
Biodesign |
|
monoclonal mouse
(liquid, PBS, BSA, azide) |
ELISA, IC, IH(F); not IH(P) |
in vitro cultured endothelial cells |
human |
CD105/ Endoglin/ TGFβ1/3 R
(SN6h)
IgG1κ
|
Biotrend
Cat# Mob364
also distributed by ANAWA |
|
monoclonal mouse
(purified Ig fract) |
IH(P) |
CD105 |
|
CD105/ Endoglin/ TGF-βIII R
(SN6)
IgG1
|
Biodesign Meridian LifeScience
Cat# P33105M |
immunogen = membrane preparation of human non-T/non-B-cell-type acute lymphoblastic leukemia (ALL) |
monoclonal mouse
(liquid, purified, PBS, azide) |
FC, IH(F), IP |
endoglin /TGF-beta receptor |
human |
CD105/ Endoglin/ TGF-βIII R
(1G2)
IgG3
|
Interchim
Cat# O20210
produced by
Biodesign |
immunogen = CML blast crisis cells |
monoclonal mouse
(purified, filtered, PBS, BSA, lyophilized) |
FC |
stains endothelial cells from various tissues and syncytio trophoblasts. stains intensely on high endothelial venules (HEV) |
|
CD105/ TGFβR/ Endoglin
(8E11)
IgM κ
|
Antigenex
Cat#
M700720 |
|
monoclonal mouse
(liquid, purified, PBS, gelatin, azide) |
IH, IP, WB |
both isoforms (L and S) of CD105/Endoglin; will not block binding of TGF-β |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
CD105/ TGFβR/ Endoglin
(8E11)
IgM κ
|
Antigenex
Cat#
M700730, M700735 |
|
monoclonal mouse
(liquid, purified, PBS, gelatin, azide, FITC) |
FC, IH |
both isoforms (L and S) of CD105/Endoglin; will not block binding of TGF-β; lyses CD105+ cells in presence of complement |
human |
CD105/ TGFβR/ Endoglin
(SN6h)
IgG1κ
|
Lab Vision Thermo Scientific
Cat# MS-1290 |
immunogen = purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells |
monoclonal mouse
(liquid, protein G purified, PBS, with or without BSA and azide, or predilute ready-to-use) |
FC, IH(P), WB |
CD105/Endoglin, cell membrane |
human
(others not tested) |
CD105/ TGFβR/ Endoglin
(4G11)
IgG2a
|
Novocastra
Cat# NCL-CD105 |
immunogen = prokaryotic recomb. protein corresp. to a region of the external domain of the CD105 glycoprotein |
monoclonal mouse
(lyophilized, supernatant, azide) |
IH(P); not WB |
CD105/Endoglin/TGFβR, cell membrane |
human
(others not tested) |
CD105/ TGFβR/ Endoglin
(8E11)
IgM
|
Novocastra
Cat# NCL-ENDOGLIN |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(lyophilized, supernatant, azide) |
IH(F); not WB, IH(P) |
CD105/Endoglin/TGFβR, cell membrane |
human
(others not tested) |
CD105/
TGFβ R
(8E11)
IgM
|
Chemicon and Upstate are Millipore
Cat# CBL418 |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(purified, gel filtered, PBS, BSA, azide) |
FC, IH(P) |
CD105/ Endoglin/ TGFβ Receptor |
human
(others not tested) |
CD105/
TGFβ R
(8E11)
IgM
|
Chemicon and Upstate are Millipore
Cat# CBL418F |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(purified, gel filtered, PBS, BSA, azide, FITC) |
FC |
CD105/ Endoglin/ TGFβ Receptor |
human
(others not tested) |
CD105/
TGFβ R
(MJ7/18)
IgM
|
Chemicon and Upstate are Millipore
Cat# CBL1358 |
immunogen = inflamed mouse skin |
monoclonal rat
(purified, BBS, no preservcative) |
FC, IH, IP, WB;
