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Home: Research: Compendia: Antibodies

Antibody Directory


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Antibodies to Amyloid Precursor & Amyloid Precursor-related Proteins
From the Laboratories
Antigen (Clone) IgG	Name (Source) used in	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
alpha APP CTFs (poly)	CT15 (S Soriano, 2001 Paper, JBC online, Dr. Edward Koo, University of California, San Diego), References: Lee, S, 2010 Banwait, 2008 Rockenstein, 2007 Galvan, 2006	immunogen = synthetic peptide corresp. to the C-terminal 15 residues of APP (aa 680-695)	polyclonal rabbit	IP, WB	APP C-terminal fragments; recognizes the last 15 COOH-terminal amino acid residues of APP₆₉₅	human, mouse
APP (1G7)	(Dr. Edward Koo, University of California, San Diego) Paper	immunogen = human APPs purified from APP-transfected CHO cells (epitope between aa 380 and 365, in the extracellular region)	monoclonal mouse	IF, IP	recognizes an APP surface domain, excluding both the KPI and Aβ domains	human, mouse
APP (22C11)	(Konrad Beyreuther, Center of Molecular Biology, University of Heidelberg, Heidelberg, Germany) Paper Reference: Kuhn, 2010	immunogen = a bacterial APP fusion protein (Fd-APP 695), (epitope in ectodomain, at residues 66-81 of APP)	monoclonal mouse	IH, WB	detects a variety of mature as well as immature APP species which differ in their degree of glycosylation	monkey, human, rat, mouse
APP (4B4) IgG_2a	(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany) Paper Reference: Kuhn, 2010	immunogen = a peptide comprising amino acids 11–16 of the Aβ sequence (EVHHQK-COOH)	monoclonal rat	WB	specifically detects APPsα ending in amino acids 15 and 16 (APPs-15 and APPs-16), but does not detect shorter APPs species, including APPsβ' and APPsβ; does not detect full-length APP	human (not mouse)
APP (5A3)	(Dr. Edward Koo, University of California, San Diego) Paper	immunogen = C-terminally truncated secreted βPP derivatives purified from conditioned medium of βPP-transfected CHO cells in Freund’s adjuvant	monoclonal mouse (affinity purified)	IP	recognizes the mid-region of the pAPP ectodomain	human
APP (7A6) IgG_2a	(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany) Paper Reference: Kuhn, 2010	immunogen = a peptide comprising amino acids 10–15 of the Aβ sequence (YEVHHQ-COOH)	monoclonal	WB	specifically detects APPsα; detects also murine APPsα, but not APPsβ and APPsβ'	human, mouse
APP (8E5)	anti-APP 8E5 (P. Seubert and D. Schenk, Elan Pharmaceuticals, South San Francisco, CA) References: Britschgi, 2009 Rose, 2009 Maier, 2008 Roberson, 2007 Esler, 2002, Van Uden, 2002 Choi, 2006	immunogen = residues 444-592 of APP	monoclonal mouse	WB	residues 444-592 of APP in the AβPP695 ectodomain and AβPPs	human (reference: McConlogue L, Castellano F, deWit C, Schenk D, and Maltese W.A. J. Biol. Chem. 271, 1343-1348, 1996)
APP (BAWT) IgG_2a	(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany) Paper, Ulrich et al. Reference: Kuhn, 2010	immunogen = the peptide ISEVKM derived from the amino acids directly preceding the APPβ-secretase cleavage site	monoclonal rat	IP, WB	detects APPsβ at the β cleavage site	human
APP (poly)	anti-APP 369 (Sam Gandy, Mount Sinai Alzheimer's Disease Research Center, NYC) References: Lane RF, 2010 Vieira SI, 2010 Pavlov PF, 2010 Li H, 2010 Gandy, 2010 Zhang, 2009 Liu, Y, 2009 Hoe, 2009 Tampellini, 2007 White, 2006 Puglielli, 2001 Li, 2003	immunogen = entire cytoplasmic tail of APP (paper)	polyclonal rabbit	IH, IP	C-terminal APP	human, mouse
APP (poly)	APP B5 (5313) (Christian Haass, Ludwig-Maximilians-Universität München, Munich, Germany Paper, Steiner et al. References: Kuhn, 2010 Stamer, 2002	immunogen = a sequence in the ectodomain, aa 444-592, of human APP	polyclonal	IP	reactive with APPs, ectodomain	human
Antigen (Clone) IgG	Name (Source) used in	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
APP (poly)	anti-APP C7 (Dr. Dennis Selkoe, Harvard Univ. Medical School, Boston, MA) Reference: Puglielli, 2001, by Yu, 2001	last 20 residues of APP	polyclonal rabbit	IH		(reference: Knops, 1995)