not IH |
CD105/ Endoglin/ TGFβ Receptor |
mouse |
CD105/Endoglin/
TGFβ1/3 R
(poly)
|
Lab Vision Thermo Scientific
Cat# RB-9291 |
immunogen = recomb. protein encoding aa 26-176 of human CD105 |
polyclonal rabbit
(serum affinity purified, PBS, BSA and azide, or predilute ready to use) |
IH(P) |
CD105, 95kDa (monomer) and 190kDa (dimer), membrane and cytoplasmic |
human
(others not tested) |
DENTT
(poly)
IgG
|
US Biological
Cat #
D3155 |
immunogen = human Differentially Expressed Nucleolar TGFβ1 Target (DENTT) protein |
polyclonal rabbit
(liquid, affinity purified, PBS, azide, glycerol) |
WB |
DENTT protein |
human |
Endoglin/ CD105
(266)
IgG1κ
|
RDI /Fitzgerald
Cat# CD105-266 |
|
monoclonal mouse
(supernat, protein-G purified, PBS, azide) |
IF, IH(P) |
homodimeric integral membrane protein comprised of 95Kd subunits (CD105) |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
Endoglin/ CD105
(8E11)
IgM
|
RDI /Fitzgerald
Cat# CBL418/ CBL418-FT |
immunogen = PMA-induced U-937 cells |
monoclonal mouse
(purified, gel filtered, PBS, BSA, azide) |
IH(P); Differ |
Endoglin/ CD105 |
human |
Endoglin/ CD105
(SN6/ N1-3A1)
IgG1κ
|
RDI /Fitzgerald
Cat# CD105-SN |
immunogen = membrane preparation of human B-lineage leukemia cells |
monoclonal mouse
(purified, also available as PE, FITC, Biotin conj.) |
FC, IP; (F) |
recognizes a CD105 homodimer, wt. 160kd; in purfied form will augment the binding of TGFβ1 to CD105 expressing leukemia cells. |
human |
Endoglin/ TGFβ1R
(P3D1)
IgG2a
|
Covance Research Products, Inc.
Cat# MMS-478R |
immunogen = IL-1β activated human umbilical vein endothelial cells (epitope within the N-terminal 204 aa, part of extracellular domain). |
monoclonal mouse
(raw ascites fluid) |
IF, IP, WB |
endoglin protein on the surface of resting or activated human endothelial cells |
|
Endoglin/
TGFβ-RIII
(MJ7/18)
IgG2a
|
Santa Cruz
Cat #
SC-18893 |
immunogen = inflamed mouse skin tissue |
monoclonal mouse
(IgG, PBS, azide, gelatin) |
FC, IH |
TGFβ-RIII |
mouse |
LAP TGF-β1
(27232)
IgG1
|
R&D Systems
Cat #
MAB-2463 |
immunogen = purified insect cell line Sf 21-derived, rhLAP (rhTGF-β1) |
monoclonal mouse
(ascites, protein-G purified, filtered, PBS, lyophilized) |
ELISA, FC |
LAP (TGF-β1) |
human, mouse |
LAP TGF-β1
(27232)
IgG1
|
R&D Systems
Cat #
FAB-2463P |
|
monoclonal mouse
(PBS, azide, conj. PE) |
FC |
LAP (TGF-β1) |
human |
LAP TGF-β1
(27235)
IgG1
|
R&D Systems
Cat #
MAB-246 |
immunogen = purified insect cell line Sf 21-derived, rhLAP (rhTGF-β1) |
monoclonal mouse
(protein-G purified, filtered, PBS, trehalose, lyophilized) |
ELISA, Neut, WB |
recombinant as well as natural human TGF-β1 LAP (latency associated peptide) |
human |
LAP TGF-β1
(27240)
IgG2a
|
R&D Systems
Cat #
BAM-2462 |
immunogen = insect cell line Sf21-derived recomb. human TGF-β1 latency associated peptide |
monoclonal mouse
(ascites, affinity purified, filtered, Biotin, PBS, lyophilized) |
ELISA |
human LAP (TGF-β1) and human latent TGF-β1 |
human |
LAP TGF-β1
(9005)
IgG1
|
R&D Systems
Cat #
MAB-2461 |
immunogen = purified CHO cell-derived recombinant human Latent TGF-β1 |
monoclonal mouse
(ascites, protein-A purified, filtered, PBS, lyophilized) |
ELISA |
rhLAP (TGF-β1); not LAP from the TGF-β2 precursor |
human |
LAP TGF-β1
(poly)
IgG
|
R&D Systems
Cat #
BAF-246 |
immunogen = purified insect cell line Sf 21-derived recomb. human latency associated peptide |
polyclonal goat
(affinity purified, filtered, Biotin, PBS, lyophilized) |
WB |
rhLAP; not mature rhTGF-β1, pTGF-β1.2, pTGF-β2, rhTGF-β3 and raTGF-β5 |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
LAP TGF-β1
(poly)
IgG
|
R&D Systems
Cat #
AF-246-NA |
immunogen = purified CHO cell-derived recombinant human TGF-β1 Lateny Associated Peptide (LAP) |
polyclonal goat
(affinity purified, filtered, PBS, trehalose, lyophilized) |
ELISA, IH, Neut, WB |
TGF-β1 LAP |
human |
LAP TGF-β5
(poly)
IgG
|
R&D Systems
Cat #
AB-246-NA |
immunogen = CHO cell-derived recomb. human TGF-β1 latency associated peptide |
polyclonal goat
(protein-G purified, filtered, PBS, lyophilized) |
ELISA, IH(P), Neut, WB |
LAP derived from the TGF-β1 precursor; not with LAP from the TGF-β2 precursor |
human |
Latent TGF-β1
(kit)
|
R&D Systems
Cat# EL-246 (1 kit)
Cat# PEL-246 (1 pack)
Cat# XEL-246 (6 pack) |
|
(immunospot assay, detection antibody -biotin, streptavidin AP, latent TGF-β1 positive control) |
ELISpot |
recombinant as well as natural human Latent Associated Peptide (TGF-β1) |
human |
Latent TGF-β1
(kit)
|
R&D Systems
Cat# SEL-246 |
|
(capture antibody, detection antibody, chromogen) |
ELISpot |
both recombinant as well as natural human TGF-β1 Latent Associated Peptide (LAP) |
human |
Latent TGF-βbp2
(327318)
IgG2b
|
R&D Systems
Cat# MAB3850 |
immunogen = purified, NS0-derived, recombinant human Latent Transforming Growth factor beta binding protein 2 (rhLTBP-2; aa36-1821; Accession #NP_000419) |
monoclonal mouse
(liquid, filtered, PBS, trehalose) |
ELISA, WB |
LTBP-2; no cross-reactivity with rmLTMP-4 |
human |
Latent TGF-βbp4
(poly)
IgG
|
R&D Systems
Cat# AF2885 |
immunogen = purified, NS0-derived, recombinant mouse Latent Transforming Growth factor beta binding protein 4 isoform 1(rhLTBP-4; aa25-1666; Accession #Q8K4G1; with R542-P547 deletion and D625G substitution) |
polyclonal goat
(liquid, filtered, PBS, trehalose) |
ELISA, WB |
LTBP4; less than 1% cross-reactivity with rmLTMP-1 and rhLTMP-2 |
human |
LTBP-1
(35409)
IgG1
|
R&D Systems
Cat #
MAB-388 |
immunogen = partially purified large latent TGFβ1 complex from human platelets |
monoclonal mouse
(ascites, protein-A purified, filtered, PBS, lyophilized) |
ISP, WB |
latent TGF-β1binding protein (LTBP-1) from the large latent TGF-β1 complex |
human |
Pan TGFβ
(poly)
IgG
|
Sigma Aldrich
Cat# T4929 |
immunogen = mix of rhTGF-β 1, pTGF-β1.2, pTGF-β2, and rampTGF-β5 |
polyclonal rabbit
(protein-A purified, filtered, PBS, no preserves, lyophilized) |
ELISA, IB, Neut |
will neutralize hTGF-β1,pTGF-β1.2, pTGF-β2,hTGF-β3, and rampTGF-β5; no cross with other cytokines |