APP (poly)	anti-APP 'Saeko' (Prof. Mikio Shoji, Gunma University School of Medicine, Gunma, Japan) Reference: Yu, 2001	C-terminus of APP	polyclonal	IH		(reference: Kawarabayashi T, Shoji M., et al., Neurobiol Aging 17:215-222, l996)
APP (poly)	anti-APP (U995) (Thomas Sudhof, The Center for Basic Neuroscience, Department of Molecular Genetics, The University of Texas Southwestern Medical Center) Reference: Cao, 2001	C-terminus of APP (U995)	polyclonal	WB		(reference: Cao X, Sudhof T. A trans criptionally [correction of transcriptively] active complex of APP with Fe65 and histone acetyl transferase Tip60.Science. 2001 Jul 6; 2935527)
APP (poly)	anti-APP C9 (Dr. Dennis Selkoe, Harvard Univ. Medical School, Boston, MA) References: Muresan, 2009 Sato, 2007	synthetic peptide corresp. to APP_732-751, (APP₇₅₁ numbering)	polyclonal rabbit	IP, WB		(Paper: Kimberly et al., 2005)
APP (poly)	anti-APP α-1 (Dr. Dennis Selkoe, Harvard Univ. Medical School, Boston, MA	synthetic peptide of residues 649-664	polyclonal rabbit	IH, WB	APP	human
APP (poly)	R1736 (Dr. Dennis Selkoe, Harvard Univ. Medical School, Boston, MA) Reference: Maier, 2008,	immunogen = synthetic peptide of amino acids 595–611 of APP	polyclonal rabbit (antiserum)	IP, WB	APP epitope corresponds to positions -2 to +15 of human Abeta, and it presents 90% (15 out of 17 amino acids) of sequence homology with the hamster sequence which is identical to other rodents such as mouse	human, rodent (various)
APP βCTF and Aβ1-X (N25)	JRF/AβN/25 (Paul Mathews, New York University, School of Medicine, Center for Dementia Research) Paper References: Jiang Y., 2010	epitope at first 7 residues of Aβ; requires β-cleavage	monoclonal mouse	ELISA, IF, WB	recognizes βCTF and Aβ1-X	human, mouse
APP 591-596 (poly)	192 S. Sinka (Elan Pharma ceuticals) Reference: Kojro, 2006		polyclonal rabbit
APP C terminal (poly)	6687 Christian Haass, Ludwig-Maximilians-Universität München, Munich, Germany Paper, Steiner et al. Reference: Kojro, 2006	immunogen = the 20 most C-terminal amino acids of human APP	polyclonal		human APP C-terminal	human
APP cleaved at Asp664 (poly)	APPNeo (Dr. Dale Bredesen, Buck Institute for Age Research) Paper, Paper Reference: Banwait, 2008	immunogen = the peptide ₆₅₇CIHHGVVEVD₆₆₄, which includes the nine amino acids immediately preceding the caspase cleavage site at position 664 in AβPP, coupled to KLH	polyclonal rabbit (affinity purified, borate buffer)	ELISA, IP, WB	recognizes the neo-epitope generated by cleavage of AβPP at Asp664 in its intracellular, C-terminal domain; recognizes APP that was truncated at Asp664 but not intact APP	human, mouse
Antigen (Clone) IgG	Name (Source) used in	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
APP CTF (B83.1)	B83.1 (Dr. B. De Strooper, Katholieke Universiteit Leuven)		monoclonal mouse	IP, WB	recognizes C-terminal of APP and C-terminal fragments	human, mouse
APP, C terminal (poly)	APPCter-C17 A. Delacourte, N. Sergeant Paper Reference: Lesné, 2006	immunogen = synthetic peptide corresp. to the last 17 aa. of human APP	polyclonal rabbit (serum)	IP, WB	APP, C terminal	human
APP, C-terminal (poly)	Ab54 (SmithKline Beecham Pharmaceuticals, Harlow, UK) Reference: Parkin, 2007	C-terminus of cytosolic domain of APP	polyclonal	IB	recognizes the C-terminal cytosolic domain of APP; full length APP and CTFs	(reference: Parvathy S, Hussain I, et al., Biochemistry 1998, 37, 1680-1685 paper)
APP, C-terminal (poly)	APP 140 (Jochen Walter, Department of Neurology, University of Bonn, Bonn, Germany) Paper Reference: Heneka, 2010	immunogen =a peptide representing the last 15 C-terminal amino acids of human APP	polyclonal rabbit	IH, WB	detects APP	human, mouse
APP, C-terminal (poly)	anti-APP C15 (Dr. Kei Maruyama, Saitama Medical University, Saitama, Japan). Paper Reference: Kitazume, 2010	imunogen = residues 681-695 of APP	polyclonal rabbit	IC, IH, IP, WB	recognizes C-terminal region of APP	human