human, porcine, amphibian (various) |
Pan TGF-β
(poly)
IgG
|
R&D Systems
Cat #
AB-100-NA |
immunogen = mix of purified rhTGF-β1, porcine TGF-β1.2, porcine TGF-β2 and recomb. amphibian TGF-β5 |
polyclonal rabbit
(protein-G purified, PBS, trehalose, lyophilized) |
ELISA, IH(P), Neut, WB |
TGF-β1, pTGF-β1.2, pTGF-β2, TGF-β3 and raTGF-β5 |
human, porcine, amphibian (various), chicken |
pro-TGFα
(poly)
|
Cell Signaling
Cat# 3715 |
immunogen = synthetic peptide, KLH coupled, corr. to the N-terminal seq. of TGF-β |
polyclonal rabbit
(protein A, affinity purified, HEPES, BSA, glycerol) |
WB |
endogenous TGFα precursor protein but not mature; no cross reaction with other family members. |
human, mouse
(expect rat) |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
PTGFβ
(poly)
IgG
|
Autogen Bioclear
Cat# SC-10603
produced by Santa Cruz |
immunogen = within an internal region of human PTGF-β |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IH, WB |
Placental TGFβ |
human |
PTGFβ
(poly)
IgG
|
Autogen Bioclear
Cat# SC-10606
produced by Santa Cruz |
immunogen = within an internal region of rat PTGF-β |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IH, WB |
Placental TGFβ |
rat, mouse |
PTGFβ
(poly)
IgG
|
Autogen Bioclear
Cat# SC-10607
produced by Santa Cruz |
immunogen = rat PTGF-β1, near the amino terminus |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IH(P), WB |
Placental TGFβ |
rat, mouse |
PTGFβ
(poly)
IgG
|
Santa Cruz
Cat #
SC-10603 |
immunogen = within an internal region of human PTGF-β |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
IF, WB |
Placental TGFβ |
human |
PTGFβ
(poly)
IgG
|
Santa Cruz
Cat #
SC-10606 |
immunogen = within an internal region of rat PTGF-β |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
IH, WB |
Placental TGFβ |
rat, mouse |
PTGFβ
(poly)
IgG
|
Santa Cruz
Cat #
SC-10607 |
immunogen = rat PTGF-β1, near the amino terminus |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
IH, WB |
Placental TGFβ; not the mature strain |
rat, mouse |
PTGFβ
(poly)
IgG
|
US Biological
Cat #
N0017 |
immunogen = ovalbumin conj. synthetic peptide corr. to aa287-302 of human NAG1/PTGF-β |
polyclonal rabbit
(liquid, affinity purified, Tris, azide, glycerol) |
IH(P), WB |
pro- and secreted- forms of NAG-1/PTGF-β,~35kD and 10-15kD respectively |
human
(not mouse) |
TAK1
(poly)
|
Sigma Aldrich
Cat# T9689 |
immunogen = synthetic peptide corr. to aa 561-579 of mouse TGF-β activating kinase (TAK1), conj. to KLH. |
polyclonal rabbit
(liquid, affinity purified, isolated, Tris, glycerol) |
IB, IP |
TAK1 (TGF-β Activating Kinase) 70 kDa |
human, rat, mouse |
TAK1
(poly)
IgG
|
US Biological
Cat# T0725-10 |
immunogen = synthetic peptide corr. to aa 561-579 of mouse TGF-β activating kinase (TAK1), conj. to KLH. |
polyclonal rabbit
(liquid, affinity purified, Tris- glycine saline, glycerol, azide) |
IB, IP |
TAK1 (TGF-β Activating Kinase) 70 kDa |
human, mouse, xenopus |
TAK1
(poly)
IgG
|
US Biological
Cat# T0725-15A |