APP, CTFs (C1/6.1) IgG₁	C1/6.1 (Paul Mathews, New York University, School of Medicine, Center for Dementia Research) Research paper References: Pavlov PF, 2010 Jiang Y., 2010 Loane, 2009 Hoe, 2009	immunogen = synthetic peptide, first 20 amino acids of the C-terminal, cytoplasmic domain of APP (residues 676–695 of APP695)	monoclonal mouse (ascites)	IP, WB	recognizes full length APP and APP CTFs	human, mouse
APP, full length (poly)	anti-APP C8 (Dr. Dennis Selkoe, Harvard Univ. Medical School, Boston, MA) References: Davis, 2010 Senechal, 2008 Wang, 2007	residues 676-695, comprising the 20 C-terminal amino acids of human βAPP cytoplasmic domain. Paper, Selkoe et al.	polyclonal rabbit	DB, IC, IH, WB	recognizes full length APP and all CTFs	human, mammal (various)
APP, intracellular doman (poly)	36BO (X Tian et al., 2007 Paper, Subcell Biochem) Laboratory of Michael Przybylski See paper on Mass spectrometric approaches for elucidation of antigen /antibody recognition structures	immunogen = recomb. GST-C-APP fusion protein [724–770]. (epitope at aa. 727-737) Epitope determined by epitope excision /extraction -mass spectrometry. Similar differential epitope identification process identified the epitope of the Jonas-mAb as aa. 740-747, including the potential Caspase-3 cleavage site	polyclonal rabbit	IH, IP, WB	recognizes APP, C-terminal, intracellular doman. The polyclonal cAPP antibody 36BO can be used for detecting full length APP and large C-terminal APP fragments, while the monoclonal Jonas-mAb should be a useful tool for the study of APP processing in apoptotic neuronal cells and in brain extracts	human
APP/Aβ (poly)	APP 1736 (Christian Haass, Adolf Butenandt Institute, University of Munich) Reference: Yu, 2001	immunogen = N-terminus of Amyloidβ, aa 595-611 of APP	polyclonal	WB		(reference: Haass C, Schlossmacher M G, Hung AY, ... Teplow DB, Selkoe DJ. Nature 359, 322-325, 1992)
APPL (mono)	anti-APPL (Kalpana White, Department of Life Sciences, Brandeis Univ, Waltham MA) Reference: Finley, 2003		monoclonal (affinity purified)	IC, WB		(reference: Torroja L, Luo, L, White K., J Neurosci 16:4638-4650, 1996)
Antigen (Clone) IgG	Name (Source) used in	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
sAPPβ	Ab1A9 (SmithKline Beecham Pharmaceuticals, Harlow, UK) Reference: Parkin, 2007	C-terminus of large ectodomain of APP		IB	recognizes the neoepitope formed at the C-terminus of the large ectodomain of APP following β-secretase cleavage. As this neoepitope is cryptic in both full-length APP and sAPPα, Ab1A9 recognizes only sAPPβ	(reference: Parvathy S, Hussain I, et al., Biochemistry 1998, 37, 1680-1685 paper)
From the Manufacturers
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
β-APP (3G12) IgM	Histo-Line Cat# MONX10740	immunogen = prokaryotic rh fusion protein corresp. to the extracellular protein of APP between the Kunitz protease inhibitor domain and the beta amyloid region	monoclonal mouse (lyophilized, supernatant azide)	IH(P)	APP; no cross-reaction with APP-like proteins	human
β-APP (LN27) IgG₁κ	Histo-Line Cat# 13-0200	immunogen = rh β-APP695; epitope within the first 200 aa in the β-APP N-terminus	monoclonal mouse (liquid, ascites, Ig frac., PBS, azide)	ELISA, IH(F, P), IP, WB	β-APP 695, 751, 770	human
β-APP (poly)	Histo-Line Cat# 51-2700	immunogen = 22 aa synthetic peptide from the C-terminal of human β-APP	polyclonal rabbit (liquid, PBS, azide, affinity purified)	IF, IH(P), WB	β-APP full length (APP695, 751, 770); N-terminal truncated forms of β-APP; C-terminal membrane-anchored frag. of β-APP after α- or β-secretase cleavage. Not β-APP product N-terminal to the γ-secretase cleavage site	human, rat, mouse, pig (expect monkey, guinea pig and chicken)
β-APP (poly)	Histo-Line Cat# 36-6900	immunogen = synthetic peptide derived from C-terminus of human and mouse AβPP(APP695)	polyclonal rabbit (liquid, PBS, azide, affinity purified)	IH(P), IP, WB	full length APP; APLP; C99 C-terminal cleavage frag.	human, mouse
β-APP (poly)	Leinco Technologies cat# A162	immunogen = synthetic peptide corresp. to aa 653-662 of human APP or 1-10 of the 4K Aβ peptide generated by β- or γ-secretases	polyclonal rabbit (liquid, PBS, azide, affinity purified)	WB	β-APP	human, rat, mouse (other species not tested)
β-APP (LN27) IgG₁κ	Zymed/ Invitrogen Cat# 13-0200 References: Dreses-Werringloer, 2008 Li, 2003	Immunogen = recombinant-β-APP695; epitope = first 200 aa in N-terminus	monoclonal mouse (liquid, purified, PBS, azide)	ELISA, IB, IH(F, P), WB	all three forms of β-APP (APP695, 751, 770)	human
β-APP (poly)	Zymed/ Invitrogen Cat# 51-2700 (CT695) References: Muresan, 2009 Burton, 2008 Wahrle, 2008	Immunogen = synthetic peptide of 22 aa from the C-terminus of human β-APP	polyclonal rabbit (affinity purified, PBS, azide)	ELISA, IF, IH(F, P), WB	All three forms of full length APP and N-terminal truncated forms of β-APP. Detects C-terminal membrane fragment of β-APP that remains after α -or β-secretase cleavage	human, rat, mouse, pig
β-APP (poly)	Zymed/ Invitrogen Cat# 36-6900 Reference: Vieira SI, 2010	immunogen = synthetic peptide derived from the C-terminal region of the human and mouse amyloid-β precursor protein (APP695)	polyclonal rabbit (liquid, affinity purified, PBS, azide)	IH(P), IP, WB	APP full length (~100kDa) and C99 C-terminal cleavage frag (~10-12kDa); cross-react with APLP2	human, mouse
β-APP, KPI domain (mono) IgG₁	Chemicon and Upstate are Millipore cat# AB5354	immunogen = purified recombinant KPI domain of AβPP	monoclonal mouse (liquid, PBS, azide, purified)	ELISA, IH(F), WB	AβPP, KPI (Kunitz protease inhibitor) domain; native and recombinant	human (other species not tested)
AβPP (mono) IgG₁, κ	Abcam Cat# ab54813	immunogen = recombinant full length human amyloid beta precursor protein (AAH04369, 306 a.a) with a 26 kDa tag	monoclonal mouse (liquid, PBS, no preservative, protein A purified)	WB	recognizes AβPP	human
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (1.D5) IgG₁	Abcam Cat# ab77738	immunogen = synthetic peptide, corresp. to aa511 - 608 of human amyloid beta precursor protein A4 695	monoclonal rabbit (liquid, PBS, azide, protein A purified)	WB	reacts with the natural amyloid precursor protein A4 695	human, rat
AβPP (12F4) IgG₁	Abcam Cat# ab78314	immunogen = synthetic peptide: M F C G R L N corresp. to aa36-42 of human amyloid beta precursor protein, conj. to KLH	monoclonal mouse (liquid, ascites, azide)	IH(P), WB	recognizes AβPP	human, mouse
AβPP (2.F2.19B4) IgG₁	Abcam Cat# ab78271	immunogen = recombinant fragment corresp. to C-terminal aa643-695 of human amyloid beta precursor protein	monoclonal mouse (liquid, ascites, azide)	IH(P), WB	reacts with intact full length Amyloid β precursor protein and selectively with the cytoplasmic carboxyl fragment of amyloid β precursor protein 643-695	cynomolgus, monkey, human, chimpanzee, rat, mouse, pig, dog, cow, chicken (orangutan)
AβPP (Y188) IgG	Abcam Cat# AB32136	immunogen = synthetic peptide corresp. to residues in the NPXY motif of human Amyloid β A4 precusor (APP).	monoclonal rabbit (liquid, tris-glycine saline, glycerol, azide)	FC, IC, IH(P), IP, WB	recognizes Amyloid beta precursor protein and its isoforms. It will not recognize either of the two Amyloid-β protein variants, Amyloid-β 1-40 or Amyloid-β 1-42.	human, rat, mouse (others not tested)
AβPP (poly) IgG	Abcam Cat# AB12269	immunogen = synthetic peptide from within human KPI domain of human APP751/770, conj. to KLH	polyclonal rabbit (liquid, whole serum, azide)	IC, IH(P), IP, WB	recognises both APP751 and APP770 which contain the KPI domain	macaque, human, mouse (others not tested)
AβPP (poly) IgG	Abcam Cat# AB12270	immunogen = synthetic peptide from C-terminal human APP conj. to KLH	polyclonal rabbit (liquid, whole serum, azide)	IC, IP, WB	reacts with membrane-associated APP695, APP751 and APP770, as well as C-terminal membrane stubs	monkey, human, rat (others not tested)
AβPP (poly) IgG	Abcam Cat# AB12271	immunogen = synthetic peptide from C-terminal human APP conj. to KLH	polyclonal rabbit (liquid, whole serum, azide)	IC, IP, WB	reacts with APP695, APP751 and APP770	monkey, human, rat (not bovine; others not tested)