immunogen = synthetic peptide of human TGF-β activating kinase (TAK1) |
polyclonal rabbit
(liquid, purified, PBS, azide) |
WB |
TAK1 (TGF-β Activating Kinase) 70 kDa |
human |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
TAK1 binding protein 2
(poly)
IgG
|
US Biological
Cat# T0725-25 |
immunogen = recombinant protein fragment corresp. to the last 186 aa from the human TAB 2 |
polyclonal rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IF, WB |
TAB 2 (TGF-β Activated Kinase 1 Binding Protein 2) |
human, mouse |
TGFα
(mono)
IgG1
|
Autogen Bioclear
Cat# SC-36
produced by Santa Cruz |
epitope = corresponding to a domain encompassing cysteine 16 of human TGFα |
monoclonal mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
precursor and mature forms of TGFα |
human, rat, mouse |
TGFα
(mono)
IgG1
|
Autogen Bioclear
Cat# SC-57444
produced by Santa Cruz |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, azide, supernatant) |
ELISA |
TGFα |
human, mouse |
TGFα
(mono)
IgG2a
|
Autogen Bioclear
Cat# SC-57445
produced by Santa Cruz |
|
monoclonal mouse
(liquid, azide, affinity purified) |
IF, IH(F, P) |
TGFα |
human, rat, mouse |
TGFα
(mono)
IgG1
|
Autogen Bioclear
Cat# SC-57446
produced by Santa Cruz |
immunogen = human TGFα |
monoclonal mouse
(liquid, azide, supernatant) |
IF, IH(F, P), WB |
TGFα; no cross reaction with EGF or TGFβ |
human |
TGFα
(mono)
IgG1
|
Autogen Bioclear
Cat# SC-57447
produced by Santa Cruz |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, azide, PBS, gelatin) |
IH(F, P), WB |
TGFα |
human |
TGFα
(mono)
IgG1
|
Autogen Bioclear
Cat# SC-73148
produced by Santa Cruz |
|
monoclonal mouse
(liquid, supernatant) |
|
TGFα |
human, mouse |
TGFα
(poly)
IgG
|
Autogen Bioclear
Cat# SC-1338
Cat# SC-1338 P (peptide)
produced by Santa Cruz |
immunogen = peptide mapping at C-terminus of human TGFα |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IF, IH(F), IP, Neut, WB |
TGFα, precursor and mature forms as well as BTC |
human, rat, mouse |
TGFα
(poly)
IgG
|
Autogen Bioclear
Cat# SC-27155
Cat# SC-27155 P (peptide)
produced by Santa Cruz |
immunogen = peptide mapping near the N-terminus of human TGFα |
polyclonal goat
(liquid, affinity purified, PBS, azide, gelatin) |
IF, IH(F), WB |
TGFα, precursor and mature forms |
human, rat, mouse |
TGFα
(poly)
IgG
|
Autogen Bioclear
Cat# SC-9043
produced by Santa Cruz |
immunogen = aa 40-89 of human TGFα |
polyclonal goat
(liquid, PBS, azide, gelatin) |
IF, IH(F), IP, WB |
TGFα, precursor and mature forms |
human, rat, mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
TGFα
(1E8-269)
IgG1
|
Biogenesis AdB Serotec
Cat# MCA1384S |
immunogen = 32-40 aa seq. linked to thyroglobulin |
monoclonal mouse
(liquid, supernatant, tris/HCl, FCS, BSA, azide) |
ELISA |
TGF-α |
human, mouse |
TGFα
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# AHP284B |
immunogen = highly purified recombinant TGFα |
polyclonal rabbit
(lyophilized, affinity purified, biotinylated) |
ELISA, WB |
TGF-α |
human |
TGFα
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# 9129-8650 |
immunogen = native TGFα |
polyclonal sheep
(liquid, purified, PBS, azide) |
ELISA, IH(P) |
TGF-α |
rat |
TGFα
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# 9129-7995 |
immunogen = mature human recombinant TGFα conj. to KLH |
polyclonal sheep
(liquid, ion exchange, BBS, azide) |
ELISA |
TGF-α; no reaction with EGF or TGF-α |
human, rat, bovine |
TGFα
(poly)
IgG
|
Biogenesis AdB Serotec
Cat# AHP284G |
immunogen = highly purified recombinant TGFα |
polyclonal rabbit
(liquid, PBS, affinity purified) |
ELISA, FA, WB |
TGF-α |
human |
TGFα
(1E8-G6)
IgG/IgM
|
Biotrend
Cat# BT91-2979-04 |
immunogen = TGFα peptide-HMW carrier |
monoclonal mouse
(liquid, PBS, azide, ammonium sulfate fract.) |
ELISA, IF, IH(P) |
TGFα |
|
TGFα
(MF9/TG86)
IgG1/IgM
|
Biotrend
Cat# Mob352 |
immunogen = synthetic peptides from rat (MF9) and human (TG86) TGFα |
monoclonal mouse
(purified Ig fract, azide) |
IH(P) |
TGFα |
human, rat |
TGFα
(poly)
IgG
|
Biotrend
Cat# BT91-2986-50 |
immunogen = native TGFα |
polyclonal sheep
(liquid, purified PBS, azide) |
ELISA, IH(P) |
TGFα |
rat |
TGFα
(poly)
IgG
|
Biotrend
Cat# BT91-2980-24 |
immunogen = recombinant mature human TGFα |
polyclonal sheep
(liquid, serum, azide) |
IH(P), IP |
TGFα; not EGF or TGFβ |
human, rat, bovine |
TGFα
(poly)
IgG
|
Biotrend
Cat# BT91-2979-95 |
immunogen = recombinant mature human TGFα, conj. KLH |
polyclonal sheep
(liquid, BBS, azide, ion exchange chromatography) |
ELISA |
TGFα; not EGF or TGFβ |
human, rat, bovine |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
TGFα
(mono)
IgG1
|
Santa Cruz
Cat #
SC-36 |
epitope = domain encompassing cysteine 16 of human TGFα |
monoclonal mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
precursor and mature TGFα |
human, rat, mouse |
TGFα
(mono)
IgG1
|
Santa Cruz
Cat #
SC-57444 |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, azide, supernatant) |
IF, IP, WB |
TGFα |
human, mouse |
TGFα
(mono)
IgG1
|
Santa Cruz
Cat #
SC-73148 |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, azide, supernatant) |
ELISA |
TGFα |
human, mouse |
TGFα
(mono)
IgG1
|
Santa Cruz
Cat #
SC-57446 |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, azide, supernatant) |
IF, IH(P), WB |
TGFα; no cross with EGF or TGFβ |
human |
TGFα
(mono)
IgG1
|
Santa Cruz
Cat #
SC-57447 |
immunogen = aa 32-40 of human TGFα |
monoclonal mouse
(liquid, PBS, azide, gelatin) |
IF, IH(P), WB |
TGFα |
human |
TGFα
(poly)
IgG
|
Santa Cruz
Cat# SC-1338
Cat# SC-1338P (blocking peptide) |
epitope = mapping at the C-terminus of human TGFα |
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
precursor and mature TGFα and BTC |
human, rat, mouse, pig |
TGFα
(poly)
IgG
|
Santa Cruz
Cat# SC-9043 |
immunogen = aa 40-89 representing mature human TGFα |
polyclonal rabbit
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
precursor and mature TGFα and BTC |
human, rat, mouse |
TGFα
(poly)
IgG
|
Santa Cruz
Cat# SC-27155
Cat# SC-27155P (blocking peptide) |