AβPP (poly) IgG	Abcam Cat# AB12268	immunogen = synthetic peptide from C-terminal corresp. to the OX-2 domain of human APP770, conj. to KLH	polyclonal rabbit (liquid, whole serum, azide)	IC, IP, WB	recognises the OX2 domain which is only present in APP770 in cells that express the recombinant APP770 (low endogenous levels)	macaque, human (others not tested)
AβPP (poly) IgG	Abcam Cat# ab17467	immunogen = synthetic peptide corresp. to residues surrounding T668 of human APP, conj. to KLH	polyclonal rabbit (liquid, Hepes, BSA, NaCl, glycerol, protein A purified)	ELISA, IH(P), WB	detects total levels of several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP695, APP770 and APP751	monkey, human, mouse (others not tested)
AβPP (poly) IgG	Abcam Cat# ab18813	immunogen = synthetic peptide (E V D A A V T P E E R H L S K) corresp. to aa 737-751 of human AβPP or aa 85 to 99 of the C99 fragment generated by β- secretase cleavage.	polyclonal rabbit (liquid, PBS, BSA, glycerol, thimerosal, affinity purified)	WB	recognizes AβPP and its isoforms; not the two Aβ protein variants, Aβ 1-40 or Aβ 1-42.	monkey, human, rat, mouse, chicken (others not tested)
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (poly) IgG	Abcam Cat# ab77758	immunogen = synthetic peptide: F C G R L N corresp. to aa37-42 of human amyloid beta precursor protein, conj. to KLH,	polyclonal rabbit (liquid, whole serum, azide)	ELISA, IH(P)	recognizes AβPP	human
AβPP (poly) IgG	Abcam Cat# ab77785	immunogen = synthetic peptide: P S L A L L L L A A W T V R, corresp. to aa 3-16 of mouse amyloid beta precursor protein	polyclonal rabbit (liquid, PBS, no preservative, protein A purified)	IH(F); not WB	reacts well with all isoforms of Amyloid beta precursor protein	rat, mouse (not human)
AβPP (mono) IgG_2a	Autogen Bioclear Cat# LS-C51594 manufacturer: LifeSpan Biosciences	immunogen = Ni-NTA purified truncated recombinant APP expressed in E. coli strain BL21 (DE3)	monoclonal mouse (liquid, PBS, azide, protein A purified)	ELISA, IH(F, P), WB	truncated APP recombinant protein (WB), human APP recombinant protein (ELISA), human Alzheimer brain tissue (IHC)	human, rat, mouse
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C44359 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa seq. 1-17 of human Amyloid Beta protein	monoclonal mouse (liquid, PBS, azide, protein G purified)	ELISA, IH(F, P), IP, WB	beta amyloid peptide, which is the major protein component of the amyloid cores in neuritic plaques	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20575 manufacturer: LifeSpan Biosciences	immunogen = human beta amyloid 1-40 (KLH coupled)	monoclonal mouse (liquid, ascites, glycerol, azide)	ELISA, IF, IH(F, P), WB	recognizes human β-amyloid1-40/42. Epitope: localized to the N-terminus of β-amyloid (1-13 aa of 1-40/42/43)	human, rat, mouse
AβPP (mono) IgG	Autogen Bioclear Cat# LS-C3644 manufacturer: LifeSpan Biosciences	immunogen = 21-aa peptide within the extracellular (18-38 aa), N-terminus of human APP695	monoclonal mouse (liquid, PBS, BSA, glycerol)	ELISA, WB	APP	human, rat, mouse
AβPP (mono) IgG₁, κ	Autogen Bioclear Cat# LS-C20593 manufacturer: LifeSpan Biosciences	immunogen = amyloid β-peptide (5-16) coupled to carrier	monoclonal mouse (liquid, PBS, azide, protein A/G purified)	ELISA	binding of this antibody to amyloid β-peptide depends on the presence of at least aa residues 10-16	human
AβPP (mono) IgG_2a, κ	Autogen Bioclear Cat# LS-C20592 manufacturer: LifeSpan Biosciences	immunogen = amyloid β-peptide (5-16) coupled to carrier	monoclonal mouse (liquid, PBS, azide, protein A/G purified)	ELISA	binding of this antibody to amyloid β-peptide depends on the presence of at least aa residues 5-9	human
AβPP (mono) IgG_2a, κ	Autogen Bioclear Cat# LS-C20591 manufacturer: LifeSpan Biosciences	immunogen = amyloid β-peptide (5-16) coupled to carrier	monoclonal mouse (liquid, PBS, azide, protein A/G purified)	ELISA	binding of this antibody to amyloid β-peptide depends on the presence of at least aa residues 10-16	human