epitope = mapping at the N-terminus of human TGFα |
polyclonal goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, WB |
precursor and mature TGFα |
human, rat, mouse |
TGFα
(1E8-269)
IgG1
|
AbD Serotec
Cat# MCA1384S |
immunogen = 32-40 aa seq. linked to thyroglobulin |
monoclonal mouse
(liquid, supernatant, tris/HCl, FCS, BSA, azide) |
ELISA |
TGF-α |
human, mouse |
TGFα
(poly)
IgG1
|
AbD Serotec
Cat # AHP284B
|
immunogen = recombinant human TGF-α |
polyclonal mouse
(liquid, PBS, no preservative, protein G purified, biotinylated conj.) |
ELISA, WB |
TGF-α |
mouse |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
TGFα
(poly)
IgG
|
AbD Serotec
Cat# 9129-8650 |
immunogen = native TGFα |
polyclonal sheep
(liquid, purified, PBS, azide) |
ELISA, IH(P) |
TGF-α |
rat |
TGFα
(poly)
IgG
|
AbD Serotec
Cat# 9129-7995 |
immunogen = mature human recombinant TGFα conj. to KLH |
polyclonal sheep
(liquid, ion exchange, BBS, azide) |
ELISA |
TGF-α; no reaction with EGF or TGF-α |
human, rat, bovine |
TGFα
(poly)
IgG
|
AbD Serotec
Cat# AHP284G |
immunogen = highly purified recombinant TGFα |
polyclonal rabbit
(liquid, PBS, affinity purified) |
ELISA, FA, WB |
TGF-α |
human |
TGFα
(0.N.588)
IgG1, κ
|
US Biological
Cat #
T8250-03 |
immunogen = 17-aa synthetic peptide from the C-terminus of rat TGFα |
monoclonal mouse
(liquid, protein G purified, PBS, BSA, azide) |
WB |
TGFα |
human, rat, mouse, bovine |
TGFα
(0.N.589)
IgM, κ
|
US Biological
Cat #
T8250-04 |
immunogen = 10-aa synthetic peptide (aa34-43) of human TGFα |
monoclonal mouse
(liquid, PBS, BSA, azide, purified; available without BSA/azide) |
ELISA, IH(P) |
TGFα |
human |
TGFα
(3F379/3F379H)
IgM, κ and IgG1, κ
|
US Biological
Cat# T8250-15
Cat# T8250-15X (without BSA/azide) |
immunogen = synthetic peptide from rat and human TGFα |
monoclonal mouse
(liquid, PBS, BSA, azide, purified ascites; available without BSA/azide) |
IH(P) |
TGFα |
human, rat |
TGFα
(3H3079)
|
US Biological
Cat #
T8250-03A |
immunogen = aa32-40 linked to thyroglobulin |
monoclonal mouse
(liquid, supernatant Tris-HCl, FCS, azide) |
ELISA |
TGFα |
human, mouse |
TGFα
(8A261)
IgG1
|
US Biological
Cat #
T8250-02 |
immunogen = recomb. human TGFα |
monoclonal mouse
(ion exchange chromatography, PBS, lyophilized) |
ELISA |
TGFα |
human |
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-03C |
immunogen = recombinant human TGFα |
polyclonal rabbit
(lyophilized, PBS, affinity purified) |
ELISA, Neut, WB |
TGFα |
human |
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-06 |
immunogen = synthetic analog of the C terminal of rat transforming growth factor alpha (rTGFα) covalently attached onto a carrier protein |
polyclonal rabbit
(lyophilized, serum) |
ELISA, IC, WB |
TGFα and hEGF; no reaction to mEGF, hAtrial Natriuretic Peptide, hACTH (1-39), hCalcitonin, bPTH (1-84), Somatostatin and Vasoactive Intestinal Peptide |
human, rat |
Antigen
(Clone)
IgG |
Distributor
(Producer) |
Immunogen
(Epitope) |
Host
(Formulation) |
Methods
glossary |
Specificity |
Reactivity top |
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-06D |
immunogen = synthetic analog of the C-terminal of transforming growth factor alpha (TGFα) covalently attached onto a carrier protein |
polyclonal rabbit
(lyophilized, serum) |
ELISA, IC, WB |
TGFα; no reaction to hEGF |
|
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-06F |
immunogen = peptide analog of the C-terminal of transforming growth factor alpha (TGFα) covalently attached onto a carrier protein |
polyclonal rabbit
(lyophilized, serum) |
ELISA, IP, RIA |
TGFα; no reaction to hEGF, rAtrial Natriuretic Factor, Insulin-like Growth Factor-1, Insulin-like Growth Factor-2 and Calcitonin (Human) |
rat |
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-06H |
immunogen = peptide analog of the C-terminal of transforming growth factor α (TGFα) covalently attached onto a carrier protein |
polyclonal rabbit
(lyophilized, serum) |
ELISA, IC, WB |
TGFα and hEGF; no reaction to mEGF, hAtrial Natriuretic Factor, hACTH (1-39), hCalcitonin, bPTH (1-84), Somatostatin and Vasoactive Intestinal Peptide |
human, rat |
TGFα
(poly)
IgG
|
US Biological
Cat #
T8250-06K |
immunogen = peptide analog of the C-terminal of transforming growth factor α (TGFα) covalently attached onto a carrier protein |
polyclonal rabbit
(lyophilized, serum) |
ELISA, IC, WB |
TGFα and hEGF; no reaction to mEGF, hAtrial Natriuretic Factor, hACTH (1-39), hCalcitonin, bPTH (1-84), Somatostatin and Vasoactive Intestinal Peptide |
human, rat |
TGF-α
(134A-2B3)
IgG1,κ
|
Abcam
Cat# ab16858 |
immunogen = recombinant full length protein (human)
|
monoclonal mouse
(liquid, gelatin, NaPO4, azide) |
WB |
TGF-α |
human, rat
(others not tested) |
TGF-α
(189-2130.1)
IgG1;
|
Abcam
Cat# ab16811 |
immunogen = recombinant mature 6kDa protein (human)
|
monoclonal mouse
(lyophilized, PBS, BSAm purified) |
Neut |
TGF-α |
human, rat
(others not tested) |
TGF-α
(1E8-269)
IgG1;
|
Abcam
Cat# ab11456 |
immunogen =
aa 32-40 seq. linked to thyroglobulin
|
monoclonal mouse
(liquid, supernatant, tris-HCl, FCS, azide) |
ELISA |
TGF-α |
human, mouse
(others not tested) |
TGF-α
(213-4.4)
IgG2a,κ
|
Abcam
Cat# ab16768 |
immunogen = recombinant full length protein (human)
|
monoclonal mouse
(liquid, gelatin, NaPO4, gelatin, azide) |
IH(F, P) |
TGF-α (both native and some denatured forms); no cross-reaction with human or mouse EGF |
human, rat, mouse, hamster, dog
(others not tested) |
TGF-α
(MF9 + TG86)
|
Abcam
Cat# ab2385 |
immunogen =
synthetic peptides from rat (MF9) and human (TG86) TGF-α
|
monoclonal mouse
(liquid, PBS, azide, protein A purified) |
IH(F, P), WB |
reacts with a 6kDa protein known as TGF-α |
human
(others not tested) |
TGF-α
(P/T1)
IgG1,κ
|
Abcam
Cat# ab9578 |
immunogen = synthetic peptide fragment (32-40 aa seq. of 50 aa) of transforming growth factor alpha (TGF-alpha)
|
monoclonal mouse
(liquid, PBS, BSA, azide) |
ELISA, IC, IH(F, P), WB |
TGF-α |
human
(others not tested) |
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