AβPP (mono) IgG_2a, κ	Autogen Bioclear Cat# LS-C20590 manufacturer: LifeSpan Biosciences	immunogen = amyloid β-peptide (5-16) coupled to carrier	monoclonal mouse (liquid, PBS, azide, protein A/G purified)	ELISA	binding of this antibody to amyloid β-peptide depends on the presence of at least aa residues 9-10	human
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20581 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa1-42 of C-terminal human β-amyloid (KLH)	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, gel filtration)	ELISA, IF, WB	recognizes the C-terminus of human β-amyloid (aa1-42 isoform); no cross-react with β-amyloid (aa1-40 isoform)	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20576 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa 1-40 and aa 1-42 of C-terminal human β-amyloid; coupled with KLH	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, gel filtration)	ELISA, IF, WB	recognizes the C-terminus of human β-amyloid (aa1-40 and 1-42 isoform)	human
AβPP (mono) IgG₁, κ	Autogen Bioclear Cat# LS-C20574 manufacturer: LifeSpan Biosciences	immunogen = 28 aa synthetic peptide derived from the β-amyloid peptide	monoclonal mouse (liquid, PBS, azide, purified)	ELISA, IH(F, P), WB	reacts with the 4-5kD β-amyloid peptide (β-AP). High concentrations of antibody will also react with A-α, B-α and γ subunits of fibrinogen	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20566 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa 1-43 of C-terminal human β-amyloid	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, gel filtration)	ELISA, IF, WB	recognizes β-amyloid (aa1-43 isoform); no cross-react with β-amyloid (aa1-40 or 1-42 isoform)	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20552 manufacturer: LifeSpan Biosciences	immunogen = C-terminal of human β-amyloid	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, purified)	ELISA, IF, WB	recognizes the C-terminal of β-amyloid (aa1-40 isoform); no cross-react with β-amyloid (aa 1-42 isoform)	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20556 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to a portion of N-terminal of human β-amyloid (KLH)	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, gel filtration)	ELISA, IF, WB	recognizes the free N-terminus of human β-amyloid A4 as well as APP (amyloid precursor protein)	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20552 manufacturer: LifeSpan Biosciences	immunogen = C-terminal peptide of human beta-amyloid (KLH coupled)	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, gel filtration)	ELISA, IF, WB	recognizes the C-terminus of human β-amyloid (aa1-40); no cross reaction with β-amyloid aa 1-42	human
AβPP (mono) IgG₁, κ	Autogen Bioclear Cat# LS-C20559 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to residues 8-17 (S G Y E V H H Q K L) of the amyloid β-protein/amyloid A4 which is the same as aa660-669 of human amyloid A4/amyloid β- protein precursor	monoclonal mouse (liquid, supernatant, azide)	IH(F, P)	reacts with 4-5kD peptide derived from the amyloid- β-precursor protein	human
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20553 manufacturer: LifeSpan Biosciences	immunogen = C-terminal of human β-amyloid	monoclonal mouse (liquid, PBS, azide, sucrose, glycerol, purified, biotin)	ELISA, IF, WB	recognizes the C-terminal of β-amyloid (aa1-40 isoform); no cross-react with β-amyloid (aa 1-42 isoform)	human
AβPP (mono)	Autogen Bioclear Cat# LS-C20550 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to a portion of N-terminal of human -amyloid	monoclonal mouse (conj. biotin)	ELISA, WB	recognizes the free N-terminal of human βA4-amyloid polypeptide. Shows minor cross-reactivity with APP	human
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (mono)	Autogen Bioclear Cat# LS-C20578 manufacturer LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa 1-40 and aa 1-42 of C-terminal human -amyloid	monoclonal mouse (FITC conj.)	ELISA, IC, WB	recognizes human C-terminal β-amyloid (aa 1-40 and aa 1-42).	human
AβPP (mono) IgG₁, κ	Autogen Bioclear Cat# LS-C20585 manufacturer: Lifespan Biosciences	immunogen = 28aa synthetic peptide derived from the β-amyloid peptide.	monoclonal mouse (liquid, PBS, azide, purified)	ELISA, IH(F, P), WB	reacts with the 4-5kD β-amyloid peptide (β-AP); high concentrations of antibody will also react with A-α-, B-α- and γ subunits of fibrinogen.	human
AβPP (mono) IgG_2a, κ	Autogen Bioclear Cat# LS-C20538 manufacturer LifeSpan Biosciences	immunogen = amyloid precursor protein (APP) secreted by the EJ-1 bladder carcinoma cell line	monoclonal mouse (liquid, PBS, azide, glycerol, affinity purified)	ELISA, WB	recognizes secretory amyloid precursor protein (sAPP); no cross reaction with amyloid-beta protein 1-40	human
AβPP (mono) IgG	Autogen Bioclear Cat# LS-C20557 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa 36-42 of human amyloid β-x-42 peptide conj. to KLH	monoclonal mouse (liquid, ascites, azide)	IH(F, P), WB	recognizes amyloid β-x-42 polypeptide, Mr 4.5kD	human, mouse
AβPP (mono) IgG₁	Autogen Bioclear Cat# LS-C20482 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide Alz 90 (aa 511-608 of amyloid precursor protein pre A4695)	monoclonal mouse (liquid, PBS, azide, purified)	WB	natural amyloid precursor protein A4695	human, rat
AβPP (1B11F3) IgG_2a	Autogen Bioclear Cat# LS-C47167	immunogen = Ni-NTA purified truncated recombinant APP expressed in E. coli strain BL21 (DE3)	monoclonal mouse (liquid, affinity purified)	ELISA, WB	rhAβPP	human
AβPP (1B11F3) IgG_2a, κ	Autogen Bioclear Cat# LS-C82076 manufacturer: LifeSpan Biosciences	immunogen = Ni-NTA purified truncated recombinant APP expressed in E. coli strain BL21 (DE3)	monoclonal mouse (liquid, ascites, PBS, azide)	IH(F, P), WB	APP	human
AβPP (22C11) IgG₁	Autogen Bioclear Cat# LS-C84777 manufacturer: LifeSpan Biosciences	immunogen = purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein	monoclonal mouse			monkey, human, rat, mouse, porcine, dog, fish (various)
AβPP (2Q12) IgG₁	Autogen Bioclear Cat# LS-C20480 manufacturer: LifeSpan Biosciences	immunogen = purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein	monoclonal mouse (liquid, PBS, azide, purified)	IF, IH(P), WB	recognizes a N-terminal epitope on the pre-A4 molecule	monkey, human, rat, mouse, fish (various)
AβPP (33D3)	Autogen Bioclear Cat# LS-C53727 manufacturer LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa residues 1-16 of Aβ (β-amyloid) peptide	monoclonal mouse (liquid, PBS, azide, protein G purified)	ELISA, IH, IP, WB	recreacts with human Aβ peptide. The epitope lies within aa residues 3-8 of β-amyloid (Aβ) peptide	human
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (33D3) IgG_2b	Autogen Bioclear Cat# LS-C53958 manufacturer LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa residues 17-24 of Aβ (β-amyloid) peptide	monoclonal mouse (liquid, PBS, no preservative, protein A purified)	IH(F), WB	Aβ	human, rat, mouse
AβPP (40.1) IgM	Autogen Bioclear Cat# LS-C84781 manufacturer: LifeSpan Biosciences	immunogen = prokaryotic rh. fusion protein corresp. to the extracellular portion of APP between the Kunitz protease inhibitor domain and the β-amyloid region	monoclonal mouse
AβPP (6F/3D) IgG₁	Autogen Bioclear Cat# LS-C84807 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide representing a site on the β-amyloid molecule.	monoclonal mouse
AβPP (8A104)	Autogen Bioclear Cat# LS-C68977 manufacturer: LifeSpan Biosciences	immunogen = Ni-NTA purified truncated recombinant APP expressed in E. coli strain BL21 (DE3)	monoclonal mouse (liquid, PBS, NaCl, azide, glycerol, protein A purified)	ELISA, IH(F, P), WB	truncated APP recombinant protein (WB), human APP recombinant protein (ELISA), human Alzheimer brain tissue (IHC)	human
AβPP (8J259) IgG₁	Autogen Bioclear Cat# LS-C68959 manufacturer LifeSpan Biosciences	immunogen = synthetic peptide, corresp. to aa1-17 of human β- amyloid	monoclonal mouse (liquid, PBS, protein G purified)	IH(P), IP, WB	APP	human
AβPP (8J260) IgG	Autogen Bioclear Cat# LS-C68964 manufacturer: LifeSpan Biosciences		monoclonal rabbit (liquid, PBS, BSA, azide, affinity purified)	IH(F), IP, WB	APP	human, rat, mouse
AβPP (8J262) IgG₁	Autogen Bioclear Cat# LS-C68969 manufacturer: LifeSpan Biosciences	immunogen = human SAA and helix pomatia hemocyanin	monoclonal rabbit (liquid, PBS, BSA, azide, protein G purified)	IH(F, P)	reacts with all known SAA types	human
AβPP (8J263) IgG_2a	Autogen Bioclear Cat# LS-C68972 manufacturer: LifeSpan Biosciences	immunogen = human serum amyloid A (Apo-SAA)	monoclonal mouse (liquid, supernatant)	IH(F, P)	reacts with AA-type Amyloid depositions	human, rat
AβPP (8J264) IgG₁	Autogen Bioclear Cat# LS-C68986 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide: L E V P T D G N A G L L A E P Q I A M F C, corresp. to aa18-38 of human amyloid precursor protein	monoclonal mouse (liquid, PBS, no preservative, protein A purified)	IH(P), WB	APP	monkey, human, rat, guinea pig, mouse, pig, dog, chicken
AβPP (BAM01) IgG₁, κ	Autogen Bioclear Cat# LS-C87585	immunogen = synthetic peptide: S G Y E V H H Q K L, corresp. to aa660-669 of human APP (which is the same as aa8-17 of the mature amyloid protein)	monoclonal mouse (liquid, supernatant, azide)	IH(P)	AβPP	human
Antigen (Clone) IgG	Distributor (Producer)	Immunogen (Epitope)	Host (Formulation)	Methods glossary	Specificity	Reactivity top
AβPP (BAM01) IgG₁	Autogen Bioclear Cat# LS-C95588	immunogen = synthetic beta-amyloid peptide (1-40) conj. to KLH	monoclonal mouse (liquid, ascites, azide)	IH(P)	AβPP	human
AβPP (BAM01) IgG₁, κ	Autogen Bioclear Cat# LS-C87585	immunogen = synthetic peptide: S G Y E V H H Q K L, corresp. to aa660-669 of human APP (which is the same as aa8-17 of the mature amyloid protein)	monoclonal mouse (liquid, supernatant, azide)	IH(P)	AβPP	human
AβPP (EP1876Y) IgG	Autogen Bioclear Cat# LS-C50168 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to residues in human Beta-amyloid protein 40	monoclonal rabbit (liquid, BSA, supernatant, tris-glycine saline, glycerol, azide)	WB	recognizes Amyloid Beta protein (Abeta4) variant Abeta-40	human
AβPP (J4H9) IgG_2b, κ	Autogen Bioclear Cat# LS-B2970 manufacturer: LifeSpan Biosciences	immunogen = APP antibody was raised against recombinant human APP (18-289aa) purified from E. coli	monoclonal rabbit (liquid, PBS, azide)	ELISA, IH(P), WB	APP	human
AβPP (Y188) IgG	Autogen Bioclear Cat# LS-C49542 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to residues in the NPXY motif of human Amyloid Beta A4 precursor (APP)	monoclonal rabbit (liquid, Tris-glycine, NaCl, supernatant, azide, glycerol)	FC, IF, IH(P), IP, WB	recognizes APP and its isoforms; not either of the two amyloid beta protein (Abeta4) variants, Abeta 1-40 or Abeta 1-42	human, rat, mouse
AβPP (poly) IgG	Autogen Bioclear Cat# LS-C51557 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide R P G S G L T N I K conj. to KLH	polyclonal rabbit (liquid, PBS, azide, glycerol, affinity purified)	ELISA, IF, WB	recognizes recombinant APP and endogenous APP	human, rat, mouse
AβPP (poly) IgG	Autogen Bioclear Cat# LS-C51551 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa1-17 of beta-amyloid conj. to KLH	polyclonal rabbit (liquid, PBS, azide, affinity purified)	ELISA, IF, WB	beta-amyloid 1-40 and beta-amyloid 1-42 peptides	human
AβPP (poly) IgG	Autogen Bioclear Cat# LS-C51555 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide H M N V Q N G K W D S D P S G T K T C I conj. to KLH	polyclonal rabbit (liquid, PBS, glycerol, azide, affinity purified)	ELISA, IF, WB	recombinant APP, endogenous APP	human
AβPP (poly) IgG	Autogen Bioclear Cat# LS-C51552 manufacturer: LifeSpan Biosciences	immunogen = synthetic peptide corresp. to aa25-35 of human beta-Amyloid conjugated to KLH	polyclonal rabbit (liquid, PBS, glycerol, azide, affinity purified)	ELISA, WB	beta-amyloid 25-35 and beta-amyloid 1-40 and does not react with beta-amyloid 1-42	human




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